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Pharmacological evaluation of Bioactive Principle of Turnera aphrodisiaca.

Kumar S, Madaan R, Sharma A - Indian J Pharm Sci (2008)

Bottom Line: Apigenin significantly increased head dipping in hole board test.At a higher dose (about 12 fold the anxiolytic dose), apigenin showed mild sedative activity in actophotometer as it decreased activity scores.It (2, 5 or 10 mg/kg) was found to be devoid of anticonvulsant, antidepressant and antistress activity in MES-induced convulsion test, despair swim test and cold swimming endurance test, respectively.

View Article: PubMed Central - PubMed

Affiliation: S. D. College of Pharmacy, Barnala-148 101, Punjab, India.

ABSTRACT
In the present investigation, pharmacological evaluation of apigenin, a bioactive principle of Turnera aphrodisiaca Ward (Turneraceae) was carried out. Apigenin was evaluated for antianxiety activity at a dose of 2 mg/kg using well established models of anxiety, the hole board test, light/dark test and mirrored chamber test. Apigenin significantly increased head dipping in hole board test. Further, apigenin increased latency to leave light zone and the time spent in light compartment of light/dark model of anxiety. Apigenin also decreased the latency time to enter the mirrored chamber, and increased the total time spent/number of entries in the mirrored chamber with respect to control. All these observations confirmed the anxiolytic activity of apigenin. At a higher dose (about 12 fold the anxiolytic dose), apigenin showed mild sedative activity in actophotometer as it decreased activity scores. It (2, 5 or 10 mg/kg) was found to be devoid of anticonvulsant, antidepressant and antistress activity in MES-induced convulsion test, despair swim test and cold swimming endurance test, respectively. In tail immersion test for six hours, apigenin exhibited excellent dose dependent analgesic activity, which was comparable to that of morphine sulphate (5 mg/kg). Maximum activity was observed 30 min after the administration of 10 mg/kg dose of apigenin.

No MeSH data available.


Related in: MedlinePlus

Antianxiety activity of apigenin using hole board test.The data is expressed as Mean±SEM of n=5 observations; *P<0.05 vs. control; ANOVA followed by Studentized Tukey's test. Vehicle; 2 mg/kg; 2 mg/kg
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Figure 0001: Antianxiety activity of apigenin using hole board test.The data is expressed as Mean±SEM of n=5 observations; *P<0.05 vs. control; ANOVA followed by Studentized Tukey's test. Vehicle; 2 mg/kg; 2 mg/kg

Mentions: Our previous work based on bioactivity-directed fractionation of bioactive methanol extract of T. aphrodisiaca aerial parts afforded an anxiolytic flavone, apigenin which exhibited significant anti-anxiety activity at a dose of 2 mg/kg using elevated plus maze model of anxiety18. To substantiate data, apigenin was further evaluated for antianxiety activity in other well established models of anxiety, and also for various CNS-related bioactivities. Apigenin was evaluated for anti-anxiety activity at a dose of 2 mg/kg using well established models of anxiety, i.e., hole board test, light/dark test and mirrored chamber test. Results of hole board, light/dark and mirrored chamber tests have been shown in figs. 1–3, respectively. Apigenin significantly increased head dipping in hole board test. Further, apigenin increased latency to leave light zone and the time spent in light compartment of light/dark model of anxiety. Apigenin also decreased the latency time to enter the mirrored chamber, and increased the total time spent/number of entries in the mirrored chamber with respect to control. All these observations confirmed the anxiolytic activity of apigenin.


Pharmacological evaluation of Bioactive Principle of Turnera aphrodisiaca.

Kumar S, Madaan R, Sharma A - Indian J Pharm Sci (2008)

Antianxiety activity of apigenin using hole board test.The data is expressed as Mean±SEM of n=5 observations; *P<0.05 vs. control; ANOVA followed by Studentized Tukey's test. Vehicle; 2 mg/kg; 2 mg/kg
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040867&req=5

Figure 0001: Antianxiety activity of apigenin using hole board test.The data is expressed as Mean±SEM of n=5 observations; *P<0.05 vs. control; ANOVA followed by Studentized Tukey's test. Vehicle; 2 mg/kg; 2 mg/kg
Mentions: Our previous work based on bioactivity-directed fractionation of bioactive methanol extract of T. aphrodisiaca aerial parts afforded an anxiolytic flavone, apigenin which exhibited significant anti-anxiety activity at a dose of 2 mg/kg using elevated plus maze model of anxiety18. To substantiate data, apigenin was further evaluated for antianxiety activity in other well established models of anxiety, and also for various CNS-related bioactivities. Apigenin was evaluated for anti-anxiety activity at a dose of 2 mg/kg using well established models of anxiety, i.e., hole board test, light/dark test and mirrored chamber test. Results of hole board, light/dark and mirrored chamber tests have been shown in figs. 1–3, respectively. Apigenin significantly increased head dipping in hole board test. Further, apigenin increased latency to leave light zone and the time spent in light compartment of light/dark model of anxiety. Apigenin also decreased the latency time to enter the mirrored chamber, and increased the total time spent/number of entries in the mirrored chamber with respect to control. All these observations confirmed the anxiolytic activity of apigenin.

Bottom Line: Apigenin significantly increased head dipping in hole board test.At a higher dose (about 12 fold the anxiolytic dose), apigenin showed mild sedative activity in actophotometer as it decreased activity scores.It (2, 5 or 10 mg/kg) was found to be devoid of anticonvulsant, antidepressant and antistress activity in MES-induced convulsion test, despair swim test and cold swimming endurance test, respectively.

View Article: PubMed Central - PubMed

Affiliation: S. D. College of Pharmacy, Barnala-148 101, Punjab, India.

ABSTRACT
In the present investigation, pharmacological evaluation of apigenin, a bioactive principle of Turnera aphrodisiaca Ward (Turneraceae) was carried out. Apigenin was evaluated for antianxiety activity at a dose of 2 mg/kg using well established models of anxiety, the hole board test, light/dark test and mirrored chamber test. Apigenin significantly increased head dipping in hole board test. Further, apigenin increased latency to leave light zone and the time spent in light compartment of light/dark model of anxiety. Apigenin also decreased the latency time to enter the mirrored chamber, and increased the total time spent/number of entries in the mirrored chamber with respect to control. All these observations confirmed the anxiolytic activity of apigenin. At a higher dose (about 12 fold the anxiolytic dose), apigenin showed mild sedative activity in actophotometer as it decreased activity scores. It (2, 5 or 10 mg/kg) was found to be devoid of anticonvulsant, antidepressant and antistress activity in MES-induced convulsion test, despair swim test and cold swimming endurance test, respectively. In tail immersion test for six hours, apigenin exhibited excellent dose dependent analgesic activity, which was comparable to that of morphine sulphate (5 mg/kg). Maximum activity was observed 30 min after the administration of 10 mg/kg dose of apigenin.

No MeSH data available.


Related in: MedlinePlus