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A multi-functional synthetic gene network: a frequency multiplier, oscillator and switch.

Purcell O, di Bernardo M, Grierson CS, Savery NJ - PLoS ONE (2011)

Bottom Line: Analysis of the bifurcation structure also reveals novel, programmable multi-functionality; in addition to functioning as a frequency multiplier, the network is able to function as a switch or an oscillator, depending on the temporal nature of the input.Multi-functionality is often observed in neuronal networks, where it is suggested to allow for the efficient coordination of different responses.This network represents a significant theoretical addition that extends the capabilities of synthetic gene networks.

View Article: PubMed Central - PubMed

Affiliation: Department of Engineering Mathematics, Bristol Centre for Complexity Sciences, University of Bristol, Bristol, United Kingdom. enoep@bristol.ac.uk

ABSTRACT
We present the design and analysis of a synthetic gene network that performs frequency multiplication. It takes oscillatory transcription factor concentrations, such as those produced from the currently available genetic oscillators, as an input, and produces oscillations with half the input frequency as an output. Analysis of the bifurcation structure also reveals novel, programmable multi-functionality; in addition to functioning as a frequency multiplier, the network is able to function as a switch or an oscillator, depending on the temporal nature of the input. Multi-functionality is often observed in neuronal networks, where it is suggested to allow for the efficient coordination of different responses. This network represents a significant theoretical addition that extends the capabilities of synthetic gene networks.

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Possible in vivo implementation of the network.The repressors used are the set . The promoters used are: Fx e.g. F23 [21], P [35], pNOR [8] and P. Modified pNOR is the pNOR promoter with repression by LacI removed. The input to the network is arabinose + AraC, which form a complex that can activate transcription.
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pone-0016140-g009: Possible in vivo implementation of the network.The repressors used are the set . The promoters used are: Fx e.g. F23 [21], P [35], pNOR [8] and P. Modified pNOR is the pNOR promoter with repression by LacI removed. The input to the network is arabinose + AraC, which form a complex that can activate transcription.

Mentions: A possible implementation of the network is given in figure 9. It is based on constructing the network in E. coli, a tried and tested host for synthetic networks [4], [6], [9]–[11].


A multi-functional synthetic gene network: a frequency multiplier, oscillator and switch.

Purcell O, di Bernardo M, Grierson CS, Savery NJ - PLoS ONE (2011)

Possible in vivo implementation of the network.The repressors used are the set . The promoters used are: Fx e.g. F23 [21], P [35], pNOR [8] and P. Modified pNOR is the pNOR promoter with repression by LacI removed. The input to the network is arabinose + AraC, which form a complex that can activate transcription.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040778&req=5

pone-0016140-g009: Possible in vivo implementation of the network.The repressors used are the set . The promoters used are: Fx e.g. F23 [21], P [35], pNOR [8] and P. Modified pNOR is the pNOR promoter with repression by LacI removed. The input to the network is arabinose + AraC, which form a complex that can activate transcription.
Mentions: A possible implementation of the network is given in figure 9. It is based on constructing the network in E. coli, a tried and tested host for synthetic networks [4], [6], [9]–[11].

Bottom Line: Analysis of the bifurcation structure also reveals novel, programmable multi-functionality; in addition to functioning as a frequency multiplier, the network is able to function as a switch or an oscillator, depending on the temporal nature of the input.Multi-functionality is often observed in neuronal networks, where it is suggested to allow for the efficient coordination of different responses.This network represents a significant theoretical addition that extends the capabilities of synthetic gene networks.

View Article: PubMed Central - PubMed

Affiliation: Department of Engineering Mathematics, Bristol Centre for Complexity Sciences, University of Bristol, Bristol, United Kingdom. enoep@bristol.ac.uk

ABSTRACT
We present the design and analysis of a synthetic gene network that performs frequency multiplication. It takes oscillatory transcription factor concentrations, such as those produced from the currently available genetic oscillators, as an input, and produces oscillations with half the input frequency as an output. Analysis of the bifurcation structure also reveals novel, programmable multi-functionality; in addition to functioning as a frequency multiplier, the network is able to function as a switch or an oscillator, depending on the temporal nature of the input. Multi-functionality is often observed in neuronal networks, where it is suggested to allow for the efficient coordination of different responses. This network represents a significant theoretical addition that extends the capabilities of synthetic gene networks.

Show MeSH