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E. coli Nissle 1917 Affects Salmonella adhesion to porcine intestinal epithelial cells.

Schierack P, Kleta S, Tedin K, Babila JT, Oswald S, Oelschlaeger TA, Hiemann R, Paetzold S, Wieler LH - PLoS ONE (2011)

Bottom Line: Another E. coli strain expressing F1C fimbriae was also adherent to IPEC-J2 cells, and was similarly inhibitory against Salmonella invasion like EcN.We propose that EcN affects Salmonella adhesion through secretory components.This mechanism appears to be common to many E. coli strains, with strong adherence being a prerequisite for an effective reduction of SiiE-mediated Salmonella adhesion.

View Article: PubMed Central - PubMed

Affiliation: Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, Berlin, Germany. Peter.Schierack@HS-Lausitz.de

ABSTRACT

Background: The probiotic Escherichia coli strain Nissle 1917 (EcN) has been shown to interfere in a human in vitro model with the invasion of several bacterial pathogens into epithelial cells, but the underlying molecular mechanisms are not known.

Methodology/principal findings: In this study, we investigated the inhibitory effects of EcN on Salmonella Typhimurium invasion of porcine intestinal epithelial cells, focusing on EcN effects on the various stages of Salmonella infection including intracellular and extracellular Salmonella growth rates, virulence gene regulation, and adhesion. We show that EcN affects the initial Salmonella invasion steps by modulating Salmonella virulence gene regulation and Salmonella SiiE-mediated adhesion, but not extra- and intracellular Salmonella growth. However, the inhibitory activity of EcN against Salmonella invasion always correlated with EcN adhesion capacities. EcN mutants defective in the expression of F1C fimbriae and flagellae were less adherent and less inhibitory toward Salmonella invasion. Another E. coli strain expressing F1C fimbriae was also adherent to IPEC-J2 cells, and was similarly inhibitory against Salmonella invasion like EcN.

Conclusions: We propose that EcN affects Salmonella adhesion through secretory components. This mechanism appears to be common to many E. coli strains, with strong adherence being a prerequisite for an effective reduction of SiiE-mediated Salmonella adhesion.

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Related in: MedlinePlus

Invasion efficiency of Salmonella Typhimurium into IPEC-J2 cells after pre-incubation with E. coli mixed cultures.Confluent monolayers of IPEC-J2 cells were pre-incubated with E. coli Nissle 1917 (EcN) monocultures or mixed cultures using an MOI of 100∶1 or 10∶1 E. coli to host cells. After two hours, cells were washed and infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells. Invasion levels in percent (%) are expressed as invasion of Salmonella relative to invasion without pre-incubation with E. coli (Salmonella mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to Salmonella mono-infection.
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pone-0014712-g003: Invasion efficiency of Salmonella Typhimurium into IPEC-J2 cells after pre-incubation with E. coli mixed cultures.Confluent monolayers of IPEC-J2 cells were pre-incubated with E. coli Nissle 1917 (EcN) monocultures or mixed cultures using an MOI of 100∶1 or 10∶1 E. coli to host cells. After two hours, cells were washed and infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells. Invasion levels in percent (%) are expressed as invasion of Salmonella relative to invasion without pre-incubation with E. coli (Salmonella mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to Salmonella mono-infection.

Mentions: Inhibition of Salmonella invasion by EcN was also observed in mixed E. coli cultures although the effects on invasion were less effective in mixed E. coli cultures compared to EcN in the monoculture model. Using a mixture of EcN:E. coli 140815:E. coli MG1655 (1∶1∶1), at a total MOI of 10∶1 the inhibitory effects of EcN were abolished (Figure 3).


E. coli Nissle 1917 Affects Salmonella adhesion to porcine intestinal epithelial cells.

Schierack P, Kleta S, Tedin K, Babila JT, Oswald S, Oelschlaeger TA, Hiemann R, Paetzold S, Wieler LH - PLoS ONE (2011)

Invasion efficiency of Salmonella Typhimurium into IPEC-J2 cells after pre-incubation with E. coli mixed cultures.Confluent monolayers of IPEC-J2 cells were pre-incubated with E. coli Nissle 1917 (EcN) monocultures or mixed cultures using an MOI of 100∶1 or 10∶1 E. coli to host cells. After two hours, cells were washed and infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells. Invasion levels in percent (%) are expressed as invasion of Salmonella relative to invasion without pre-incubation with E. coli (Salmonella mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to Salmonella mono-infection.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3040738&req=5

pone-0014712-g003: Invasion efficiency of Salmonella Typhimurium into IPEC-J2 cells after pre-incubation with E. coli mixed cultures.Confluent monolayers of IPEC-J2 cells were pre-incubated with E. coli Nissle 1917 (EcN) monocultures or mixed cultures using an MOI of 100∶1 or 10∶1 E. coli to host cells. After two hours, cells were washed and infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells. Invasion levels in percent (%) are expressed as invasion of Salmonella relative to invasion without pre-incubation with E. coli (Salmonella mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to Salmonella mono-infection.
Mentions: Inhibition of Salmonella invasion by EcN was also observed in mixed E. coli cultures although the effects on invasion were less effective in mixed E. coli cultures compared to EcN in the monoculture model. Using a mixture of EcN:E. coli 140815:E. coli MG1655 (1∶1∶1), at a total MOI of 10∶1 the inhibitory effects of EcN were abolished (Figure 3).

Bottom Line: Another E. coli strain expressing F1C fimbriae was also adherent to IPEC-J2 cells, and was similarly inhibitory against Salmonella invasion like EcN.We propose that EcN affects Salmonella adhesion through secretory components.This mechanism appears to be common to many E. coli strains, with strong adherence being a prerequisite for an effective reduction of SiiE-mediated Salmonella adhesion.

View Article: PubMed Central - PubMed

Affiliation: Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, Berlin, Germany. Peter.Schierack@HS-Lausitz.de

ABSTRACT

Background: The probiotic Escherichia coli strain Nissle 1917 (EcN) has been shown to interfere in a human in vitro model with the invasion of several bacterial pathogens into epithelial cells, but the underlying molecular mechanisms are not known.

Methodology/principal findings: In this study, we investigated the inhibitory effects of EcN on Salmonella Typhimurium invasion of porcine intestinal epithelial cells, focusing on EcN effects on the various stages of Salmonella infection including intracellular and extracellular Salmonella growth rates, virulence gene regulation, and adhesion. We show that EcN affects the initial Salmonella invasion steps by modulating Salmonella virulence gene regulation and Salmonella SiiE-mediated adhesion, but not extra- and intracellular Salmonella growth. However, the inhibitory activity of EcN against Salmonella invasion always correlated with EcN adhesion capacities. EcN mutants defective in the expression of F1C fimbriae and flagellae were less adherent and less inhibitory toward Salmonella invasion. Another E. coli strain expressing F1C fimbriae was also adherent to IPEC-J2 cells, and was similarly inhibitory against Salmonella invasion like EcN.

Conclusions: We propose that EcN affects Salmonella adhesion through secretory components. This mechanism appears to be common to many E. coli strains, with strong adherence being a prerequisite for an effective reduction of SiiE-mediated Salmonella adhesion.

Show MeSH
Related in: MedlinePlus