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The influence of hyperbaric oxygen treatment on the healing of experimental defects filled with different bone graft substitutes.

Sirin Y, Olgac V, Dogru-Abbasoglu S, Tapul L, Aktas S, Soley S - Int J Med Sci (2011)

Bottom Line: To assess potential effects of hyperbaric oxygen (HBOT) on artificial bone grafts, β - Tricalcium phosphate (β-TCP) and calcium phosphate coated bovine bone (CPCBB) substitutes were applied to standard bone defects in rat tibiae.The healing of defects filled with CPCBB was similar to the controls and it did not respond to HBOT.These findings suggested that the HBOT had beneficial effects on the healing of unfilled bone defects and those filled with β-TCP bone substitute but not with CPCBB, indicating a material-specific influence pattern of HBOT.

View Article: PubMed Central - PubMed

Affiliation: Istanbul University, Faculty of Dentistry, Department of Oral Surgery, Istanbul, Turkey. ysirin@istanbul.edu.tr

ABSTRACT
To assess potential effects of hyperbaric oxygen (HBOT) on artificial bone grafts, β - Tricalcium phosphate (β-TCP) and calcium phosphate coated bovine bone (CPCBB) substitutes were applied to standard bone defects in rat tibiae. The control defects were left empty. Half of the animals received 60 minutes of 2.4 atmosphere absolute (ATA) of HBOT. Rats were sacrificed at one, two and four weeks. Bone healing was assessed histologically and histomorphometrically using light microscopy. The periosteum over the bone defects was examined ultrastructurally. Cardiac blood was collected to determine the serum osteocalcin levels. The HBOT increased new bone formation in the unfilled controls and β-TCP groups and significantly decreased cartilage matrix and fibrous tissue formations in all groups. Active osteoblasts and highly organized collagen fibrils were prominent in the periosteum of β-TCP and control groups. Serum osteocalcin levels also increased with HBOT. The healing of defects filled with CPCBB was similar to the controls and it did not respond to HBOT. These findings suggested that the HBOT had beneficial effects on the healing of unfilled bone defects and those filled with β-TCP bone substitute but not with CPCBB, indicating a material-specific influence pattern of HBOT.

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The histological view of the defect which is nearly filled with newly formed bone in the control groups which had received four week of HBOT (H&E×200) (Nbf; New bone formation, Bm; Bone marrow).
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Figure 4: The histological view of the defect which is nearly filled with newly formed bone in the control groups which had received four week of HBOT (H&E×200) (Nbf; New bone formation, Bm; Bone marrow).

Mentions: In groups which received HBOT, overall bone healing pattern was similar to non-HBO groups. However, from a subjective point of view, new bone formation was occupying larger areas which were previously filled by cartilage and fibrous connective tissues in groups which did not receive HBOT. At one week, there was no prominent inflammatory cell reaction in any of the three groups. Loose connective tissue and new bone trabeculae were found together with small islands of isolated cartilage tissue and abundant new vessels in the control group and around the bone grafts (Figure 1b, 1c). At two weeks, no cartilage or necrotic tissue formation was observed in the control group. Moreover, most of defects were already filled with newly formed bone. Similar observations were made in the groups in which the bone grafts and HBOT were used together. There were also prominent new vessel formations within the connective tissue surrounding the graft particles (Figure 1d). At four weeks, nearly all of the fibrous tissue was replaced by the newly formed bone in the control group. In β - TCP + HBOT group, there was some areas of residual graft particles surrounded mainly by a combination of mature and immature bone. Smaller areas containing graft particles were also present in CPCBB + HBOT group, however, the healing of defects in this group were close to that of the control group (Figure 2, 3 and 4).


The influence of hyperbaric oxygen treatment on the healing of experimental defects filled with different bone graft substitutes.

Sirin Y, Olgac V, Dogru-Abbasoglu S, Tapul L, Aktas S, Soley S - Int J Med Sci (2011)

The histological view of the defect which is nearly filled with newly formed bone in the control groups which had received four week of HBOT (H&E×200) (Nbf; New bone formation, Bm; Bone marrow).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3039227&req=5

Figure 4: The histological view of the defect which is nearly filled with newly formed bone in the control groups which had received four week of HBOT (H&E×200) (Nbf; New bone formation, Bm; Bone marrow).
Mentions: In groups which received HBOT, overall bone healing pattern was similar to non-HBO groups. However, from a subjective point of view, new bone formation was occupying larger areas which were previously filled by cartilage and fibrous connective tissues in groups which did not receive HBOT. At one week, there was no prominent inflammatory cell reaction in any of the three groups. Loose connective tissue and new bone trabeculae were found together with small islands of isolated cartilage tissue and abundant new vessels in the control group and around the bone grafts (Figure 1b, 1c). At two weeks, no cartilage or necrotic tissue formation was observed in the control group. Moreover, most of defects were already filled with newly formed bone. Similar observations were made in the groups in which the bone grafts and HBOT were used together. There were also prominent new vessel formations within the connective tissue surrounding the graft particles (Figure 1d). At four weeks, nearly all of the fibrous tissue was replaced by the newly formed bone in the control group. In β - TCP + HBOT group, there was some areas of residual graft particles surrounded mainly by a combination of mature and immature bone. Smaller areas containing graft particles were also present in CPCBB + HBOT group, however, the healing of defects in this group were close to that of the control group (Figure 2, 3 and 4).

Bottom Line: To assess potential effects of hyperbaric oxygen (HBOT) on artificial bone grafts, β - Tricalcium phosphate (β-TCP) and calcium phosphate coated bovine bone (CPCBB) substitutes were applied to standard bone defects in rat tibiae.The healing of defects filled with CPCBB was similar to the controls and it did not respond to HBOT.These findings suggested that the HBOT had beneficial effects on the healing of unfilled bone defects and those filled with β-TCP bone substitute but not with CPCBB, indicating a material-specific influence pattern of HBOT.

View Article: PubMed Central - PubMed

Affiliation: Istanbul University, Faculty of Dentistry, Department of Oral Surgery, Istanbul, Turkey. ysirin@istanbul.edu.tr

ABSTRACT
To assess potential effects of hyperbaric oxygen (HBOT) on artificial bone grafts, β - Tricalcium phosphate (β-TCP) and calcium phosphate coated bovine bone (CPCBB) substitutes were applied to standard bone defects in rat tibiae. The control defects were left empty. Half of the animals received 60 minutes of 2.4 atmosphere absolute (ATA) of HBOT. Rats were sacrificed at one, two and four weeks. Bone healing was assessed histologically and histomorphometrically using light microscopy. The periosteum over the bone defects was examined ultrastructurally. Cardiac blood was collected to determine the serum osteocalcin levels. The HBOT increased new bone formation in the unfilled controls and β-TCP groups and significantly decreased cartilage matrix and fibrous tissue formations in all groups. Active osteoblasts and highly organized collagen fibrils were prominent in the periosteum of β-TCP and control groups. Serum osteocalcin levels also increased with HBOT. The healing of defects filled with CPCBB was similar to the controls and it did not respond to HBOT. These findings suggested that the HBOT had beneficial effects on the healing of unfilled bone defects and those filled with β-TCP bone substitute but not with CPCBB, indicating a material-specific influence pattern of HBOT.

Show MeSH
Related in: MedlinePlus