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Ferritin immobilization on patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces from colloid system.

Chen TY, Chen JK - Colloid Polym Sci (2011)

Bottom Line: The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system.The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system.Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei, 106 Taiwan Republic of China.

ABSTRACT
In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

No MeSH data available.


Related in: MedlinePlus

Schematic representation of the strategy for ferritin capture. (I) The sample surface presenting the patterned PHEMA brushes is immersed for 1 h into a mixture of water and MeOH containing dispersed ferritin. (II) The sample surface is immersed into n-hexane to transform it from a brush-like to a mushroom-like structure, with the OH groups of the PHEMA brushes becoming buried within the PHEMA thin film to form hydrophilic domains. (III) The ferritin species on the PHEMA thin-film surface are removed through degradation of the protein sheath under O2 in an oven at approximately 500 °C to observe the ferrihydrite cores
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Fig1: Schematic representation of the strategy for ferritin capture. (I) The sample surface presenting the patterned PHEMA brushes is immersed for 1 h into a mixture of water and MeOH containing dispersed ferritin. (II) The sample surface is immersed into n-hexane to transform it from a brush-like to a mushroom-like structure, with the OH groups of the PHEMA brushes becoming buried within the PHEMA thin film to form hydrophilic domains. (III) The ferritin species on the PHEMA thin-film surface are removed through degradation of the protein sheath under O2 in an oven at approximately 500 °C to observe the ferrihydrite cores

Mentions: Ferritin (diameter of 13 nm; Fe core diameter of 7 nm; protein shell of 6 nm) was obtained from MP Biomedicals. Figure 1 displays the strategy used for ferritin capture. In Fig. 1 (I), the ferritin solution (good solvent for PHEMA brush) was injected into the channel surfaces patterned with PHEMA brushes by micropump. Ferritins adsorbed on the surface for all regions because of the protein shell of ferritins. In Fig. 1 (II), the n-hexane (poor solvent for PHEMA brush) was injected sequentially into the channel surface by micropump [34]. To verify the “tentacles” behavior, the samples containing ferritin were treated under a moist environment to remove the protein sheaths through bacterial digestion with Escherichia coli cells; the surface Fe cores aggregated without adhesion, whereas the ferritin units buried within the thin film were protected by the PHEMA brushes under these conditions. The elements of the surfaces were analyzed using XPS (Scientific Theta Probe, UK). The PHEMA brushes and the ferritin protein sheaths buried within them were then pyrolyzed under O2 in an oven at approximately 500 °C to reveal the ferrihydrite cores. The resolutions of various patterns of lines of PHEMA brushes after solvent treatment were measured using HR-SEM.Fig. 1


Ferritin immobilization on patterned poly(2-hydroxyethyl methacrylate) brushes on silicon surfaces from colloid system.

Chen TY, Chen JK - Colloid Polym Sci (2011)

Schematic representation of the strategy for ferritin capture. (I) The sample surface presenting the patterned PHEMA brushes is immersed for 1 h into a mixture of water and MeOH containing dispersed ferritin. (II) The sample surface is immersed into n-hexane to transform it from a brush-like to a mushroom-like structure, with the OH groups of the PHEMA brushes becoming buried within the PHEMA thin film to form hydrophilic domains. (III) The ferritin species on the PHEMA thin-film surface are removed through degradation of the protein sheath under O2 in an oven at approximately 500 °C to observe the ferrihydrite cores
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3040805&req=5

Fig1: Schematic representation of the strategy for ferritin capture. (I) The sample surface presenting the patterned PHEMA brushes is immersed for 1 h into a mixture of water and MeOH containing dispersed ferritin. (II) The sample surface is immersed into n-hexane to transform it from a brush-like to a mushroom-like structure, with the OH groups of the PHEMA brushes becoming buried within the PHEMA thin film to form hydrophilic domains. (III) The ferritin species on the PHEMA thin-film surface are removed through degradation of the protein sheath under O2 in an oven at approximately 500 °C to observe the ferrihydrite cores
Mentions: Ferritin (diameter of 13 nm; Fe core diameter of 7 nm; protein shell of 6 nm) was obtained from MP Biomedicals. Figure 1 displays the strategy used for ferritin capture. In Fig. 1 (I), the ferritin solution (good solvent for PHEMA brush) was injected into the channel surfaces patterned with PHEMA brushes by micropump. Ferritins adsorbed on the surface for all regions because of the protein shell of ferritins. In Fig. 1 (II), the n-hexane (poor solvent for PHEMA brush) was injected sequentially into the channel surface by micropump [34]. To verify the “tentacles” behavior, the samples containing ferritin were treated under a moist environment to remove the protein sheaths through bacterial digestion with Escherichia coli cells; the surface Fe cores aggregated without adhesion, whereas the ferritin units buried within the thin film were protected by the PHEMA brushes under these conditions. The elements of the surfaces were analyzed using XPS (Scientific Theta Probe, UK). The PHEMA brushes and the ferritin protein sheaths buried within them were then pyrolyzed under O2 in an oven at approximately 500 °C to reveal the ferrihydrite cores. The resolutions of various patterns of lines of PHEMA brushes after solvent treatment were measured using HR-SEM.Fig. 1

Bottom Line: The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system.The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system.Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, Taipei, 106 Taiwan Republic of China.

ABSTRACT
In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The "tentacles" behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the "tentacles" behaver for PHEMA brushes.

No MeSH data available.


Related in: MedlinePlus