The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I).
Bottom Line: Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding.Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response.In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process.
Affiliation: Institute of Immunology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.Show MeSH
Related in: MedlinePlus
Mentions: In order to evaluate moDCs for the presence of additional GP2 binding receptors, we investigated the contribution of SREC-I on the total GP2 binding. Therefore, we used anti-SREC-I antibodies to block the interaction of moDCs with GP2-bio. Ligation of SREC-I on SREC-I expressing Bw cells by specific antibodies resulted in a strong decrease of the MFI, indicating that the antibodies are suitable competitors of GP2 binding (Fig. 4A). In Fig. 4B expression of SREC-I on the surface of moDCs is demonstrated. By performing the inhibition experiments as shown in Fig. 4A for SREC-I expressing Bw cells, however, no decrease in GP2-bio binding to moDCs could be observed (Fig. 4C). This indicates the presence of additional GP2 binding structures on these cells.
Affiliation: Institute of Immunology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.