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Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.

Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E - Vet. Res. (2011)

Bottom Line: Several types of APC were infected with 39 PRRSV isolates.The results show that different isolates were able to induce different patterns of IL-10 and TNF-α.The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain. mariona.gimeno@cresa.uab.cat.

ABSTRACT
The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-α. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.

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Related in: MedlinePlus

Effect of IL-10 blocking in BDMC infected with two different PRRSV strains upon expression of SLA-I, SLA-II, CD80/86 and CD14. Black solid lines represent the results obtained incubating BMDC with PRRSV in plain culture medium and red dotted lines represent the results obtained after IL-10 blocking.
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Figure 3: Effect of IL-10 blocking in BDMC infected with two different PRRSV strains upon expression of SLA-I, SLA-II, CD80/86 and CD14. Black solid lines represent the results obtained incubating BMDC with PRRSV in plain culture medium and red dotted lines represent the results obtained after IL-10 blocking.

Mentions: As Figure 3 illustrates, blocking of IL-10 resulted in upregulation of the expression of SLA-I, downregulated CD14 and had no effect (strain 2988 IL-10+/TNF-α-) or downregulated (strain 3262 IL-10+/TNF-α+) CD80/86 and SLA-II.


Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.

Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E - Vet. Res. (2011)

Effect of IL-10 blocking in BDMC infected with two different PRRSV strains upon expression of SLA-I, SLA-II, CD80/86 and CD14. Black solid lines represent the results obtained incubating BMDC with PRRSV in plain culture medium and red dotted lines represent the results obtained after IL-10 blocking.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037899&req=5

Figure 3: Effect of IL-10 blocking in BDMC infected with two different PRRSV strains upon expression of SLA-I, SLA-II, CD80/86 and CD14. Black solid lines represent the results obtained incubating BMDC with PRRSV in plain culture medium and red dotted lines represent the results obtained after IL-10 blocking.
Mentions: As Figure 3 illustrates, blocking of IL-10 resulted in upregulation of the expression of SLA-I, downregulated CD14 and had no effect (strain 2988 IL-10+/TNF-α-) or downregulated (strain 3262 IL-10+/TNF-α+) CD80/86 and SLA-II.

Bottom Line: Several types of APC were infected with 39 PRRSV isolates.The results show that different isolates were able to induce different patterns of IL-10 and TNF-α.The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain. mariona.gimeno@cresa.uab.cat.

ABSTRACT
The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-α. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.

Show MeSH
Related in: MedlinePlus