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Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck (SCCHN) cell lines.

Wolff HA, Rolke D, Rave-Fränk M, Schirmer M, Eicheler W, Doerfler A, Hille A, Hess CF, Matthias C, Rödel RM, Christiansen H - Radiat Environ Biophys (2010)

Bottom Line: Of the receptors, transcript expression of CCR1, CCR2, CCR3, CCR5, CCR7, CCXR2, and CCXR3 was not detected, and CCR6, CXCR1, and CXCR4 expression was restricted to few tumor cells.Radiation caused up- and down-regulation with respect to chemokine expressions, while for chemokine receptor expressions down-regulations were prevailing.CXCL1 and CXCL12 protein expression corresponded well with the mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany.

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CXC-chemokine transcript expression relative to housekeeping genes in tumor and normal cell lines; a CXCL10, b CXCL11, c CXCL9, d CXCL12. Greater than twofold expression changes were regarded as significant and are marked by asterisks
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Fig3: CXC-chemokine transcript expression relative to housekeeping genes in tumor and normal cell lines; a CXCL10, b CXCL11, c CXCL9, d CXCL12. Greater than twofold expression changes were regarded as significant and are marked by asterisks

Mentions: Practically, all cell lines expressed the CC-chemokines CCL5 and CCL20, while CCL2 was expressed in normal cells and in some of the tumor cell lines (BHY, CAL27, CAL33, HSC4, SAS, BW-225, ZMK-1) (Fig. 1a–c). Likewise, the CXC-chemokines CXCL1, CXCL2, CXCL3, CXCL10, and CXCL11 were expressed in the vast majority of the cell lines, while the expression of CXCL9 and CXCL12 was restricted to fibroblasts and the tumor cell lines CAL27, CAL33, XF354, BW-225, and GR-145-I/II, HK-147, BW-225, respectively. Data representing transcript expressions of CXCL1, 2, 3 are given in Fig. 2 a–c; data representing transcript expressions of CXCL10, 11, 9, 12 are given in Fig. 3 a–d. None of the analyzed cell lines expressed the chemokines CCL3, CCL4, and CCL19.Fig. 2


Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck (SCCHN) cell lines.

Wolff HA, Rolke D, Rave-Fränk M, Schirmer M, Eicheler W, Doerfler A, Hille A, Hess CF, Matthias C, Rödel RM, Christiansen H - Radiat Environ Biophys (2010)

CXC-chemokine transcript expression relative to housekeeping genes in tumor and normal cell lines; a CXCL10, b CXCL11, c CXCL9, d CXCL12. Greater than twofold expression changes were regarded as significant and are marked by asterisks
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040826&req=5

Fig3: CXC-chemokine transcript expression relative to housekeeping genes in tumor and normal cell lines; a CXCL10, b CXCL11, c CXCL9, d CXCL12. Greater than twofold expression changes were regarded as significant and are marked by asterisks
Mentions: Practically, all cell lines expressed the CC-chemokines CCL5 and CCL20, while CCL2 was expressed in normal cells and in some of the tumor cell lines (BHY, CAL27, CAL33, HSC4, SAS, BW-225, ZMK-1) (Fig. 1a–c). Likewise, the CXC-chemokines CXCL1, CXCL2, CXCL3, CXCL10, and CXCL11 were expressed in the vast majority of the cell lines, while the expression of CXCL9 and CXCL12 was restricted to fibroblasts and the tumor cell lines CAL27, CAL33, XF354, BW-225, and GR-145-I/II, HK-147, BW-225, respectively. Data representing transcript expressions of CXCL1, 2, 3 are given in Fig. 2 a–c; data representing transcript expressions of CXCL10, 11, 9, 12 are given in Fig. 3 a–d. None of the analyzed cell lines expressed the chemokines CCL3, CCL4, and CCL19.Fig. 2

Bottom Line: Of the receptors, transcript expression of CCR1, CCR2, CCR3, CCR5, CCR7, CCXR2, and CCXR3 was not detected, and CCR6, CXCR1, and CXCR4 expression was restricted to few tumor cells.Radiation caused up- and down-regulation with respect to chemokine expressions, while for chemokine receptor expressions down-regulations were prevailing.CXCL1 and CXCL12 protein expression corresponded well with the mRNA expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy and Radiation Oncology, Universitätsmedizin Göttingen, Göttingen, Germany.

Show MeSH
Related in: MedlinePlus