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Gene expression changes in a rat model of oxygen-induced retinopathy.

Lee NE, Park YJ, Chung IY, Seo SW, Park JM, Yoo JM, Song JK - Korean J Ophthalmol (2011)

Bottom Line: Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.We found a significant increase in expression of 10 genes (CaM-kinase II inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor; interferon α-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin]; tubulin, β5; fibrillin-1; spectrin β2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.This study should contribute to the understanding of genetic indicators associated with OIR.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju, Korea.

ABSTRACT

Purpose: To identify altered patterns of retinal mRNA expression in a rat model of oxygen-induced retinopathy (OIR).

Methods: Sprague-Dawley rats from P2 to P14 were exposed to hyperoxia (80% oxygen) to induce OIR and then returned to normoxic conditions. Control rats were sustained in room air. Retinal gene expression between the rats of OIR and the controls was compared using cDNA microarray analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.

Results: Among a total of 12,731 cDNAs analyzed by microarray, 13 genes were strongly up- or down-regulated (>2-fold change over controls) in the OIR rats. We found a significant increase in expression of 10 genes (CaM-kinase II inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor; interferon α-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin]; tubulin, β5; fibrillin-1; spectrin β2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.

Conclusions: We confirmed changes in expressions of various retinal genes in a rat model of OIR by microarray and RT-PCR. This study should contribute to the understanding of genetic indicators associated with OIR.

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Related in: MedlinePlus

Down-regulated genes of oxygen-induced retinopathy (OIR) rat retina in reverse transcriptase polymerase chain reaction. CTL = control group; P = postnatal.
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Figure 3: Down-regulated genes of oxygen-induced retinopathy (OIR) rat retina in reverse transcriptase polymerase chain reaction. CTL = control group; P = postnatal.

Mentions: Up-regulated expression pattern (Fig. 2) observed through microarray analysis for gene such as interferon α-inducible protein 27-like, VEGF, hypoxia inducible factor 1a, stromal cell-derived factor 1, intercellular adhesion molecule 1, glucose tolerance factor and annexin II and down-regulated gene expression pattern (Fig. 3) for genes such as heat shock protein and occluding were confirmed by RT-PCR.


Gene expression changes in a rat model of oxygen-induced retinopathy.

Lee NE, Park YJ, Chung IY, Seo SW, Park JM, Yoo JM, Song JK - Korean J Ophthalmol (2011)

Down-regulated genes of oxygen-induced retinopathy (OIR) rat retina in reverse transcriptase polymerase chain reaction. CTL = control group; P = postnatal.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3039194&req=5

Figure 3: Down-regulated genes of oxygen-induced retinopathy (OIR) rat retina in reverse transcriptase polymerase chain reaction. CTL = control group; P = postnatal.
Mentions: Up-regulated expression pattern (Fig. 2) observed through microarray analysis for gene such as interferon α-inducible protein 27-like, VEGF, hypoxia inducible factor 1a, stromal cell-derived factor 1, intercellular adhesion molecule 1, glucose tolerance factor and annexin II and down-regulated gene expression pattern (Fig. 3) for genes such as heat shock protein and occluding were confirmed by RT-PCR.

Bottom Line: Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.We found a significant increase in expression of 10 genes (CaM-kinase II inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor; interferon α-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin]; tubulin, β5; fibrillin-1; spectrin β2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.This study should contribute to the understanding of genetic indicators associated with OIR.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju, Korea.

ABSTRACT

Purpose: To identify altered patterns of retinal mRNA expression in a rat model of oxygen-induced retinopathy (OIR).

Methods: Sprague-Dawley rats from P2 to P14 were exposed to hyperoxia (80% oxygen) to induce OIR and then returned to normoxic conditions. Control rats were sustained in room air. Retinal gene expression between the rats of OIR and the controls was compared using cDNA microarray analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.

Results: Among a total of 12,731 cDNAs analyzed by microarray, 13 genes were strongly up- or down-regulated (>2-fold change over controls) in the OIR rats. We found a significant increase in expression of 10 genes (CaM-kinase II inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor; interferon α-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin]; tubulin, β5; fibrillin-1; spectrin β2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.

Conclusions: We confirmed changes in expressions of various retinal genes in a rat model of OIR by microarray and RT-PCR. This study should contribute to the understanding of genetic indicators associated with OIR.

Show MeSH
Related in: MedlinePlus