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Comprehensive expression analysis of prostanoid enzymes and receptors in the human endometrium across the menstrual cycle.

Catalano RD, Wilson MR, Boddy SC, Jabbour HN - Mol. Hum. Reprod. (2010)

Bottom Line: Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system.The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle.This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.

ABSTRACT
Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system. The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle. The analysis identified PTGFR to have a distinct expression profile compared with other components of the prostanoid system, as expression is maximal during the proliferative phase. Immunohistochemical analysis for PTGER1 suggests a dual function for this receptor depending on its temporal (proliferative versus secretory) and spatial (nuclear versus cell membrane) expression. The expression profiles of the PGF(2α) synthases identified AKR1B1 and CBR1 as the likely regulators of PGF(2α) production during the menstrual phase. Immunohistochemical analysis for AKR1B1, CBR1 and AKR1C3 suggest expression to be in the glandular epithelium and vasculature. This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium. The expression profiles described have the potential to identify specific prostanoid components that may be dysregulated in inflammatory-associated disorders of the endometrium.

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Expression of prostanoid biosynthetic enzyme and receptor genes in the menstrual cycle. Summary diagram illustrating the maximal expression during the menstrual cycle for each prostanoid biosynthesis and receptor gene analysed and reported maximum production during the menstrual cycle for each prostanoid (shown in red). WOI, window of implantation. For abbreviations see Table I.
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GAQ094F5: Expression of prostanoid biosynthetic enzyme and receptor genes in the menstrual cycle. Summary diagram illustrating the maximal expression during the menstrual cycle for each prostanoid biosynthesis and receptor gene analysed and reported maximum production during the menstrual cycle for each prostanoid (shown in red). WOI, window of implantation. For abbreviations see Table I.

Mentions: Inflammatory events have been described in the human endometrium during the period of endometrial receptivity and menstruation. These events are characterized by an increase in endometrial capillary dilation, permeability, stromal edema, blood flow, leucocyte number and secretion of pro-inflammatory factors (Fraser et al., 1987; Johannisson et al., 1987; Peek et al., 1992). Pro-inflammatory enzymes PTGS1 and PTGS2 are key mediators of these inflammatory processes. However, the downstream prostanoids and receptors that contribute to these inflammatory events remain unclear. The aim of this study was to provide expression profiles across the menstrual cycle of the genes which constitute the prostanoid biosynthetic and signalling pathways. The data generated in this study are summarized in Fig. 5 and indicate the stage of the menstrual cycle where maximum transcript expression occurs for each gene. The data suggest that distinct components of the prostanoid system are associated with specific stages of the menstrual cycle and expression predominantly increases from the mid-secretory phase through to, and including, menstruation.Figure 5


Comprehensive expression analysis of prostanoid enzymes and receptors in the human endometrium across the menstrual cycle.

Catalano RD, Wilson MR, Boddy SC, Jabbour HN - Mol. Hum. Reprod. (2010)

Expression of prostanoid biosynthetic enzyme and receptor genes in the menstrual cycle. Summary diagram illustrating the maximal expression during the menstrual cycle for each prostanoid biosynthesis and receptor gene analysed and reported maximum production during the menstrual cycle for each prostanoid (shown in red). WOI, window of implantation. For abbreviations see Table I.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3037736&req=5

GAQ094F5: Expression of prostanoid biosynthetic enzyme and receptor genes in the menstrual cycle. Summary diagram illustrating the maximal expression during the menstrual cycle for each prostanoid biosynthesis and receptor gene analysed and reported maximum production during the menstrual cycle for each prostanoid (shown in red). WOI, window of implantation. For abbreviations see Table I.
Mentions: Inflammatory events have been described in the human endometrium during the period of endometrial receptivity and menstruation. These events are characterized by an increase in endometrial capillary dilation, permeability, stromal edema, blood flow, leucocyte number and secretion of pro-inflammatory factors (Fraser et al., 1987; Johannisson et al., 1987; Peek et al., 1992). Pro-inflammatory enzymes PTGS1 and PTGS2 are key mediators of these inflammatory processes. However, the downstream prostanoids and receptors that contribute to these inflammatory events remain unclear. The aim of this study was to provide expression profiles across the menstrual cycle of the genes which constitute the prostanoid biosynthetic and signalling pathways. The data generated in this study are summarized in Fig. 5 and indicate the stage of the menstrual cycle where maximum transcript expression occurs for each gene. The data suggest that distinct components of the prostanoid system are associated with specific stages of the menstrual cycle and expression predominantly increases from the mid-secretory phase through to, and including, menstruation.Figure 5

Bottom Line: Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system.The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle.This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.

ABSTRACT
Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system. The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle. The analysis identified PTGFR to have a distinct expression profile compared with other components of the prostanoid system, as expression is maximal during the proliferative phase. Immunohistochemical analysis for PTGER1 suggests a dual function for this receptor depending on its temporal (proliferative versus secretory) and spatial (nuclear versus cell membrane) expression. The expression profiles of the PGF(2α) synthases identified AKR1B1 and CBR1 as the likely regulators of PGF(2α) production during the menstrual phase. Immunohistochemical analysis for AKR1B1, CBR1 and AKR1C3 suggest expression to be in the glandular epithelium and vasculature. This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium. The expression profiles described have the potential to identify specific prostanoid components that may be dysregulated in inflammatory-associated disorders of the endometrium.

Show MeSH