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Antimicrobial activities of the methanol extract, fractions and compounds from Ficus polita Vahl. (Moraceae).

Kuete V, Kamga J, Sandjo LP, Ngameni B, Poumale HM, Ambassa P, Ngadjui BT - BMC Complement Altern Med (2011)

Bottom Line: The corresponding value for fractions of 32 μg/ml was obtained on Salmonella typhi, Escherichia coli and Candida albicans ATCC strains.The MIC values recorded with compound 8 on the resistant Pseudomonas aeruginosa PA01 strain was equal to that of chloramphenicol used as reference antibiotic.The obtained results highlighted the interesting antimicrobial potency of F. polita as well as that of compound 8, and provided scientific basis for the traditional use of this taxon in the treatment of microbial infections.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon. kuetevictor@yahoo.fr

ABSTRACT

Background: Many plants of the family Moraceae are used in the treatment of infectious diseases. Ficus polita Vahl., an edible plant belonging to this family is used traditionally in case of dyspepsia, infectious diseases, abdominal pains and diarrhea. The present work was designed to assess the antimicrobial activity of the methanol extract from the roots of F. polita (FPR), as well as that of its fractions (FPR1-5) and two of the eight isolated compounds, namely euphol-3-O-cinnamate (1) and (E)-3,5,4'-trihydroxy-stilbene-3,5-O-β-D-diglucopyranoside (8).

Methods: The liquid microdilution assay was used in the determination of the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC), against seven bacterial and one fungal species.

Results: The results of the MIC determination showed that the crude extract, fractions FPR1, FPR2 and compound 8 were able to prevent the growth of the eight tested microorganisms. Other samples showed selective activity. The lowest MIC value of 64 μg/ml for the crude extract was recorded on 50% of the studied microbial species. The corresponding value for fractions of 32 μg/ml was obtained on Salmonella typhi, Escherichia coli and Candida albicans ATCC strains. The MIC values recorded with compound 8 on the resistant Pseudomonas aeruginosa PA01 strain was equal to that of chloramphenicol used as reference antibiotic.

Conclusion: The obtained results highlighted the interesting antimicrobial potency of F. polita as well as that of compound 8, and provided scientific basis for the traditional use of this taxon in the treatment of microbial infections.

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Related in: MedlinePlus

Chemical structures of compounds isolated from the roots of Ficus polita. 1: euphol-3-O-cinnamate; 2: lupeol; 3: taraxar-14-ene; 4: ursolic acid; 5: ß-sitosterol, 6: betulinic acid; 7: sitosterol-3-O-ß-D-glucopyranoside; 8: (E)-3,5,4'-trihydroxy-stilbene-3,5-O-β-D-diglucopyranoside.
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Figure 1: Chemical structures of compounds isolated from the roots of Ficus polita. 1: euphol-3-O-cinnamate; 2: lupeol; 3: taraxar-14-ene; 4: ursolic acid; 5: ß-sitosterol, 6: betulinic acid; 7: sitosterol-3-O-ß-D-glucopyranoside; 8: (E)-3,5,4'-trihydroxy-stilbene-3,5-O-β-D-diglucopyranoside.

Mentions: The air-dried and powdered root (3.1 kg) was soaked in 10 L of methanol for 48 h, at room temperature. The methanol extract was concentrated under reduced pressure to give 216 g of a brown residue that constituted the crude extract (FPR). Part of FPR (200 g) was submitted to silica gel 60 (0.04-0.063 mm, 200 g) vacuum flash chromatography using as eluent, hexane (Hex), hexane-ethyl acetate (Hex-EtOAc) mixture of increasing polarity, and methanol (MeOH). This was conducted in accordance to the previously reported procedure [14]. Five fractions were obtained, FPR1 (from Hex, 23 g), FPR2 (Hex-EtOAc 75%; 18 g), FPR3 (Hex-EtOAc 50%, 14 g), FPR4 (EtOAc, 12 g) and FPR5 (MeOH, 19 g). A part from FPR3, other fractions, upon antimicrobial assay were subjected to further purification. FPR1 (20 g) was column chromatographed using silica gel 60 (65 g) and Hex-EtOAc gradient as eluent. One hundred and forty seven (147) fractions of 50 ml each were collected. Sub-fractions 2-7 obtained with Hex-EtOAc 2.5% and combined on the basis of TLC analysis afforded compound 1 [18]. Sub-fraction 34-40 (Hex-EtOAc 10%) yielded compound 2 [19]. Sub-fractions 51-57 (Hex-EtOAc 15%) yielded compound 3 [20], while sub-fractions 76-81 obtained in Hex-EtOAc 22.5% gave compound 4 [21]. FPR2 was subjected to column chromatography (CC) similarly to FPR1 and 76 fractions of 50 ml each were collected. Sub-fractions 26-33 eluted with Hex-EtEtOAc 15% yielded compound 5 [22] while sub-fractions 38-41 eluted with Hex-EtEtOAc 20% afforded compound 6 [23]. FPR4 was subjected to CC similarly to FPR1 and 2 using CH2Cl2-MeOH gradient as eluent and 107 sub-fractions were collected. Sub-fractions obtained in CH2Cl2-MeOH 5% afforded compound 7 [22]. FPR5 was also subjected to CC similarly to FPR4 and 93 sub-fractions were collected. Sub-fractions obtained in CH2Cl2-MeOH 10% afforded compound 8 [22]. The chemical structures of the isolated compounds are illustrated in Figure 1.


