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Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.

Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, Kikuta S, Watanabe M, Cornette R, Okuda T, Kikawada T - FEBS J. (2010)

Bottom Line: Although computational prediction indicated that the alternative splicing variants (PvTpsα/β) obtained encoded probable functional motifs consisting of a typical consensus domain of TPS and a conserved sequence of TPP, PvTpsα did not exert activity as TPP, but only as TPS.The translated product of the TREH ortholog (PvTreh) certainly degraded trehalose to glucose.Trehalose was synthesized abundantly, consistent with increased activities of TPS and TPP and suppressed TREH activity.

View Article: PubMed Central - PubMed

Affiliation: Anhydrobiosis Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

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Expression profiles of mRNAs and proteins of the genes involved in trehalose metabolism during desiccation. Total RNA and protein were prepared from larvae treated under desiccation conditions, and analyzed by northern blotting (A) and western blotting (B).
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fig06: Expression profiles of mRNAs and proteins of the genes involved in trehalose metabolism during desiccation. Total RNA and protein were prepared from larvae treated under desiccation conditions, and analyzed by northern blotting (A) and western blotting (B).

Mentions: As shown in Fig. 1B, in P. vanderplanki, trehalose is likely to be synthesized from glycogen en route to anhydrobiosis. In eukaryotes, the metabolic pathway from glycogen to trehalose is highly conserved (Fig. 1A). Hence, in order to elucidate the molecular mechanisms underlying the regulation of the enzymes involved in trehalose metabolism on desiccation, we first investigated the expression profiles of PvTpsα/β, PvTpp and PvTreh mRNAs (Fig. 6A). The accumulation of PvTpsα/β and PvTpp mRNAs was induced within 1 h and 3 h, respectively, during desiccation treatment. For PvTreh, the induction of mRNA accumulation was delayed by 48 h after the beginning of desiccation treatment compared with the other two genes. Real-time PCR analyses of these mRNAs confirmed the results (data not shown). However, the amount of PvGp mRNAs remained constant during treatment, which is consistent with the constancy of GP activity on desiccation (Fig. 2A). Western blot analyses revealed that the proteins of PvTPSα/β, PvTPP and PvTREH were also accumulated, as were the corresponding mRNAs (Fig. 6B).


Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.

Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, Kikuta S, Watanabe M, Cornette R, Okuda T, Kikawada T - FEBS J. (2010)

Expression profiles of mRNAs and proteins of the genes involved in trehalose metabolism during desiccation. Total RNA and protein were prepared from larvae treated under desiccation conditions, and analyzed by northern blotting (A) and western blotting (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037560&req=5

fig06: Expression profiles of mRNAs and proteins of the genes involved in trehalose metabolism during desiccation. Total RNA and protein were prepared from larvae treated under desiccation conditions, and analyzed by northern blotting (A) and western blotting (B).
Mentions: As shown in Fig. 1B, in P. vanderplanki, trehalose is likely to be synthesized from glycogen en route to anhydrobiosis. In eukaryotes, the metabolic pathway from glycogen to trehalose is highly conserved (Fig. 1A). Hence, in order to elucidate the molecular mechanisms underlying the regulation of the enzymes involved in trehalose metabolism on desiccation, we first investigated the expression profiles of PvTpsα/β, PvTpp and PvTreh mRNAs (Fig. 6A). The accumulation of PvTpsα/β and PvTpp mRNAs was induced within 1 h and 3 h, respectively, during desiccation treatment. For PvTreh, the induction of mRNA accumulation was delayed by 48 h after the beginning of desiccation treatment compared with the other two genes. Real-time PCR analyses of these mRNAs confirmed the results (data not shown). However, the amount of PvGp mRNAs remained constant during treatment, which is consistent with the constancy of GP activity on desiccation (Fig. 2A). Western blot analyses revealed that the proteins of PvTPSα/β, PvTPP and PvTREH were also accumulated, as were the corresponding mRNAs (Fig. 6B).

Bottom Line: Although computational prediction indicated that the alternative splicing variants (PvTpsα/β) obtained encoded probable functional motifs consisting of a typical consensus domain of TPS and a conserved sequence of TPP, PvTpsα did not exert activity as TPP, but only as TPS.The translated product of the TREH ortholog (PvTreh) certainly degraded trehalose to glucose.Trehalose was synthesized abundantly, consistent with increased activities of TPS and TPP and suppressed TREH activity.

View Article: PubMed Central - PubMed

Affiliation: Anhydrobiosis Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

Show MeSH
Related in: MedlinePlus