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Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck (SCCHN) cell lines.

Wolff HA, Rolke D, Rave-Fränk M, Schirmer M, Eicheler W, Doerfler A, Hille A, Hess CF, Matthias C, Rödel RM, Christiansen H - Radiat Environ Biophys (2010)

Bottom Line: Of the receptors, transcript expression of CCR1, CCR2, CCR3, CCR5, CCR7, CCXR2, and CCXR3 was not detected, and CCR6, CXCR1, and CXCR4 expression was restricted to few tumor cells.Radiation caused up- and down-regulation with respect to chemokine expressions, while for chemokine receptor expressions down-regulations were prevailing.CXCL1 and CXCL12 protein expression corresponded well with the mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany.

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CXCL12 protein expression in cell culture supernatants of BW-225 tumor and DF-19 normal cell lines 6, 24, and 48 h after irradiation. Data are normalized for CXCL12 content before irradiation; error bars represent standard errors of 3 measurements. The CXCL12 accumulation is statistically significant with P = <0.001
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Fig6: CXCL12 protein expression in cell culture supernatants of BW-225 tumor and DF-19 normal cell lines 6, 24, and 48 h after irradiation. Data are normalized for CXCL12 content before irradiation; error bars represent standard errors of 3 measurements. The CXCL12 accumulation is statistically significant with P = <0.001

Mentions: To test whether mRNA transcript expression was translated into protein, the presence of CXCL1 and CXCL12 was determined in cell culture supernatants by ELISA. Figure 5a, b show that the protein expression matches the mRNA transcript data well; however, no significant differences between irradiated and sham-irradiated cells were detected. Furthermore, Fig. 6 demonstrates that CXCL12 accumulates in irradiated cell culture supernatants over time, the increase being significant for tumor (BW-225) and normal (DF-19) cells.Fig. 5


Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck (SCCHN) cell lines.

Wolff HA, Rolke D, Rave-Fränk M, Schirmer M, Eicheler W, Doerfler A, Hille A, Hess CF, Matthias C, Rödel RM, Christiansen H - Radiat Environ Biophys (2010)

CXCL12 protein expression in cell culture supernatants of BW-225 tumor and DF-19 normal cell lines 6, 24, and 48 h after irradiation. Data are normalized for CXCL12 content before irradiation; error bars represent standard errors of 3 measurements. The CXCL12 accumulation is statistically significant with P = <0.001
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3040826&req=5

Fig6: CXCL12 protein expression in cell culture supernatants of BW-225 tumor and DF-19 normal cell lines 6, 24, and 48 h after irradiation. Data are normalized for CXCL12 content before irradiation; error bars represent standard errors of 3 measurements. The CXCL12 accumulation is statistically significant with P = <0.001
Mentions: To test whether mRNA transcript expression was translated into protein, the presence of CXCL1 and CXCL12 was determined in cell culture supernatants by ELISA. Figure 5a, b show that the protein expression matches the mRNA transcript data well; however, no significant differences between irradiated and sham-irradiated cells were detected. Furthermore, Fig. 6 demonstrates that CXCL12 accumulates in irradiated cell culture supernatants over time, the increase being significant for tumor (BW-225) and normal (DF-19) cells.Fig. 5

Bottom Line: Of the receptors, transcript expression of CCR1, CCR2, CCR3, CCR5, CCR7, CCXR2, and CCXR3 was not detected, and CCR6, CXCR1, and CXCR4 expression was restricted to few tumor cells.Radiation caused up- and down-regulation with respect to chemokine expressions, while for chemokine receptor expressions down-regulations were prevailing.CXCL1 and CXCL12 protein expression corresponded well with the mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany.

Show MeSH
Related in: MedlinePlus