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The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I).

Hölzl MA, Hofer J, Kovarik JJ, Roggenbuck D, Reinhold D, Goihl A, Gärtner M, Steinberger P, Zlabinger GJ - Cell. Immunol. (2010)

Bottom Line: Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding.Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response.In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.

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Determination of the binding affinity of GP2 for SREC-I (A) Bw cells expressing SREC were incubated with indicated concentrations of GP2-bio and stained with SA-PE. (B) Inverted values of MFI and concentrations were used to create a Lineweaver–Burk diagram (right panel). Kd = −1/X(y0). MFI = Mean fluorescence intensity. Error bars indicate the standard error of the mean (n = 3).
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f0010: Determination of the binding affinity of GP2 for SREC-I (A) Bw cells expressing SREC were incubated with indicated concentrations of GP2-bio and stained with SA-PE. (B) Inverted values of MFI and concentrations were used to create a Lineweaver–Burk diagram (right panel). Kd = −1/X(y0). MFI = Mean fluorescence intensity. Error bars indicate the standard error of the mean (n = 3).

Mentions: Since GP2 clearly bound to cells expressing SREC-I, it was of interest to assess the affinity of this interaction. Therefore, SREC-I expressing cells were incubated with increasing amounts of GP2, resulting in an increased binding of the ligand (Fig. 2A).


The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I).

Hölzl MA, Hofer J, Kovarik JJ, Roggenbuck D, Reinhold D, Goihl A, Gärtner M, Steinberger P, Zlabinger GJ - Cell. Immunol. (2010)

Determination of the binding affinity of GP2 for SREC-I (A) Bw cells expressing SREC were incubated with indicated concentrations of GP2-bio and stained with SA-PE. (B) Inverted values of MFI and concentrations were used to create a Lineweaver–Burk diagram (right panel). Kd = −1/X(y0). MFI = Mean fluorescence intensity. Error bars indicate the standard error of the mean (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040788&req=5

f0010: Determination of the binding affinity of GP2 for SREC-I (A) Bw cells expressing SREC were incubated with indicated concentrations of GP2-bio and stained with SA-PE. (B) Inverted values of MFI and concentrations were used to create a Lineweaver–Burk diagram (right panel). Kd = −1/X(y0). MFI = Mean fluorescence intensity. Error bars indicate the standard error of the mean (n = 3).
Mentions: Since GP2 clearly bound to cells expressing SREC-I, it was of interest to assess the affinity of this interaction. Therefore, SREC-I expressing cells were incubated with increasing amounts of GP2, resulting in an increased binding of the ligand (Fig. 2A).

Bottom Line: Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding.Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response.In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process.

View Article: PubMed Central - PubMed

Affiliation: Institute of Immunology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.

Show MeSH
Related in: MedlinePlus