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Quantitative 3-dimensional imaging of murine neointimal and atherosclerotic lesions by optical projection tomography.

Kirkby NS, Low L, Seckl JR, Walker BR, Webb DJ, Hadoke PW - PLoS ONE (2011)

Bottom Line: Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature.It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, United Kingdom.

ABSTRACT

Objective: Traditional methods for the analysis of vascular lesion formation are labour intensive to perform - restricting study to 'snapshots' within each vessel. This study was undertaken to determine the suitability of optical projection tomographic (OPT) imaging for the 3-dimensional representation and quantification of intimal lesions in mouse arteries.

Methods and results: Vascular injury was induced by wire-insertion or ligation of the mouse femoral artery or administration of an atherogenic diet to apoE-deficient mice. Lesion formation was examined by OPT imaging of autofluorescent emission. Lesions could be clearly identified and distinguished from the underlying vascular wall. Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature. It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).

Conclusions: These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

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Related in: MedlinePlus

Identification of atherosclerotic lesion formation in the aortic arch of apoE-/- mice.In non-tomographic fluorescent emission images of the aortic arch of atherosclerotic mice (a), lesion formation (red arrowheads) is apparent with the expected distribution. Image has been inverted for clarity (darker regions reflect stronger emission). In cross-sectional reconstructions, lesions are visible with the same distribution (b) and possess strong resemblance to histological sections of the same vessels (c). Successful analysis of the brachiocephalic artery by standard (immuno)-histochemical procedures further emphasises the non-destructive nature of this technique (c). Scale bars in (a–b) are 1 mm. RSA, right subclavian artery; RCA, right carotid artery; LCA, left carotid artery; LSA, left subclavian artery; BCA, brachiocephalic artery; AAo, ascending aorta; DAo, descending aorta.
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pone-0016906-g002: Identification of atherosclerotic lesion formation in the aortic arch of apoE-/- mice.In non-tomographic fluorescent emission images of the aortic arch of atherosclerotic mice (a), lesion formation (red arrowheads) is apparent with the expected distribution. Image has been inverted for clarity (darker regions reflect stronger emission). In cross-sectional reconstructions, lesions are visible with the same distribution (b) and possess strong resemblance to histological sections of the same vessels (c). Successful analysis of the brachiocephalic artery by standard (immuno)-histochemical procedures further emphasises the non-destructive nature of this technique (c). Scale bars in (a–b) are 1 mm. RSA, right subclavian artery; RCA, right carotid artery; LCA, left carotid artery; LSA, left subclavian artery; BCA, brachiocephalic artery; AAo, ascending aorta; DAo, descending aorta.

Mentions: To extend the possible application of this technique to atherosclerotic lesions in large conduit arteries, the aortic arch from apoE-/- mice was also examined (n = 8). In OPT emission images of atherosclerotic aortic arches, lesion formation was again clearly evident with the expected anatomical distribution comprising lesions in the lesser curvature of the arch, in the brachiocephalic artery and at the origins of the left carotid and left subclavian arteries (Figure 2a). In cross-section, these lesions typically possessed an eccentric morphology and could be distinguished from media and lumen (Figure 2b,c, Figure S2).


Quantitative 3-dimensional imaging of murine neointimal and atherosclerotic lesions by optical projection tomography.

Kirkby NS, Low L, Seckl JR, Walker BR, Webb DJ, Hadoke PW - PLoS ONE (2011)

Identification of atherosclerotic lesion formation in the aortic arch of apoE-/- mice.In non-tomographic fluorescent emission images of the aortic arch of atherosclerotic mice (a), lesion formation (red arrowheads) is apparent with the expected distribution. Image has been inverted for clarity (darker regions reflect stronger emission). In cross-sectional reconstructions, lesions are visible with the same distribution (b) and possess strong resemblance to histological sections of the same vessels (c). Successful analysis of the brachiocephalic artery by standard (immuno)-histochemical procedures further emphasises the non-destructive nature of this technique (c). Scale bars in (a–b) are 1 mm. RSA, right subclavian artery; RCA, right carotid artery; LCA, left carotid artery; LSA, left subclavian artery; BCA, brachiocephalic artery; AAo, ascending aorta; DAo, descending aorta.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040742&req=5

pone-0016906-g002: Identification of atherosclerotic lesion formation in the aortic arch of apoE-/- mice.In non-tomographic fluorescent emission images of the aortic arch of atherosclerotic mice (a), lesion formation (red arrowheads) is apparent with the expected distribution. Image has been inverted for clarity (darker regions reflect stronger emission). In cross-sectional reconstructions, lesions are visible with the same distribution (b) and possess strong resemblance to histological sections of the same vessels (c). Successful analysis of the brachiocephalic artery by standard (immuno)-histochemical procedures further emphasises the non-destructive nature of this technique (c). Scale bars in (a–b) are 1 mm. RSA, right subclavian artery; RCA, right carotid artery; LCA, left carotid artery; LSA, left subclavian artery; BCA, brachiocephalic artery; AAo, ascending aorta; DAo, descending aorta.
Mentions: To extend the possible application of this technique to atherosclerotic lesions in large conduit arteries, the aortic arch from apoE-/- mice was also examined (n = 8). In OPT emission images of atherosclerotic aortic arches, lesion formation was again clearly evident with the expected anatomical distribution comprising lesions in the lesser curvature of the arch, in the brachiocephalic artery and at the origins of the left carotid and left subclavian arteries (Figure 2a). In cross-section, these lesions typically possessed an eccentric morphology and could be distinguished from media and lumen (Figure 2b,c, Figure S2).

Bottom Line: Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature.It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, United Kingdom.

ABSTRACT

Objective: Traditional methods for the analysis of vascular lesion formation are labour intensive to perform - restricting study to 'snapshots' within each vessel. This study was undertaken to determine the suitability of optical projection tomographic (OPT) imaging for the 3-dimensional representation and quantification of intimal lesions in mouse arteries.

Methods and results: Vascular injury was induced by wire-insertion or ligation of the mouse femoral artery or administration of an atherogenic diet to apoE-deficient mice. Lesion formation was examined by OPT imaging of autofluorescent emission. Lesions could be clearly identified and distinguished from the underlying vascular wall. Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature. It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).

Conclusions: These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

Show MeSH
Related in: MedlinePlus