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Quantitative 3-dimensional imaging of murine neointimal and atherosclerotic lesions by optical projection tomography.

Kirkby NS, Low L, Seckl JR, Walker BR, Webb DJ, Hadoke PW - PLoS ONE (2011)

Bottom Line: Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature.It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, United Kingdom.

ABSTRACT

Objective: Traditional methods for the analysis of vascular lesion formation are labour intensive to perform - restricting study to 'snapshots' within each vessel. This study was undertaken to determine the suitability of optical projection tomographic (OPT) imaging for the 3-dimensional representation and quantification of intimal lesions in mouse arteries.

Methods and results: Vascular injury was induced by wire-insertion or ligation of the mouse femoral artery or administration of an atherogenic diet to apoE-deficient mice. Lesion formation was examined by OPT imaging of autofluorescent emission. Lesions could be clearly identified and distinguished from the underlying vascular wall. Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature. It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).

Conclusions: These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

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Related in: MedlinePlus

Identification of neointimal lesions in the ligation-injured mouse femoral artery.In non-tomographic fluorescent emission images of a ligation-injured femoral artery (a) neointimal thickening is clearly visible (red arrowheads). Image has been inverted for clarity (darker regions reflect stronger emission). In tomographic reconstructions (b), all layers of the vessel wall can be identified. Reconstructions strongly resemble US trichrome-stained histological sections of the same vessels (c). Scale bars in (a–c) are 200 µm.
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pone-0016906-g001: Identification of neointimal lesions in the ligation-injured mouse femoral artery.In non-tomographic fluorescent emission images of a ligation-injured femoral artery (a) neointimal thickening is clearly visible (red arrowheads). Image has been inverted for clarity (darker regions reflect stronger emission). In tomographic reconstructions (b), all layers of the vessel wall can be identified. Reconstructions strongly resemble US trichrome-stained histological sections of the same vessels (c). Scale bars in (a–c) are 200 µm.

Mentions: To establish whether OPT imaging could characterise neointimal lesion formation, mouse femoral arteries were subject to wire- (n = 6) or ligation- (n = 5) injury. Following either insult, neointimal thickening could be seen clearly in non-tomographic emission projections (Figure 1a). In reconstructed 2-dimensional slices, concentric neointimal lesions were obvious and could be distinguished from the media by their weaker emission (Figure 1b, Figure S1).


Quantitative 3-dimensional imaging of murine neointimal and atherosclerotic lesions by optical projection tomography.

Kirkby NS, Low L, Seckl JR, Walker BR, Webb DJ, Hadoke PW - PLoS ONE (2011)

Identification of neointimal lesions in the ligation-injured mouse femoral artery.In non-tomographic fluorescent emission images of a ligation-injured femoral artery (a) neointimal thickening is clearly visible (red arrowheads). Image has been inverted for clarity (darker regions reflect stronger emission). In tomographic reconstructions (b), all layers of the vessel wall can be identified. Reconstructions strongly resemble US trichrome-stained histological sections of the same vessels (c). Scale bars in (a–c) are 200 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040742&req=5

pone-0016906-g001: Identification of neointimal lesions in the ligation-injured mouse femoral artery.In non-tomographic fluorescent emission images of a ligation-injured femoral artery (a) neointimal thickening is clearly visible (red arrowheads). Image has been inverted for clarity (darker regions reflect stronger emission). In tomographic reconstructions (b), all layers of the vessel wall can be identified. Reconstructions strongly resemble US trichrome-stained histological sections of the same vessels (c). Scale bars in (a–c) are 200 µm.
Mentions: To establish whether OPT imaging could characterise neointimal lesion formation, mouse femoral arteries were subject to wire- (n = 6) or ligation- (n = 5) injury. Following either insult, neointimal thickening could be seen clearly in non-tomographic emission projections (Figure 1a). In reconstructed 2-dimensional slices, concentric neointimal lesions were obvious and could be distinguished from the media by their weaker emission (Figure 1b, Figure S1).

Bottom Line: Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature.It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, United Kingdom.

ABSTRACT

Objective: Traditional methods for the analysis of vascular lesion formation are labour intensive to perform - restricting study to 'snapshots' within each vessel. This study was undertaken to determine the suitability of optical projection tomographic (OPT) imaging for the 3-dimensional representation and quantification of intimal lesions in mouse arteries.

Methods and results: Vascular injury was induced by wire-insertion or ligation of the mouse femoral artery or administration of an atherogenic diet to apoE-deficient mice. Lesion formation was examined by OPT imaging of autofluorescent emission. Lesions could be clearly identified and distinguished from the underlying vascular wall. Planimetric measurements of lesion area correlated well with those made from histological sections subsequently produced from the same vessels (wire-injury: R²  =  0.92; ligation-injury: R²  =  0.89; atherosclerosis: R²  =  0.85), confirming both the accuracy of this methodology and its non-destructive nature. It was also possible to record volumetric measurements of lesion and lumen and these were highly reproducible between scans (coefficient of variation  =  5.36%, 11.39% and 4.79% for wire- and ligation-injury and atherosclerosis, respectively).

Conclusions: These data demonstrate the eminent suitability of OPT for imaging of atherosclerotic and neointimal lesion formation, providing a much needed means for the routine 3-dimensional analysis of vascular morphology in studies of this type.

Show MeSH
Related in: MedlinePlus