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Evaluation of hydrophobic nanoparticulate delivery system for insulin.

Singnurkar PS, Gidwani SK - Indian J Pharm Sci (2008)

Bottom Line: Insulin encapsulation efficiency was 95.7±1.2%.Insulin hydrophobic nanoparticles suppressed insulin release promoted sustained release in pH 7.4 phosphate buffer and shown to protect insulin from enzymatic degradation in vitro in presence of chymotripsin.Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: USV Limited, B. S. D. Marg, Govandi, Mumbai-400 088, India.

ABSTRACT
Insulin loaded hydrophobic nanoparticles were prepared by solvent diffusion followed by lyophilization. Nanoparticles were characterized for mean size by dynamic laser scattering and for shape by scanning electron microscopy. Insulin encapsulation efficiency, in vitro stability of nanoparticles in presence of proteolytic enzymes and in vitro release were determined by high pressure liquid chromatography analysis. The biological activity insulin from the nanopraticles was estimated by enzyme-linked immunosorbant assay and in vivo using Wister diabetic rats. Nanoparticles ranged 0.526±0.071 μm in diameter. Insulin encapsulation efficiency was 95.7±1.2%. Insulin hydrophobic nanoparticles suppressed insulin release promoted sustained release in pH 7.4 phosphate buffer and shown to protect insulin from enzymatic degradation in vitro in presence of chymotripsin. Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro.

No MeSH data available.


Scanning electron microscopy of insulin nanoparticles. Scanning electron micrograph of human insulin nanoparticles at 10,000X, white spherical particles are observed.
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Figure 0001: Scanning electron microscopy of insulin nanoparticles. Scanning electron micrograph of human insulin nanoparticles at 10,000X, white spherical particles are observed.

Mentions: After lyophilization, a white, extremely porous, fragile and light weight cake was observed. The extremely porous and fluppy cake formation after lyophilization can be attributed to the use of t-butanol as a solvent. t-Butanol is a versatile lyophilization medium as it has very high vapor pressure (26.8 mm Hg at 20°) and high freezing point (24°)13. The mean diameter of the human insulin nanoparticles did not display large variation, the mean particle size was about 0.526±0.071 μm. The SEM images confirm that the nanoparticles are circular in shape and well dispersed and separated on the surface (fig. 1).


Evaluation of hydrophobic nanoparticulate delivery system for insulin.

Singnurkar PS, Gidwani SK - Indian J Pharm Sci (2008)

Scanning electron microscopy of insulin nanoparticles. Scanning electron micrograph of human insulin nanoparticles at 10,000X, white spherical particles are observed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040864&req=5

Figure 0001: Scanning electron microscopy of insulin nanoparticles. Scanning electron micrograph of human insulin nanoparticles at 10,000X, white spherical particles are observed.
Mentions: After lyophilization, a white, extremely porous, fragile and light weight cake was observed. The extremely porous and fluppy cake formation after lyophilization can be attributed to the use of t-butanol as a solvent. t-Butanol is a versatile lyophilization medium as it has very high vapor pressure (26.8 mm Hg at 20°) and high freezing point (24°)13. The mean diameter of the human insulin nanoparticles did not display large variation, the mean particle size was about 0.526±0.071 μm. The SEM images confirm that the nanoparticles are circular in shape and well dispersed and separated on the surface (fig. 1).

Bottom Line: Insulin encapsulation efficiency was 95.7±1.2%.Insulin hydrophobic nanoparticles suppressed insulin release promoted sustained release in pH 7.4 phosphate buffer and shown to protect insulin from enzymatic degradation in vitro in presence of chymotripsin.Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: USV Limited, B. S. D. Marg, Govandi, Mumbai-400 088, India.

ABSTRACT
Insulin loaded hydrophobic nanoparticles were prepared by solvent diffusion followed by lyophilization. Nanoparticles were characterized for mean size by dynamic laser scattering and for shape by scanning electron microscopy. Insulin encapsulation efficiency, in vitro stability of nanoparticles in presence of proteolytic enzymes and in vitro release were determined by high pressure liquid chromatography analysis. The biological activity insulin from the nanopraticles was estimated by enzyme-linked immunosorbant assay and in vivo using Wister diabetic rats. Nanoparticles ranged 0.526±0.071 μm in diameter. Insulin encapsulation efficiency was 95.7±1.2%. Insulin hydrophobic nanoparticles suppressed insulin release promoted sustained release in pH 7.4 phosphate buffer and shown to protect insulin from enzymatic degradation in vitro in presence of chymotripsin. Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro.

No MeSH data available.