Limits...
Ferritin immobilization on patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces from colloid system.

Chen TY, Chen JK - Colloid Polym Sci (2011)

Bottom Line: The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system.The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system.Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei, 106 Taiwan Republic of China.

ABSTRACT
In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

No MeSH data available.


Related in: MedlinePlus

a, b SEM images and c, d XPS N 1s core-level spectra of a PHEMA surface prepared through graft polymerization for 24 h and a, c immersion into a mixture of water and MeOH containing dispersed ferritin for 1 h and b, d then immersion into n-hexane for 1 h
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3040805&req=5

Fig8: a, b SEM images and c, d XPS N 1s core-level spectra of a PHEMA surface prepared through graft polymerization for 24 h and a, c immersion into a mixture of water and MeOH containing dispersed ferritin for 1 h and b, d then immersion into n-hexane for 1 h

Mentions: We exploited the OH groups of the PHEMA brushes obtained after immersion in water as “tentacles” to capture ferritin from aqueous solution [9, 34]. In this study, the “tentacles” behavior of PHEMA brush was applied in various fluidic systems for biochip application. Figure 8a displays an SEM image of a PHEMA surface prepared through graft polymerization for 24 h and then injected aqueous solution containing dispersed ferritin through the surface by micropump. Ferritin complexes having radii of 13 nm are clearly visible on the PHEMA surface, which adsorbed on the surface for all regions because of the protein shell of ferritins. The surface presenting the patterned PHEMA brushes was under aqueous solution containing dispersed ferritin, such that the OH groups of the surface were raised into the solution. It is generally difficult to control protein adsorption on solid surfaces because nonspecific protein adsorption is often the first phenomenon that occurs when the surface comes into contact with a physiological environment. The PHEMA brush captured the ferritin units through entanglement of the OH groups of the PHEMA brushes with the protein sheaths of the ferritin complexes. Next, we injected n-hexane through the sample to transform the surface from a brush-like to a mushroom-like structure, causing the OH groups of the PHEMA brushes to become buried—along with the associated ferritin units—within the PHEMA thin film to form a hydrophilic domain in which the ferritin units were trapped.Fig. 8


Ferritin immobilization on patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces from colloid system.

Chen TY, Chen JK - Colloid Polym Sci (2011)

a, b SEM images and c, d XPS N 1s core-level spectra of a PHEMA surface prepared through graft polymerization for 24 h and a, c immersion into a mixture of water and MeOH containing dispersed ferritin for 1 h and b, d then immersion into n-hexane for 1 h
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040805&req=5

Fig8: a, b SEM images and c, d XPS N 1s core-level spectra of a PHEMA surface prepared through graft polymerization for 24 h and a, c immersion into a mixture of water and MeOH containing dispersed ferritin for 1 h and b, d then immersion into n-hexane for 1 h
Mentions: We exploited the OH groups of the PHEMA brushes obtained after immersion in water as “tentacles” to capture ferritin from aqueous solution [9, 34]. In this study, the “tentacles” behavior of PHEMA brush was applied in various fluidic systems for biochip application. Figure 8a displays an SEM image of a PHEMA surface prepared through graft polymerization for 24 h and then injected aqueous solution containing dispersed ferritin through the surface by micropump. Ferritin complexes having radii of 13 nm are clearly visible on the PHEMA surface, which adsorbed on the surface for all regions because of the protein shell of ferritins. The surface presenting the patterned PHEMA brushes was under aqueous solution containing dispersed ferritin, such that the OH groups of the surface were raised into the solution. It is generally difficult to control protein adsorption on solid surfaces because nonspecific protein adsorption is often the first phenomenon that occurs when the surface comes into contact with a physiological environment. The PHEMA brush captured the ferritin units through entanglement of the OH groups of the PHEMA brushes with the protein sheaths of the ferritin complexes. Next, we injected n-hexane through the sample to transform the surface from a brush-like to a mushroom-like structure, causing the OH groups of the PHEMA brushes to become buried—along with the associated ferritin units—within the PHEMA thin film to form a hydrophilic domain in which the ferritin units were trapped.Fig. 8

Bottom Line: The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system.The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system.Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei, 106 Taiwan Republic of China.

ABSTRACT
In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

No MeSH data available.


Related in: MedlinePlus