Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck (SCCHN) cell lines.
Bottom Line: Of the receptors, transcript expression of CCR1, CCR2, CCR3, CCR5, CCR7, CCXR2, and CCXR3 was not detected, and CCR6, CXCR1, and CXCR4 expression was restricted to few tumor cells.Radiation caused up- and down-regulation with respect to chemokine expressions, while for chemokine receptor expressions down-regulations were prevailing.CXCL1 and CXCL12 protein expression corresponded well with the mRNA expression.
Affiliation: Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany.Show MeSH
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Mentions: To test whether mRNA transcript expression was translated into protein, the presence of CXCL1 and CXCL12 was determined in cell culture supernatants by ELISA. Figure 5a, b show that the protein expression matches the mRNA transcript data well; however, no significant differences between irradiated and sham-irradiated cells were detected. Furthermore, Fig. 6 demonstrates that CXCL12 accumulates in irradiated cell culture supernatants over time, the increase being significant for tumor (BW-225) and normal (DF-19) cells.Fig. 5
Affiliation: Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany.