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Comprehensive expression analysis of prostanoid enzymes and receptors in the human endometrium across the menstrual cycle.

Catalano RD, Wilson MR, Boddy SC, Jabbour HN - Mol. Hum. Reprod. (2010)

Bottom Line: Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system.The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle.This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.

ABSTRACT
Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system. The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle. The analysis identified PTGFR to have a distinct expression profile compared with other components of the prostanoid system, as expression is maximal during the proliferative phase. Immunohistochemical analysis for PTGER1 suggests a dual function for this receptor depending on its temporal (proliferative versus secretory) and spatial (nuclear versus cell membrane) expression. The expression profiles of the PGF(2α) synthases identified AKR1B1 and CBR1 as the likely regulators of PGF(2α) production during the menstrual phase. Immunohistochemical analysis for AKR1B1, CBR1 and AKR1C3 suggest expression to be in the glandular epithelium and vasculature. This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium. The expression profiles described have the potential to identify specific prostanoid components that may be dysregulated in inflammatory-associated disorders of the endometrium.

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Immunohistochemical localization of AKR1C3 (A–E), CBR1 (F–J), AKR1B1 (K–O), PTGDR (P–T) and PTGER1 (U–Y) in the endometrium across the menstrual cycle; proliferative endometrium (A, F, K, P, U), early-secretory endometrium (B, G, L, Q, V), mid-secretory endometrium (C, H, M, R, W), late secretory endometrium (D, I, N, S, X) and menstrual endometrium (E, J, O, T, Y). Negative controls (−ve). For abbreviations see Table I.
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GAQ094F4: Immunohistochemical localization of AKR1C3 (A–E), CBR1 (F–J), AKR1B1 (K–O), PTGDR (P–T) and PTGER1 (U–Y) in the endometrium across the menstrual cycle; proliferative endometrium (A, F, K, P, U), early-secretory endometrium (B, G, L, Q, V), mid-secretory endometrium (C, H, M, R, W), late secretory endometrium (D, I, N, S, X) and menstrual endometrium (E, J, O, T, Y). Negative controls (−ve). For abbreviations see Table I.

Mentions: Immunohistochemical analysis was performed for selected genes which have not been studied previously in the human endometrium across the menstrual cycle. These included AKR1C3, CBR1, AKR1B1, PTGDR and PTGER1. The analysis aimed to determine whether the RNA transcripts detected by Q-RT–PCR were translated into protein products and to determine their spatial and temporal expression across the menstrual cycle. All transcripts detected by RT–PCR were shown to be translated into protein products (Fig. 4). The staining intensity of the immunohistochemistry suggested protein expression was comparable to RNA expression obtained by Q-RT–PCR except for staining of AKR1C3 and AKR1B1 in the mid-secretory phase and PTGER1 in the proliferative phase. These differences suggested a modest decrease in protein expression compared with the RNA expression profile, although caution must be exercised when interpreting immunohistochemistry. Quantitatively interpreting immunostaining between a section with intense localized staining and a section with moderate staining intensity throughout may give a misleading impression of differences in global expression levels.Figure 4


Comprehensive expression analysis of prostanoid enzymes and receptors in the human endometrium across the menstrual cycle.

Catalano RD, Wilson MR, Boddy SC, Jabbour HN - Mol. Hum. Reprod. (2010)

Immunohistochemical localization of AKR1C3 (A–E), CBR1 (F–J), AKR1B1 (K–O), PTGDR (P–T) and PTGER1 (U–Y) in the endometrium across the menstrual cycle; proliferative endometrium (A, F, K, P, U), early-secretory endometrium (B, G, L, Q, V), mid-secretory endometrium (C, H, M, R, W), late secretory endometrium (D, I, N, S, X) and menstrual endometrium (E, J, O, T, Y). Negative controls (−ve). For abbreviations see Table I.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3037736&req=5

GAQ094F4: Immunohistochemical localization of AKR1C3 (A–E), CBR1 (F–J), AKR1B1 (K–O), PTGDR (P–T) and PTGER1 (U–Y) in the endometrium across the menstrual cycle; proliferative endometrium (A, F, K, P, U), early-secretory endometrium (B, G, L, Q, V), mid-secretory endometrium (C, H, M, R, W), late secretory endometrium (D, I, N, S, X) and menstrual endometrium (E, J, O, T, Y). Negative controls (−ve). For abbreviations see Table I.
Mentions: Immunohistochemical analysis was performed for selected genes which have not been studied previously in the human endometrium across the menstrual cycle. These included AKR1C3, CBR1, AKR1B1, PTGDR and PTGER1. The analysis aimed to determine whether the RNA transcripts detected by Q-RT–PCR were translated into protein products and to determine their spatial and temporal expression across the menstrual cycle. All transcripts detected by RT–PCR were shown to be translated into protein products (Fig. 4). The staining intensity of the immunohistochemistry suggested protein expression was comparable to RNA expression obtained by Q-RT–PCR except for staining of AKR1C3 and AKR1B1 in the mid-secretory phase and PTGER1 in the proliferative phase. These differences suggested a modest decrease in protein expression compared with the RNA expression profile, although caution must be exercised when interpreting immunohistochemistry. Quantitatively interpreting immunostaining between a section with intense localized staining and a section with moderate staining intensity throughout may give a misleading impression of differences in global expression levels.Figure 4

Bottom Line: Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system.The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle.This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK.

ABSTRACT
Prostanoids are well-described primary mediators of inflammatory processes and are essential for the normal physiological function of the female reproductive system. The aim of this study was to determine the temporal expression of the prostanoid biosynthetic enzymes (PTGS1, PTGS2, PTGES, PTGES2, PTGES3, AKR1B1, AKR1C3, CBR1, HPGDS, PTGDS, PTGIS, TBXAS1 and HPGD) and the prostanoid receptors (PTGER1, PTGER2, PTGER3, PTGER4, PTGFR, PTGDR, GPR44, PTGIR and TBXA2R) in the human endometrium throughout the menstrual cycle. The analysis identified PTGFR to have a distinct expression profile compared with other components of the prostanoid system, as expression is maximal during the proliferative phase. Immunohistochemical analysis for PTGER1 suggests a dual function for this receptor depending on its temporal (proliferative versus secretory) and spatial (nuclear versus cell membrane) expression. The expression profiles of the PGF(2α) synthases identified AKR1B1 and CBR1 as the likely regulators of PGF(2α) production during the menstrual phase. Immunohistochemical analysis for AKR1B1, CBR1 and AKR1C3 suggest expression to be in the glandular epithelium and vasculature. This study represents the first comprehensive analysis of the components of prostanoid biosynthetic and signalling pathway in the human endometrium. The expression profiles described have the potential to identify specific prostanoid components that may be dysregulated in inflammatory-associated disorders of the endometrium.

Show MeSH