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Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.

Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, Kikuta S, Watanabe M, Cornette R, Okuda T, Kikawada T - FEBS J. (2010)

Bottom Line: Although computational prediction indicated that the alternative splicing variants (PvTpsα/β) obtained encoded probable functional motifs consisting of a typical consensus domain of TPS and a conserved sequence of TPP, PvTpsα did not exert activity as TPP, but only as TPS.The translated product of the TREH ortholog (PvTreh) certainly degraded trehalose to glucose.Trehalose was synthesized abundantly, consistent with increased activities of TPS and TPP and suppressed TREH activity.

View Article: PubMed Central - PubMed

Affiliation: Anhydrobiosis Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

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Related in: MedlinePlus

Schematic representation of the trehalose metabolic pathway (A) and changes in glycogen and trehalose content in P. vanderplanki larvae during desiccation treatment (B). Filled circles and open circles represent glycogen and trehalose content, respectively; the broken line represents the amount of total carbohydrate. G-1-P, glucose-1-phosphate; G-6-P, glucose-6-phosphate; Glc, glucose; PGM, phosphoglucomutase; UDPGP, UDP-glucose pyrophosphorylase; Pi, inorganic phosphate; PPi, pyrophosphate; T-6-P, trehalose-6-phosphate.
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fig01: Schematic representation of the trehalose metabolic pathway (A) and changes in glycogen and trehalose content in P. vanderplanki larvae during desiccation treatment (B). Filled circles and open circles represent glycogen and trehalose content, respectively; the broken line represents the amount of total carbohydrate. G-1-P, glucose-1-phosphate; G-6-P, glucose-6-phosphate; Glc, glucose; PGM, phosphoglucomutase; UDPGP, UDP-glucose pyrophosphorylase; Pi, inorganic phosphate; PPi, pyrophosphate; T-6-P, trehalose-6-phosphate.

Mentions: In addition to its role as an anhydroprotectant, trehalose is generally known as a carbon and energy source for bacteria and yeast [10]. In bacteria and yeast, trehalose is synthesized from glucose-6-phosphate and UDP-glucose, catalyzed by trehalose-6-phosphate synthase (TPS; EC 2.4.1.15) and trehalose-6-phosphate phosphatase (TPP; EC 3.1.3.12), and the relevant genes have been cloned and well characterized (Fig. 1A). This synthetic pathway is considered to be conserved in a wide range of taxa, including unicellular and multicellular organisms, because these genes have been found in algae, fungi, plants and invertebrates [11].


Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.

Mitsumasu K, Kanamori Y, Fujita M, Iwata K, Tanaka D, Kikuta S, Watanabe M, Cornette R, Okuda T, Kikawada T - FEBS J. (2010)

Schematic representation of the trehalose metabolic pathway (A) and changes in glycogen and trehalose content in P. vanderplanki larvae during desiccation treatment (B). Filled circles and open circles represent glycogen and trehalose content, respectively; the broken line represents the amount of total carbohydrate. G-1-P, glucose-1-phosphate; G-6-P, glucose-6-phosphate; Glc, glucose; PGM, phosphoglucomutase; UDPGP, UDP-glucose pyrophosphorylase; Pi, inorganic phosphate; PPi, pyrophosphate; T-6-P, trehalose-6-phosphate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037560&req=5

fig01: Schematic representation of the trehalose metabolic pathway (A) and changes in glycogen and trehalose content in P. vanderplanki larvae during desiccation treatment (B). Filled circles and open circles represent glycogen and trehalose content, respectively; the broken line represents the amount of total carbohydrate. G-1-P, glucose-1-phosphate; G-6-P, glucose-6-phosphate; Glc, glucose; PGM, phosphoglucomutase; UDPGP, UDP-glucose pyrophosphorylase; Pi, inorganic phosphate; PPi, pyrophosphate; T-6-P, trehalose-6-phosphate.
Mentions: In addition to its role as an anhydroprotectant, trehalose is generally known as a carbon and energy source for bacteria and yeast [10]. In bacteria and yeast, trehalose is synthesized from glucose-6-phosphate and UDP-glucose, catalyzed by trehalose-6-phosphate synthase (TPS; EC 2.4.1.15) and trehalose-6-phosphate phosphatase (TPP; EC 3.1.3.12), and the relevant genes have been cloned and well characterized (Fig. 1A). This synthetic pathway is considered to be conserved in a wide range of taxa, including unicellular and multicellular organisms, because these genes have been found in algae, fungi, plants and invertebrates [11].

Bottom Line: Although computational prediction indicated that the alternative splicing variants (PvTpsα/β) obtained encoded probable functional motifs consisting of a typical consensus domain of TPS and a conserved sequence of TPP, PvTpsα did not exert activity as TPP, but only as TPS.The translated product of the TREH ortholog (PvTreh) certainly degraded trehalose to glucose.Trehalose was synthesized abundantly, consistent with increased activities of TPS and TPP and suppressed TREH activity.

View Article: PubMed Central - PubMed

Affiliation: Anhydrobiosis Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

Show MeSH
Related in: MedlinePlus