Antimicrobial activities of the methanol extract, fractions and compounds from Ficus polita Vahl. (Moraceae).

Kuete V, Kamga J, Sandjo LP, Ngameni B, Poumale HM, Ambassa P, Ngadjui BT - BMC Complement Altern Med (2011)

Chemical structures of compounds isolated from the roots of Ficus polita. 1: euphol-3-O-cinnamate; 2: lupeol; 3: taraxar-14-ene; 4: ursolic acid; 5: ß-sitosterol, 6: betulinic acid; 7: sitosterol-3-O-ß-D-glucopyranoside; 8: (E)-3,5,4'-trihydroxy-stilbene-3,5-O-β-D-diglucopyranoside.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037948&req=5

Figure 1: Chemical structures of compounds isolated from the roots of Ficus polita. 1: euphol-3-O-cinnamate; 2: lupeol; 3: taraxar-14-ene; 4: ursolic acid; 5: ß-sitosterol, 6: betulinic acid; 7: sitosterol-3-O-ß-D-glucopyranoside; 8: (E)-3,5,4'-trihydroxy-stilbene-3,5-O-β-D-diglucopyranoside.
Mentions: The air-dried and powdered root (3.1 kg) was soaked in 10 L of methanol for 48 h, at room temperature. The methanol extract was concentrated under reduced pressure to give 216 g of a brown residue that constituted the crude extract (FPR). Part of FPR (200 g) was submitted to silica gel 60 (0.04-0.063 mm, 200 g) vacuum flash chromatography using as eluent, hexane (Hex), hexane-ethyl acetate (Hex-EtOAc) mixture of increasing polarity, and methanol (MeOH). This was conducted in accordance to the previously reported procedure [14]. Five fractions were obtained, FPR1 (from Hex, 23 g), FPR2 (Hex-EtOAc 75%; 18 g), FPR3 (Hex-EtOAc 50%, 14 g), FPR4 (EtOAc, 12 g) and FPR5 (MeOH, 19 g). A part from FPR3, other fractions, upon antimicrobial assay were subjected to further purification. FPR1 (20 g) was column chromatographed using silica gel 60 (65 g) and Hex-EtOAc gradient as eluent. One hundred and forty seven (147) fractions of 50 ml each were collected. Sub-fractions 2-7 obtained with Hex-EtOAc 2.5% and combined on the basis of TLC analysis afforded compound 1 [18]. Sub-fraction 34-40 (Hex-EtOAc 10%) yielded compound 2 [19]. Sub-fractions 51-57 (Hex-EtOAc 15%) yielded compound 3 [20], while sub-fractions 76-81 obtained in Hex-EtOAc 22.5% gave compound 4 [21]. FPR2 was subjected to column chromatography (CC) similarly to FPR1 and 76 fractions of 50 ml each were collected. Sub-fractions 26-33 eluted with Hex-EtEtOAc 15% yielded compound 5 [22] while sub-fractions 38-41 eluted with Hex-EtEtOAc 20% afforded compound 6 [23]. FPR4 was subjected to CC similarly to FPR1 and 2 using CH2Cl2-MeOH gradient as eluent and 107 sub-fractions were collected. Sub-fractions obtained in CH2Cl2-MeOH 5% afforded compound 7 [22]. FPR5 was also subjected to CC similarly to FPR4 and 93 sub-fractions were collected. Sub-fractions obtained in CH2Cl2-MeOH 10% afforded compound 8 [22]. The chemical structures of the isolated compounds are illustrated in Figure 1.

Bottom Line: The corresponding value for fractions of 32 μg/ml was obtained on Salmonella typhi, Escherichia coli and Candida albicans ATCC strains.The MIC values recorded with compound 8 on the resistant Pseudomonas aeruginosa PA01 strain was equal to that of chloramphenicol used as reference antibiotic.The obtained results highlighted the interesting antimicrobial potency of F. polita as well as that of compound 8, and provided scientific basis for the traditional use of this taxon in the treatment of microbial infections.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon. kuetevictor@yahoo.fr

ABSTRACT

Background: Many plants of the family Moraceae are used in the treatment of infectious diseases. Ficus polita Vahl., an edible plant belonging to this family is used traditionally in case of dyspepsia, infectious diseases, abdominal pains and diarrhea. The present work was designed to assess the antimicrobial activity of the methanol extract from the roots of F. polita (FPR), as well as that of its fractions (FPR1-5) and two of the eight isolated compounds, namely euphol-3-O-cinnamate (1) and (E)-3,5,4'-trihydroxy-stilbene-3,5-O-β-D-diglucopyranoside (8).

Methods: The liquid microdilution assay was used in the determination of the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC), against seven bacterial and one fungal species.

Results: The results of the MIC determination showed that the crude extract, fractions FPR1, FPR2 and compound 8 were able to prevent the growth of the eight tested microorganisms. Other samples showed selective activity. The lowest MIC value of 64 μg/ml for the crude extract was recorded on 50% of the studied microbial species. The corresponding value for fractions of 32 μg/ml was obtained on Salmonella typhi, Escherichia coli and Candida albicans ATCC strains. The MIC values recorded with compound 8 on the resistant Pseudomonas aeruginosa PA01 strain was equal to that of chloramphenicol used as reference antibiotic.

Conclusion: The obtained results highlighted the interesting antimicrobial potency of F. polita as well as that of compound 8, and provided scientific basis for the traditional use of this taxon in the treatment of microbial infections.

Show MeSH
Related in: MedlinePlus