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Ferritin immobilization on patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces from colloid system.

Chen TY, Chen JK - Colloid Polym Sci (2011)

Bottom Line: The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system.The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system.Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei, 106 Taiwan Republic of China.

ABSTRACT
In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

No MeSH data available.


Related in: MedlinePlus

XPS O 1s core-level spectra of the surfaces of the a pristine Si(100) and b Si-OPT
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Fig3: XPS O 1s core-level spectra of the surfaces of the a pristine Si(100) and b Si-OPT

Mentions: To prepare polymer brushes on the Si surface, it was necessary for us to immobilize a uniform and dense layer of initiators on the Si surface. The chemical compositions of the pristine Si(100) surface and the Si surfaces at various stages during the surface modification process were determined using XPS. Two peak components at binding energies (BE) of approximately 99 and 103 eV (attributable to Si–Si and Si–O species, respectively) appear in the Si 2p core-level spectrum of the pristine Si(100) surface (Fig. 2a). Treatment of the pristine Si(100) surface with HMDS passivated the native oxide layer and yielded a Si–C surface. The disappearance of the signal for the Si–O species at a BE of 103 eV confirmed that the Si surface was ideally carbon-terminated after HMDS treatment [35]. OPT of the Si surface removed the Si–C layer to activate the Si surface with Si–O species, which appeared as a signal at a BE of 103 eV in Fig. 2b. The weakened signal for the Si–O species at a BE of 103 eV confirmed that the Si surface was ideally carbon-terminated on the initiator (BPOTS)-functionalized Si surface (Fig. 2c). The disappearance of the signal for the Si–O species (BE = 103 eV) and the weakened signal for the Si–Si species (BE = 99 eV) on the surface presenting PHEMA brushes confirmed that the Si surface was ideally covered with the grafted PHEMA layer (Fig. 2d). Figure 3 displays the O 1s core-level spectra of the Si–OH surface after subjecting the pristine Si(100) surface to OPT for 20 s. The O element component increases from 31% to 65%, indicating the increased abundance of OH groups on the Si-OPT surface after OPT. Figure 4a displays the C 1s core-level spectra of the Si–OH surface after being subjected to BPOTS treatment. We curve-fitted the C 1s core-level spectra of the initiator (BPOTS)-functionalized Si surfaces to three peak components having BEs of approximately 284.2, 285.7, and 288.8 eV, attributable to C–H, C–O, and O = C–O species, respectively. Additionally, the presence in the Br 2p core-level spectrum of a signal at a BE of approximately 72.6 eV for the BPOTS-functionalized Si surface (Fig. 4b) indicated that the BPOTS species had been immobilized successfully on the Si surface.Fig. 2


Ferritin immobilization on patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces from colloid system.

Chen TY, Chen JK - Colloid Polym Sci (2011)

XPS O 1s core-level spectra of the surfaces of the a pristine Si(100) and b Si-OPT
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040805&req=5

Fig3: XPS O 1s core-level spectra of the surfaces of the a pristine Si(100) and b Si-OPT
Mentions: To prepare polymer brushes on the Si surface, it was necessary for us to immobilize a uniform and dense layer of initiators on the Si surface. The chemical compositions of the pristine Si(100) surface and the Si surfaces at various stages during the surface modification process were determined using XPS. Two peak components at binding energies (BE) of approximately 99 and 103 eV (attributable to Si–Si and Si–O species, respectively) appear in the Si 2p core-level spectrum of the pristine Si(100) surface (Fig. 2a). Treatment of the pristine Si(100) surface with HMDS passivated the native oxide layer and yielded a Si–C surface. The disappearance of the signal for the Si–O species at a BE of 103 eV confirmed that the Si surface was ideally carbon-terminated after HMDS treatment [35]. OPT of the Si surface removed the Si–C layer to activate the Si surface with Si–O species, which appeared as a signal at a BE of 103 eV in Fig. 2b. The weakened signal for the Si–O species at a BE of 103 eV confirmed that the Si surface was ideally carbon-terminated on the initiator (BPOTS)-functionalized Si surface (Fig. 2c). The disappearance of the signal for the Si–O species (BE = 103 eV) and the weakened signal for the Si–Si species (BE = 99 eV) on the surface presenting PHEMA brushes confirmed that the Si surface was ideally covered with the grafted PHEMA layer (Fig. 2d). Figure 3 displays the O 1s core-level spectra of the Si–OH surface after subjecting the pristine Si(100) surface to OPT for 20 s. The O element component increases from 31% to 65%, indicating the increased abundance of OH groups on the Si-OPT surface after OPT. Figure 4a displays the C 1s core-level spectra of the Si–OH surface after being subjected to BPOTS treatment. We curve-fitted the C 1s core-level spectra of the initiator (BPOTS)-functionalized Si surfaces to three peak components having BEs of approximately 284.2, 285.7, and 288.8 eV, attributable to C–H, C–O, and O = C–O species, respectively. Additionally, the presence in the Br 2p core-level spectrum of a signal at a BE of approximately 72.6 eV for the BPOTS-functionalized Si surface (Fig. 4b) indicated that the BPOTS species had been immobilized successfully on the Si surface.Fig. 2

Bottom Line: The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system.The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system.Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei, 106 Taiwan Republic of China.

ABSTRACT
In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

No MeSH data available.


Related in: MedlinePlus