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Evidences for the Involvement of Monoaminergic and GABAergic Systems in Antidepressant-like Activity of Tinospora cordifolia in Mice.

Dhingra D, Goyal PK - Indian J Pharm Sci (2008)

Bottom Line: Petroleum ether extract at all three doses produced significant antidepressant-like effect in tail suspension test as well as in forced swim test and their efficacies were found to be comparable to imipramine (15 mg/kg, p.o.) and sertraline (20 mg/kg, p.o.).The antidepressant-like effect of the extract was significantly reversed by pretreatment of animals with prazosin (a α(1)-adrenoceptor antagonist), sulpiride (a selective dopamine D(2)-receptor antagonist), p-CPA (a serotonin synthesis inhibitor) and baclofen (GABA-B agonist), when tested in tail suspension test.Moreover, petroleum ether extract also reduced the mouse whole brain monoamine oxidase (MAO-A and MAO-B) activities as compared to control, resulting in increase in the levels of brain monoamines.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar-125001, India.

ABSTRACT
The present study was taken up to investigate the effect of petroleum ether extract of Tinospora cordifolia (Wild.) Miers, on depression in mice. The extract (50, 100 and 200 mg/kg, p.o.) was administered for 14 successive days to Swiss young albino mice (either sex) and evaluated for antidepressant-like activity using tail suspension test and forced swim test. Petroleum ether extract at all three doses produced significant antidepressant-like effect in tail suspension test as well as in forced swim test and their efficacies were found to be comparable to imipramine (15 mg/kg, p.o.) and sertraline (20 mg/kg, p.o.). The extract at a dose of 50 mg/kg showed most potent effect and did not show any significant change in locomotor functions of mice as compared to control. The antidepressant-like effect of the extract was significantly reversed by pretreatment of animals with prazosin (a α(1)-adrenoceptor antagonist), sulpiride (a selective dopamine D(2)-receptor antagonist), p-CPA (a serotonin synthesis inhibitor) and baclofen (GABA-B agonist), when tested in tail suspension test. Moreover, petroleum ether extract also reduced the mouse whole brain monoamine oxidase (MAO-A and MAO-B) activities as compared to control, resulting in increase in the levels of brain monoamines. Therefore, the extract may have potential therapeutic value for the management of depressive disorders.

No MeSH data available.


Related in: MedlinePlus

Effect of petroleum ether extract of T. cordifolia on MAO-A activityn=6 in each group; Values are in mean±SEM. Data was analyzed by one-way ANOVA followed by Dunnett's t-test. F (2,15)= 12.235; P<0.05, *P<0.05 when compared with vehicle treated group.
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Figure 0001: Effect of petroleum ether extract of T. cordifolia on MAO-A activityn=6 in each group; Values are in mean±SEM. Data was analyzed by one-way ANOVA followed by Dunnett's t-test. F (2,15)= 12.235; P<0.05, *P<0.05 when compared with vehicle treated group.

Mentions: Petroleum ether extract (50 mg/kg) administered for 14 consecutive days to mice, significantly reduced the brain MAO-A and MAO-B levels as compared to the respective vehicle treated groups. The efficacy of petroleum ether extract, was found to be comparable to imipramine (Figs. 1 and 2).


Evidences for the Involvement of Monoaminergic and GABAergic Systems in Antidepressant-like Activity of Tinospora cordifolia in Mice.

Dhingra D, Goyal PK - Indian J Pharm Sci (2008)

Effect of petroleum ether extract of T. cordifolia on MAO-A activityn=6 in each group; Values are in mean±SEM. Data was analyzed by one-way ANOVA followed by Dunnett's t-test. F (2,15)= 12.235; P<0.05, *P<0.05 when compared with vehicle treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040870&req=5

Figure 0001: Effect of petroleum ether extract of T. cordifolia on MAO-A activityn=6 in each group; Values are in mean±SEM. Data was analyzed by one-way ANOVA followed by Dunnett's t-test. F (2,15)= 12.235; P<0.05, *P<0.05 when compared with vehicle treated group.
Mentions: Petroleum ether extract (50 mg/kg) administered for 14 consecutive days to mice, significantly reduced the brain MAO-A and MAO-B levels as compared to the respective vehicle treated groups. The efficacy of petroleum ether extract, was found to be comparable to imipramine (Figs. 1 and 2).

Bottom Line: Petroleum ether extract at all three doses produced significant antidepressant-like effect in tail suspension test as well as in forced swim test and their efficacies were found to be comparable to imipramine (15 mg/kg, p.o.) and sertraline (20 mg/kg, p.o.).The antidepressant-like effect of the extract was significantly reversed by pretreatment of animals with prazosin (a α(1)-adrenoceptor antagonist), sulpiride (a selective dopamine D(2)-receptor antagonist), p-CPA (a serotonin synthesis inhibitor) and baclofen (GABA-B agonist), when tested in tail suspension test.Moreover, petroleum ether extract also reduced the mouse whole brain monoamine oxidase (MAO-A and MAO-B) activities as compared to control, resulting in increase in the levels of brain monoamines.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar-125001, India.

ABSTRACT
The present study was taken up to investigate the effect of petroleum ether extract of Tinospora cordifolia (Wild.) Miers, on depression in mice. The extract (50, 100 and 200 mg/kg, p.o.) was administered for 14 successive days to Swiss young albino mice (either sex) and evaluated for antidepressant-like activity using tail suspension test and forced swim test. Petroleum ether extract at all three doses produced significant antidepressant-like effect in tail suspension test as well as in forced swim test and their efficacies were found to be comparable to imipramine (15 mg/kg, p.o.) and sertraline (20 mg/kg, p.o.). The extract at a dose of 50 mg/kg showed most potent effect and did not show any significant change in locomotor functions of mice as compared to control. The antidepressant-like effect of the extract was significantly reversed by pretreatment of animals with prazosin (a α(1)-adrenoceptor antagonist), sulpiride (a selective dopamine D(2)-receptor antagonist), p-CPA (a serotonin synthesis inhibitor) and baclofen (GABA-B agonist), when tested in tail suspension test. Moreover, petroleum ether extract also reduced the mouse whole brain monoamine oxidase (MAO-A and MAO-B) activities as compared to control, resulting in increase in the levels of brain monoamines. Therefore, the extract may have potential therapeutic value for the management of depressive disorders.

No MeSH data available.


Related in: MedlinePlus