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Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli.

Pezeshki A, Stordeur P, Wallemacq H, Schynts F, Stevens M, Boutet P, Peelman LJ, De Spiegeleer B, Duchateau L, Bureau F, Burvenich C - Vet. Res. (2011)

Bottom Line: The infrared images were taken from the caudal view of the udder following challenge with E. coli.Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-α (TNF-α) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity.Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group, Ghent University, B-9000 Ghent, Belgium. Christian.Burvenich@UGent.be.

ABSTRACT
The objective of the current study was to investigate (i) the outcome of experimentally induced Escherichia coli mastitis in primiparous cows during early lactation in relation with production of eicosanoids and inflammatory indicators, and (ii) the validity of thermography to evaluate temperature changes on udder skin surface after experimentally induced E. coli mastitis. Nine primiparous Holstein Friesian cows were inoculated 24 ± 6 days (d) after parturition in both left quarters with E. coli P4 serotype O32:H37. Blood and milk samples were collected before and after challenge with E. coli. The infrared images were taken from the caudal view of the udder following challenge with E. coli. No relationship was detected between severity of mastitis and changes of thromboxane B2 (TXB2), leukotriene B4 (LTB4) and lipoxin A4 (LXA4). However, prostaglandin E2 (PGE2) was related to systemic disease severity during E. coli mastitis. Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-α (TNF-α) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity. The thermal camera was capable of detecting 2-3 °C temperature changes on udder skin surface of cows inoculated with E. coli. Peak of udder skin temperature occurred after peak of rectal temperature and appearance of local signs of induced E. coli mastitis. Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.

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Prostaglandin E2 (A), thromboxane B2 (B), leukotriene B4 (C) and lipoxin A4 (D) in secreta of all infused quarters at various times following intramammary infection. See legend of Figure 2 for more details.
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Figure 4: Prostaglandin E2 (A), thromboxane B2 (B), leukotriene B4 (C) and lipoxin A4 (D) in secreta of all infused quarters at various times following intramammary infection. See legend of Figure 2 for more details.

Mentions: In milk, the mean PGE2 concentration immediately after intramammary inoculation was 2572.9 ± 1155.6 pg/mL. Overall, there was a significant time effect for PGE2 (P < 0.01; Figure 4A). There was a relationship between disease severity and PGE2 concentration (P < 0.0001), with higher PGE2 concentrations corresponding to higher disease severity. The mean concentration of milk TXB2 immediately after infusion of E. coli was 1504.3 ± 1267.7 pg/mL. After infusion of E. coli, TXB2 changed significantly over time (P < 0.0001; Figure 4B). TXB2 started to increase from PIH 9 onward and returned to its normal level after PIH 24. Immediately after infusion of E. coli, the mean concentration of LTB4 was 2270.7 ± 776.5 pg/mL. There was no significant effect of time for concentration of LTB4 in milk (Figure 4C). In milk, the mean concentration of LXA4 immediately after infusion was 3513.9 ± 2108.1 pg/mL. The effect of time on the concentration of LXA4 was significant (P < 0.0001; Figure 4D). LXA4 decreased gradually after intramammary infusion of E. coli and did not normalize before 144 PIH. We failed to detect any relationship between disease severity and concentrations of TXB2, LTB4 and LXA4.


Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli.

Pezeshki A, Stordeur P, Wallemacq H, Schynts F, Stevens M, Boutet P, Peelman LJ, De Spiegeleer B, Duchateau L, Bureau F, Burvenich C - Vet. Res. (2011)

Prostaglandin E2 (A), thromboxane B2 (B), leukotriene B4 (C) and lipoxin A4 (D) in secreta of all infused quarters at various times following intramammary infection. See legend of Figure 2 for more details.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037895&req=5

Figure 4: Prostaglandin E2 (A), thromboxane B2 (B), leukotriene B4 (C) and lipoxin A4 (D) in secreta of all infused quarters at various times following intramammary infection. See legend of Figure 2 for more details.
Mentions: In milk, the mean PGE2 concentration immediately after intramammary inoculation was 2572.9 ± 1155.6 pg/mL. Overall, there was a significant time effect for PGE2 (P < 0.01; Figure 4A). There was a relationship between disease severity and PGE2 concentration (P < 0.0001), with higher PGE2 concentrations corresponding to higher disease severity. The mean concentration of milk TXB2 immediately after infusion of E. coli was 1504.3 ± 1267.7 pg/mL. After infusion of E. coli, TXB2 changed significantly over time (P < 0.0001; Figure 4B). TXB2 started to increase from PIH 9 onward and returned to its normal level after PIH 24. Immediately after infusion of E. coli, the mean concentration of LTB4 was 2270.7 ± 776.5 pg/mL. There was no significant effect of time for concentration of LTB4 in milk (Figure 4C). In milk, the mean concentration of LXA4 immediately after infusion was 3513.9 ± 2108.1 pg/mL. The effect of time on the concentration of LXA4 was significant (P < 0.0001; Figure 4D). LXA4 decreased gradually after intramammary infusion of E. coli and did not normalize before 144 PIH. We failed to detect any relationship between disease severity and concentrations of TXB2, LTB4 and LXA4.

Bottom Line: The infrared images were taken from the caudal view of the udder following challenge with E. coli.Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-α (TNF-α) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity.Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group, Ghent University, B-9000 Ghent, Belgium. Christian.Burvenich@UGent.be.

ABSTRACT
The objective of the current study was to investigate (i) the outcome of experimentally induced Escherichia coli mastitis in primiparous cows during early lactation in relation with production of eicosanoids and inflammatory indicators, and (ii) the validity of thermography to evaluate temperature changes on udder skin surface after experimentally induced E. coli mastitis. Nine primiparous Holstein Friesian cows were inoculated 24 ± 6 days (d) after parturition in both left quarters with E. coli P4 serotype O32:H37. Blood and milk samples were collected before and after challenge with E. coli. The infrared images were taken from the caudal view of the udder following challenge with E. coli. No relationship was detected between severity of mastitis and changes of thromboxane B2 (TXB2), leukotriene B4 (LTB4) and lipoxin A4 (LXA4). However, prostaglandin E2 (PGE2) was related to systemic disease severity during E. coli mastitis. Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-α (TNF-α) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity. The thermal camera was capable of detecting 2-3 °C temperature changes on udder skin surface of cows inoculated with E. coli. Peak of udder skin temperature occurred after peak of rectal temperature and appearance of local signs of induced E. coli mastitis. Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.

Show MeSH
Related in: MedlinePlus