The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I).
Bottom Line: Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding.Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response.In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process.
Affiliation: Institute of Immunology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.Show MeSH
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Mentions: Retrovirally transduced Bw cells were evaluated for the expression of SRs by flow cytometry using SR specific antibodies. SR-AI, SR-BI and SREC-I were expressed on the surface of the respective Bw-clones at high levels (Fig. 1). To address whether SRs can bind their genuine ligand, SR expressing Bw clones were incubated with acLDL-488. All SR expressing clones but not mock transfected Bw cells strongly bound acLDL-488 (Fig. 1) and were therefore considered to be functional. Subsequently, SR expressing Bw clones were incubated with GP2-bio, which strongly bound to clones expressing SREC-I. No binding was observed with SR-AI or SR-BI or mock transfected Bw cells (Fig. 1). These results clearly identify SREC-I as a cellular receptor for GP2.
Affiliation: Institute of Immunology, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, A-1090 Vienna, Austria.