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Adverse effect of nano-silicon dioxide on lung function of rats with or without ovalbumin immunization.

Han B, Guo J, Abrahaley T, Qin L, Wang L, Zheng Y, Li B, Liu D, Yao H, Yang J, Li C, Xi Z, Yang X - PLoS ONE (2011)

Bottom Line: Increased nano-SiO₂ exposure results in adverse changes on inspiratory and expiratory resistance (Ri and Re), but shows insignificant effect on rat lung dynamic compliance (Cldyn).The percentages of eosinophil display an unexpected result, in which higher exposure results lower eosinophil percentages.The results suggested that intratracheal administration of nano-SiO₂ could lead to the airway hyperresponsiveness (AHR) and the airway remolding with or without OVA immunization.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Environmental Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Huazhong Normal University, Wuhan, China.

ABSTRACT

Background: The great advances of nanomaterials have brought out broad important applications, but their possible nanotoxicity and risks have not been fully understood. It is confirmed that exposure of environmental particulate matter (PM), especially ultrafine PM, are responsible for many lung function impairment and exacerbation of pre-existing lung diseases. However, the adverse effect of nanoparticles on allergic asthma is seldom investigated and the mechanism remains undefined. For the first time, this work investigates the relationship between allergic asthma and nanosized silicon dioxide (nano-SiO₂).

Methodology/principal findings: Ovalbumin (OVA)-treated and saline-treated control rats were daily intratracheally administered 0.1 ml of 0, 40 and 80 µg/ml nano-SiO₂ solutions, respectively for 30 days. Increased nano-SiO₂ exposure results in adverse changes on inspiratory and expiratory resistance (Ri and Re), but shows insignificant effect on rat lung dynamic compliance (Cldyn). Lung histological observation reveals obvious airway remodeling in 80 µg/ml nano-SiO₂-introduced saline and OVA groups, but the latter is worse. Additionally, increased nano-SiO₂ exposure also leads to more severe inflammation. With increasing nano-SiO₂ exposure, IL-4 in lung homogenate increases and IFN-γ shows a reverse but insignificant change. Moreover, at a same nano-SiO₂ exposure concentration, OVA-treated rats exhibit higher (significant) IL-4 and lower (not significant) IFN-γ compared with the saline-treated rats. The percentages of eosinophil display an unexpected result, in which higher exposure results lower eosinophil percentages.

Conclusions/significance: This was a preliminary study which for the first time involved the effect of nano-SiO₂ to OVA induced rat asthma model. The results suggested that intratracheal administration of nano-SiO₂ could lead to the airway hyperresponsiveness (AHR) and the airway remolding with or without OVA immunization. This occurrence may be due to the Th1/Th2 cytokine imbalance accelerated by the nano-SiO₂ through increasing the tissue IL-4 production.

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Eosinophil percentages in BAL fluids ((Eosinophils/total cells) ×100%).(A) Saline treatment plus 0 µg/ml nano-SiO2 exposure. (B) Saline treatment plus 40 µg/ml nano-SiO2 exposure. (C) Saline treatment plus 80 µg/ml nano-SiO2 exposure. (D) OVA treatment plus 0 µg/ml nano-SiO2 exposure. (E) OVA treatment plus 40 µg/ml nano-SiO2 exposure. (F) OVA treatment plus 80 µg/ml nano-SiO2 exposure. # p<0.05, ## p<0.01, all compared with (D). ++ p<0.01, comparisons between the same exposure concentration groups.
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pone-0017236-g004: Eosinophil percentages in BAL fluids ((Eosinophils/total cells) ×100%).(A) Saline treatment plus 0 µg/ml nano-SiO2 exposure. (B) Saline treatment plus 40 µg/ml nano-SiO2 exposure. (C) Saline treatment plus 80 µg/ml nano-SiO2 exposure. (D) OVA treatment plus 0 µg/ml nano-SiO2 exposure. (E) OVA treatment plus 40 µg/ml nano-SiO2 exposure. (F) OVA treatment plus 80 µg/ml nano-SiO2 exposure. # p<0.05, ## p<0.01, all compared with (D). ++ p<0.01, comparisons between the same exposure concentration groups.

Mentions: The eosinophil percentages appeared to indicate an unexpected tendency of that a dose-dependent decline existed in the OVA groups when the exposure concentration increased (Fig. 4). 40 µg/ml nano-SiO2-introduced OVA group (group E) demonstrated significant decrease of eosinophil percentage (p<0.05) when compared with the OVA-alone group without the introduced nanoparticles (group D), and 80 µg/ml nano-SiO2-introduced OVA group (group F) was extremely lower (p<0.01) compared with that of the OVA-alone group (group D). The eosinophil percentages of the saline groups also showed decreased tendency, but not significant. Furthermore, the eosinophil percentage in the 80 µg/ml nano-SiO2-introduced OVA group (group F) was extremely lower than that of 80 µg/ml nano-SiO2-introduced saline group (group C).


