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Overexpression of nuclear protein kinase CK2 α catalytic subunit (CK2α) as a poor prognosticator in human colorectal cancer.

Lin KY, Tai C, Hsu JC, Li CF, Fang CL, Lai HC, Hseu YC, Lin YF, Uen YH - PLoS ONE (2011)

Bottom Line: Thus, novel therapeutic approaches are badly needed.We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients.The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.

ABSTRACT

Background: Colorectal cancer (CRC) is one of the most common malignancies but the current therapeutic approaches for advanced CRC are less efficient. Thus, novel therapeutic approaches are badly needed. The purpose of this study is to investigate the involvement of nuclear protein kinase CK2 α subunit (CK2α) in tumor progression, and in the prognosis of human CRC.

Methodology/principal findings: Expression levels of nuclear CK2α were analyzed in 245 colorectal tissues from patients with CRC by immunohistochemistry, quantitative real-time PCR and Western blot. We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients. Overexpression of nuclear CK2α was significantly correlated with depth of invasion, nodal status, American Joint Committee on Cancer (AJCC) staging, degree of differentiation, and perineural invasion. Patients with high expression levels of nuclear CK2α had a significantly poorer overall survival rate compared with patients with low expression levels of nuclear CK2α. In multi-variate Cox regression analysis, overexpression of nuclear CK2α was proven to be an independent prognostic marker for CRC. In addition, DLD-1 human colon cancer cells were employed as a cellular model to study the role of CK2α on cell growth, and the expression of CK2α in DLD-1 cells was inhibited by using siRNA technology. The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

Conclusions/significance: Taken together, overexpression of nuclear CK2α can be a useful marker for predicting the outcome of patients with CRC.

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Related in: MedlinePlus

Overall survival analysis of 245 CRC patients stratified by nuclear                            CK2α immunoreactivity (low nuclear CK2α: mean labeling index                            ≤40%; high nuclear CK2α: mean labeling index                            >40%).All statistical tests were two-sided. Significance level:                                P<0.05.
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pone-0017193-g003: Overall survival analysis of 245 CRC patients stratified by nuclear CK2α immunoreactivity (low nuclear CK2α: mean labeling index ≤40%; high nuclear CK2α: mean labeling index >40%).All statistical tests were two-sided. Significance level: P<0.05.

Mentions: Correlations of clinical outcomes with nuclear CK2α expression are shown in Figure 3. Inferior overall survival was significantly associated with overexpression of nuclear CK2α (mean labeling index >40%, P<0.0001). Patients with high expression levels of nuclear CK2α had a ten-year overall survival rate of 22.2% compared with 72.5% for patients with low expression levels of nuclear CK2α.


Overexpression of nuclear protein kinase CK2 α catalytic subunit (CK2α) as a poor prognosticator in human colorectal cancer.

Lin KY, Tai C, Hsu JC, Li CF, Fang CL, Lai HC, Hseu YC, Lin YF, Uen YH - PLoS ONE (2011)

Overall survival analysis of 245 CRC patients stratified by nuclear                            CK2α immunoreactivity (low nuclear CK2α: mean labeling index                            ≤40%; high nuclear CK2α: mean labeling index                            >40%).All statistical tests were two-sided. Significance level:                                P<0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040762&req=5

pone-0017193-g003: Overall survival analysis of 245 CRC patients stratified by nuclear CK2α immunoreactivity (low nuclear CK2α: mean labeling index ≤40%; high nuclear CK2α: mean labeling index >40%).All statistical tests were two-sided. Significance level: P<0.05.
Mentions: Correlations of clinical outcomes with nuclear CK2α expression are shown in Figure 3. Inferior overall survival was significantly associated with overexpression of nuclear CK2α (mean labeling index >40%, P<0.0001). Patients with high expression levels of nuclear CK2α had a ten-year overall survival rate of 22.2% compared with 72.5% for patients with low expression levels of nuclear CK2α.

Bottom Line: Thus, novel therapeutic approaches are badly needed.We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients.The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.

ABSTRACT

Background: Colorectal cancer (CRC) is one of the most common malignancies but the current therapeutic approaches for advanced CRC are less efficient. Thus, novel therapeutic approaches are badly needed. The purpose of this study is to investigate the involvement of nuclear protein kinase CK2 α subunit (CK2α) in tumor progression, and in the prognosis of human CRC.

Methodology/principal findings: Expression levels of nuclear CK2α were analyzed in 245 colorectal tissues from patients with CRC by immunohistochemistry, quantitative real-time PCR and Western blot. We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients. Overexpression of nuclear CK2α was significantly correlated with depth of invasion, nodal status, American Joint Committee on Cancer (AJCC) staging, degree of differentiation, and perineural invasion. Patients with high expression levels of nuclear CK2α had a significantly poorer overall survival rate compared with patients with low expression levels of nuclear CK2α. In multi-variate Cox regression analysis, overexpression of nuclear CK2α was proven to be an independent prognostic marker for CRC. In addition, DLD-1 human colon cancer cells were employed as a cellular model to study the role of CK2α on cell growth, and the expression of CK2α in DLD-1 cells was inhibited by using siRNA technology. The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

Conclusions/significance: Taken together, overexpression of nuclear CK2α can be a useful marker for predicting the outcome of patients with CRC.

Show MeSH
Related in: MedlinePlus