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Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.

Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E - Vet. Res. (2011)

Bottom Line: Several types of APC were infected with 39 PRRSV isolates.The results show that different isolates were able to induce different patterns of IL-10 and TNF-α.The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain. mariona.gimeno@cresa.uab.cat.

ABSTRACT
The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-α. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.

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Dual colour flow cytometry analysis of bone marrow-derived dendritic cells at 48 h post inoculation with different PRRSV isolates at 0.01 m.o.i.: A) SLA-II and CD80/86; B) SLA-II and CD163.
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Figure 2: Dual colour flow cytometry analysis of bone marrow-derived dendritic cells at 48 h post inoculation with different PRRSV isolates at 0.01 m.o.i.: A) SLA-II and CD80/86; B) SLA-II and CD163.

Mentions: Examination of BMDC 48 h after inoculation with the virus showed that strains with different cytokine-inducing properties regulated several cell markers differently (Figure 1 and Table 3). For SLA I, compared to mock-inoculated cells, strains 3267, 3249 and 3262 produced a decrease in the expression of that molecule while no changes were observed for strain 2988. For SLA-II, all strains but 3249 produced significant increases in expression (p < 0.03) compared to uninfected cells. For CD80/86, the behaviour of BMDC after infection differed depending on the strains. Interestingly, the double negative cytokine-inducing strain (3267) was the one that produced the highest increase in the expression of CD80/86 while the double positive strain (3262) produced a decrease in CD80/86 compared with the uninfected control (p = 0.01). For CD14, compared to the uninfected cells, the expression increased for strain 3262 (double positive cytokine-inducing strain), decreased for strain 3267 (double negative cytokine-inducing strain) and no changes were observed for strain 3249 and 2988. Regarding CD163, expression decreased only in the double negative strain (3267). When strain 3249 was used for stimulation, CD163 showed a trend for an increase although the p-value was not strictly significant (p = 0.09). Compared to uninfected cells, CD16 expression was always reduced upon PRRSV inoculation (p = 0.02) except for isolate 2988 and no differences were found for SwC9 and SWC3 expression. To further gain insight into these changes, SLA-II, CD80/86, and CD163 were examined in double staining experiments (Figure 2). The results show that strain 3262 (IL-10+/TNF-α+) promoted an increase in the expression of single SLA-II+ (76% in inoculated cells versus 63% in mock-inoculated cells) simultaneously with a reduction of CD80/86 expression. It is worthy to note the decrease in the double positive subset percentage (7% in mock-infected cells; 3% in 3262-inoculated cells). In contrast, cells infected with 3267 (IL-10-/TNF-α-) exhibited an increase (75.7%) in the total proportion of SLA-II+ single expressing cells but the numbers of SLA-II-/CD80/86+ cells increased (from 8.5% in uninfected cells to 13.0% in 3267 infected cultures). For CD163, IL-10 inducing strains were able to keep the proportions of double positive SLAII/CD163 cells compared to mock-inoculated culture cells while infection with a IL-10-/TNF- strain (3267) produced a clear decline of double positive SLAII/CD163 cells.


Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.

Gimeno M, Darwich L, Diaz I, de la Torre E, Pujols J, Martín M, Inumaru S, Cano E, Domingo M, Montoya M, Mateu E - Vet. Res. (2011)

Dual colour flow cytometry analysis of bone marrow-derived dendritic cells at 48 h post inoculation with different PRRSV isolates at 0.01 m.o.i.: A) SLA-II and CD80/86; B) SLA-II and CD163.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037899&req=5

Figure 2: Dual colour flow cytometry analysis of bone marrow-derived dendritic cells at 48 h post inoculation with different PRRSV isolates at 0.01 m.o.i.: A) SLA-II and CD80/86; B) SLA-II and CD163.
Mentions: Examination of BMDC 48 h after inoculation with the virus showed that strains with different cytokine-inducing properties regulated several cell markers differently (Figure 1 and Table 3). For SLA I, compared to mock-inoculated cells, strains 3267, 3249 and 3262 produced a decrease in the expression of that molecule while no changes were observed for strain 2988. For SLA-II, all strains but 3249 produced significant increases in expression (p < 0.03) compared to uninfected cells. For CD80/86, the behaviour of BMDC after infection differed depending on the strains. Interestingly, the double negative cytokine-inducing strain (3267) was the one that produced the highest increase in the expression of CD80/86 while the double positive strain (3262) produced a decrease in CD80/86 compared with the uninfected control (p = 0.01). For CD14, compared to the uninfected cells, the expression increased for strain 3262 (double positive cytokine-inducing strain), decreased for strain 3267 (double negative cytokine-inducing strain) and no changes were observed for strain 3249 and 2988. Regarding CD163, expression decreased only in the double negative strain (3267). When strain 3249 was used for stimulation, CD163 showed a trend for an increase although the p-value was not strictly significant (p = 0.09). Compared to uninfected cells, CD16 expression was always reduced upon PRRSV inoculation (p = 0.02) except for isolate 2988 and no differences were found for SwC9 and SWC3 expression. To further gain insight into these changes, SLA-II, CD80/86, and CD163 were examined in double staining experiments (Figure 2). The results show that strain 3262 (IL-10+/TNF-α+) promoted an increase in the expression of single SLA-II+ (76% in inoculated cells versus 63% in mock-inoculated cells) simultaneously with a reduction of CD80/86 expression. It is worthy to note the decrease in the double positive subset percentage (7% in mock-infected cells; 3% in 3262-inoculated cells). In contrast, cells infected with 3267 (IL-10-/TNF-α-) exhibited an increase (75.7%) in the total proportion of SLA-II+ single expressing cells but the numbers of SLA-II-/CD80/86+ cells increased (from 8.5% in uninfected cells to 13.0% in 3267 infected cultures). For CD163, IL-10 inducing strains were able to keep the proportions of double positive SLAII/CD163 cells compared to mock-inoculated culture cells while infection with a IL-10-/TNF- strain (3267) produced a clear decline of double positive SLAII/CD163 cells.

Bottom Line: Several types of APC were infected with 39 PRRSV isolates.The results show that different isolates were able to induce different patterns of IL-10 and TNF-α.The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain. mariona.gimeno@cresa.uab.cat.

ABSTRACT
The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-α. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.

Show MeSH
Related in: MedlinePlus