Adverse effect of nano-silicon dioxide on lung function of rats with or without ovalbumin immunization.

Han B, Guo J, Abrahaley T, Qin L, Wang L, Zheng Y, Li B, Liu D, Yao H, Yang J, Li C, Xi Z, Yang X - PLoS ONE (2011)

Eosinophil percentages in BAL fluids ((Eosinophils/total cells) ×100%).(A) Saline treatment plus 0 µg/ml nano-SiO2 exposure. (B) Saline treatment plus 40 µg/ml nano-SiO2 exposure. (C) Saline treatment plus 80 µg/ml nano-SiO2 exposure. (D) OVA treatment plus 0 µg/ml nano-SiO2 exposure. (E) OVA treatment plus 40 µg/ml nano-SiO2 exposure. (F) OVA treatment plus 80 µg/ml nano-SiO2 exposure. # p<0.05, ## p<0.01, all compared with (D). ++ p<0.01, comparisons between the same exposure concentration groups.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040772&req=5

pone-0017236-g004: Eosinophil percentages in BAL fluids ((Eosinophils/total cells) ×100%).(A) Saline treatment plus 0 µg/ml nano-SiO2 exposure. (B) Saline treatment plus 40 µg/ml nano-SiO2 exposure. (C) Saline treatment plus 80 µg/ml nano-SiO2 exposure. (D) OVA treatment plus 0 µg/ml nano-SiO2 exposure. (E) OVA treatment plus 40 µg/ml nano-SiO2 exposure. (F) OVA treatment plus 80 µg/ml nano-SiO2 exposure. # p<0.05, ## p<0.01, all compared with (D). ++ p<0.01, comparisons between the same exposure concentration groups.
Mentions: The eosinophil percentages appeared to indicate an unexpected tendency of that a dose-dependent decline existed in the OVA groups when the exposure concentration increased (Fig. 4). 40 µg/ml nano-SiO2-introduced OVA group (group E) demonstrated significant decrease of eosinophil percentage (p<0.05) when compared with the OVA-alone group without the introduced nanoparticles (group D), and 80 µg/ml nano-SiO2-introduced OVA group (group F) was extremely lower (p<0.01) compared with that of the OVA-alone group (group D). The eosinophil percentages of the saline groups also showed decreased tendency, but not significant. Furthermore, the eosinophil percentage in the 80 µg/ml nano-SiO2-introduced OVA group (group F) was extremely lower than that of 80 µg/ml nano-SiO2-introduced saline group (group C).

Bottom Line: Increased nano-SiO₂ exposure results in adverse changes on inspiratory and expiratory resistance (Ri and Re), but shows insignificant effect on rat lung dynamic compliance (Cldyn).The percentages of eosinophil display an unexpected result, in which higher exposure results lower eosinophil percentages.The results suggested that intratracheal administration of nano-SiO₂ could lead to the airway hyperresponsiveness (AHR) and the airway remolding with or without OVA immunization.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Environmental Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Huazhong Normal University, Wuhan, China.

ABSTRACT

Background: The great advances of nanomaterials have brought out broad important applications, but their possible nanotoxicity and risks have not been fully understood. It is confirmed that exposure of environmental particulate matter (PM), especially ultrafine PM, are responsible for many lung function impairment and exacerbation of pre-existing lung diseases. However, the adverse effect of nanoparticles on allergic asthma is seldom investigated and the mechanism remains undefined. For the first time, this work investigates the relationship between allergic asthma and nanosized silicon dioxide (nano-SiO₂).

Methodology/principal findings: Ovalbumin (OVA)-treated and saline-treated control rats were daily intratracheally administered 0.1 ml of 0, 40 and 80 µg/ml nano-SiO₂ solutions, respectively for 30 days. Increased nano-SiO₂ exposure results in adverse changes on inspiratory and expiratory resistance (Ri and Re), but shows insignificant effect on rat lung dynamic compliance (Cldyn). Lung histological observation reveals obvious airway remodeling in 80 µg/ml nano-SiO₂-introduced saline and OVA groups, but the latter is worse. Additionally, increased nano-SiO₂ exposure also leads to more severe inflammation. With increasing nano-SiO₂ exposure, IL-4 in lung homogenate increases and IFN-γ shows a reverse but insignificant change. Moreover, at a same nano-SiO₂ exposure concentration, OVA-treated rats exhibit higher (significant) IL-4 and lower (not significant) IFN-γ compared with the saline-treated rats. The percentages of eosinophil display an unexpected result, in which higher exposure results lower eosinophil percentages.

Conclusions/significance: This was a preliminary study which for the first time involved the effect of nano-SiO₂ to OVA induced rat asthma model. The results suggested that intratracheal administration of nano-SiO₂ could lead to the airway hyperresponsiveness (AHR) and the airway remolding with or without OVA immunization. This occurrence may be due to the Th1/Th2 cytokine imbalance accelerated by the nano-SiO₂ through increasing the tissue IL-4 production.

Show MeSH
Related in: MedlinePlus