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Fine-tuning the space, time, and host distribution of mycobacteria in wildlife.

Gortazar C, Torres MJ, Acevedo P, Aznar J, Negro JJ, de la Fuente J, Vicente J - BMC Microbiol. (2011)

Bottom Line: While only three TPs were detected in wildlife between 1998 and 2003, up to 8 different ones were found during 2006-2007.M. bovis TPs were usually found closer to water marshland than MOTT.This research highlights the suitability of molecular typing for surveys at small spatial and temporal scales.

View Article: PubMed Central - HTML - PubMed

Affiliation: IREC National Wildlife Research Institute (CSIC-UCLM-JCCM), Ciudad Real, Spain.

ABSTRACT

Background: We describe the diversity of two kinds of mycobacteria isolates, environmental mycobacteria and Mycobacterium bovis collected from wild boar, fallow deer, red deer and cattle in Doñana National Park (DNP, Spain), analyzing their association with temporal, spatial and environmental factors.

Results: High diversity of environmental mycobacteria species and M. bovis typing patterns (TPs) were found. When assessing the factors underlying the presence of the most common types of both environmental mycobacteria and M. bovis TPs in DNP, we evidenced (i) host species differences in the occurrence, (ii) spatial structuration and (iii) differences in the degree of spatial association of specific types between host species. Co-infection of a single host by two M. bovis TPs occurred in all three wild ungulate species. In wild boar and red deer, isolation of one group of mycobacteria occurred more frequently in individuals not infected by the other group. While only three TPs were detected in wildlife between 1998 and 2003, up to 8 different ones were found during 2006-2007. The opposite was observed in cattle. Belonging to an M. bovis-infected social group was a significant risk factor for mycobacterial infection in red deer and wild boar, but not for fallow deer. M. bovis TPs were usually found closer to water marshland than MOTT.

Conclusions: The diversity of mycobacteria described herein is indicative of multiple introduction events and a complex multi-host and multi-pathogen epidemiology in DNP. Significant changes in the mycobacterial isolate community may have taken place, even in a short time period (1998 to 2007). Aspects of host social organization should be taken into account in wildlife epidemiology. Wildlife in DNP is frequently exposed to different species of non-tuberculous, environmental mycobacteria, which could interact with the immune response to pathogenic mycobacteria, although the effects are unknown. This research highlights the suitability of molecular typing for surveys at small spatial and temporal scales.

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Mycobacterial isolates (in %) identified in red deer, fallow deer and wild boar from Doñana National Park, Spain. A1 to F1 are Mycobacterium bovis isolates as defined in Figure 1.
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Figure 3: Mycobacterial isolates (in %) identified in red deer, fallow deer and wild boar from Doñana National Park, Spain. A1 to F1 are Mycobacterium bovis isolates as defined in Figure 1.

Mentions: We obtained a total of 154 mycobacterial isolates from DNP wildlife. This included 107 M. bovis isolates belonging to 8 different typing patterns (spoligotyping pattern + VNTR profile, TP), and 47 isolates belonging to four MOTT (Table 1). M. bovis TPs and MOTT species were isolated from wild boar (n = 82 isolates), red deer (n = 33 isolates), and fallow deer (n = 39 isolates) (Figure 3). Wild boar and red deer had 5 M. bovis TPs each, whereas fallow deer presented only 2 TPs. The number of different isolates per host (MOTT and M. bovis TPs combined) was 8 in wild boar, 7 in red deer and 5 in fallow deer (Table 1).


Fine-tuning the space, time, and host distribution of mycobacteria in wildlife.

Gortazar C, Torres MJ, Acevedo P, Aznar J, Negro JJ, de la Fuente J, Vicente J - BMC Microbiol. (2011)

Mycobacterial isolates (in %) identified in red deer, fallow deer and wild boar from Doñana National Park, Spain. A1 to F1 are Mycobacterium bovis isolates as defined in Figure 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3040691&req=5

Figure 3: Mycobacterial isolates (in %) identified in red deer, fallow deer and wild boar from Doñana National Park, Spain. A1 to F1 are Mycobacterium bovis isolates as defined in Figure 1.
Mentions: We obtained a total of 154 mycobacterial isolates from DNP wildlife. This included 107 M. bovis isolates belonging to 8 different typing patterns (spoligotyping pattern + VNTR profile, TP), and 47 isolates belonging to four MOTT (Table 1). M. bovis TPs and MOTT species were isolated from wild boar (n = 82 isolates), red deer (n = 33 isolates), and fallow deer (n = 39 isolates) (Figure 3). Wild boar and red deer had 5 M. bovis TPs each, whereas fallow deer presented only 2 TPs. The number of different isolates per host (MOTT and M. bovis TPs combined) was 8 in wild boar, 7 in red deer and 5 in fallow deer (Table 1).

Bottom Line: While only three TPs were detected in wildlife between 1998 and 2003, up to 8 different ones were found during 2006-2007.M. bovis TPs were usually found closer to water marshland than MOTT.This research highlights the suitability of molecular typing for surveys at small spatial and temporal scales.

View Article: PubMed Central - HTML - PubMed

Affiliation: IREC National Wildlife Research Institute (CSIC-UCLM-JCCM), Ciudad Real, Spain.

ABSTRACT

Background: We describe the diversity of two kinds of mycobacteria isolates, environmental mycobacteria and Mycobacterium bovis collected from wild boar, fallow deer, red deer and cattle in Doñana National Park (DNP, Spain), analyzing their association with temporal, spatial and environmental factors.

Results: High diversity of environmental mycobacteria species and M. bovis typing patterns (TPs) were found. When assessing the factors underlying the presence of the most common types of both environmental mycobacteria and M. bovis TPs in DNP, we evidenced (i) host species differences in the occurrence, (ii) spatial structuration and (iii) differences in the degree of spatial association of specific types between host species. Co-infection of a single host by two M. bovis TPs occurred in all three wild ungulate species. In wild boar and red deer, isolation of one group of mycobacteria occurred more frequently in individuals not infected by the other group. While only three TPs were detected in wildlife between 1998 and 2003, up to 8 different ones were found during 2006-2007. The opposite was observed in cattle. Belonging to an M. bovis-infected social group was a significant risk factor for mycobacterial infection in red deer and wild boar, but not for fallow deer. M. bovis TPs were usually found closer to water marshland than MOTT.

Conclusions: The diversity of mycobacteria described herein is indicative of multiple introduction events and a complex multi-host and multi-pathogen epidemiology in DNP. Significant changes in the mycobacterial isolate community may have taken place, even in a short time period (1998 to 2007). Aspects of host social organization should be taken into account in wildlife epidemiology. Wildlife in DNP is frequently exposed to different species of non-tuberculous, environmental mycobacteria, which could interact with the immune response to pathogenic mycobacteria, although the effects are unknown. This research highlights the suitability of molecular typing for surveys at small spatial and temporal scales.

Show MeSH
Related in: MedlinePlus