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Overexpression of nuclear protein kinase CK2 α catalytic subunit (CK2α) as a poor prognosticator in human colorectal cancer.

Lin KY, Tai C, Hsu JC, Li CF, Fang CL, Lai HC, Hseu YC, Lin YF, Uen YH - PLoS ONE (2011)

Bottom Line: Thus, novel therapeutic approaches are badly needed.We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients.The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.

ABSTRACT

Background: Colorectal cancer (CRC) is one of the most common malignancies but the current therapeutic approaches for advanced CRC are less efficient. Thus, novel therapeutic approaches are badly needed. The purpose of this study is to investigate the involvement of nuclear protein kinase CK2 α subunit (CK2α) in tumor progression, and in the prognosis of human CRC.

Methodology/principal findings: Expression levels of nuclear CK2α were analyzed in 245 colorectal tissues from patients with CRC by immunohistochemistry, quantitative real-time PCR and Western blot. We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients. Overexpression of nuclear CK2α was significantly correlated with depth of invasion, nodal status, American Joint Committee on Cancer (AJCC) staging, degree of differentiation, and perineural invasion. Patients with high expression levels of nuclear CK2α had a significantly poorer overall survival rate compared with patients with low expression levels of nuclear CK2α. In multi-variate Cox regression analysis, overexpression of nuclear CK2α was proven to be an independent prognostic marker for CRC. In addition, DLD-1 human colon cancer cells were employed as a cellular model to study the role of CK2α on cell growth, and the expression of CK2α in DLD-1 cells was inhibited by using siRNA technology. The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

Conclusions/significance: Taken together, overexpression of nuclear CK2α can be a useful marker for predicting the outcome of patients with CRC.

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Related in: MedlinePlus

The representative IHC nuclear CK2α staining corresponding to                            mean nuclear CK2α labeling index values for different                            parameters.Magnification: 400×.
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pone-0017193-g002: The representative IHC nuclear CK2α staining corresponding to mean nuclear CK2α labeling index values for different parameters.Magnification: 400×.

Mentions: We investigated the expression of nuclear CK2α in 245 CRC patients by immunohistochemistry, and in four patients by Western blot. The results indicated that nuclear CK2α expression was higher in tumor tissues than in non-tumor tissues (Figure 1). Additionally, quantitative real-time PCR analysis demonstrated that the expression of CK2α was substantially increased in tumor tissues when compared with non-tumor tissues (Table 1). As shown in Table 2, nuclear CK2α expression had a statistically significant correlation with depth of invasion (P = 0.008), nodal status (P<0.001), AJCC staging (P<0.001), degree of differentiation (P = 0.011), and perineural invasion (P = 0.041). The representative photomicrographs of nuclear CK2α expressions for different parameters were shown in Figure 2. There was no significant association between nuclear CK2α expression and age, gender, and vascular invasion.


Overexpression of nuclear protein kinase CK2 α catalytic subunit (CK2α) as a poor prognosticator in human colorectal cancer.

Lin KY, Tai C, Hsu JC, Li CF, Fang CL, Lai HC, Hseu YC, Lin YF, Uen YH - PLoS ONE (2011)

The representative IHC nuclear CK2α staining corresponding to                            mean nuclear CK2α labeling index values for different                            parameters.Magnification: 400×.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040762&req=5

pone-0017193-g002: The representative IHC nuclear CK2α staining corresponding to mean nuclear CK2α labeling index values for different parameters.Magnification: 400×.
Mentions: We investigated the expression of nuclear CK2α in 245 CRC patients by immunohistochemistry, and in four patients by Western blot. The results indicated that nuclear CK2α expression was higher in tumor tissues than in non-tumor tissues (Figure 1). Additionally, quantitative real-time PCR analysis demonstrated that the expression of CK2α was substantially increased in tumor tissues when compared with non-tumor tissues (Table 1). As shown in Table 2, nuclear CK2α expression had a statistically significant correlation with depth of invasion (P = 0.008), nodal status (P<0.001), AJCC staging (P<0.001), degree of differentiation (P = 0.011), and perineural invasion (P = 0.041). The representative photomicrographs of nuclear CK2α expressions for different parameters were shown in Figure 2. There was no significant association between nuclear CK2α expression and age, gender, and vascular invasion.

Bottom Line: Thus, novel therapeutic approaches are badly needed.We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients.The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.

ABSTRACT

Background: Colorectal cancer (CRC) is one of the most common malignancies but the current therapeutic approaches for advanced CRC are less efficient. Thus, novel therapeutic approaches are badly needed. The purpose of this study is to investigate the involvement of nuclear protein kinase CK2 α subunit (CK2α) in tumor progression, and in the prognosis of human CRC.

Methodology/principal findings: Expression levels of nuclear CK2α were analyzed in 245 colorectal tissues from patients with CRC by immunohistochemistry, quantitative real-time PCR and Western blot. We correlated the expression levels with clinicopathologic parameters and prognosis in human CRC patients. Overexpression of nuclear CK2α was significantly correlated with depth of invasion, nodal status, American Joint Committee on Cancer (AJCC) staging, degree of differentiation, and perineural invasion. Patients with high expression levels of nuclear CK2α had a significantly poorer overall survival rate compared with patients with low expression levels of nuclear CK2α. In multi-variate Cox regression analysis, overexpression of nuclear CK2α was proven to be an independent prognostic marker for CRC. In addition, DLD-1 human colon cancer cells were employed as a cellular model to study the role of CK2α on cell growth, and the expression of CK2α in DLD-1 cells was inhibited by using siRNA technology. The data indicated that CK2α-specific siRNA treatment resulted in growth inhibition.

Conclusions/significance: Taken together, overexpression of nuclear CK2α can be a useful marker for predicting the outcome of patients with CRC.

Show MeSH
Related in: MedlinePlus