Genomic analysis of oceanic cyanobacterial myoviruses compared with T4-like myoviruses from diverse hosts and environments.
Bottom Line: Patterns among non-core genes that may drive niche diversification revealed that phosphorus-related gene content reflects source waters rather than host strain used for isolation, and that carbon metabolism genes appear associated with putative mobile elements.However, no clear diagnostic genes emerged to distinguish these phage groups, suggesting blurred boundaries possibly due to cross-infection.Finally, genome-wide comparisons of both diverse and closely related, co-isolated genomes provide a locus-to-locus variability metric that will prove valuable for interpreting metagenomic data sets.
Affiliation: Massachusetts Institute of Technology, Cambridge, MA, USA. firstname.lastname@example.orgShow MeSH
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Mentions: Three features of the cyanophage genomes suggest that they modulate 2OG levels to stimulate NtcA activity as needed to promote phage gene expression (Fig. 6). First, all 16 genomes contain numerous NtcA binding sites (1–16 per genome; average = 8.9), which apparently promote a diversity of both T4 phage and cyanophage functions (Fig. 1). Second, 14 of the 16 genomes contain numerous 2OG-FeII oxygenase superfamily proteins (Table 3). Third, all 16 cyanophages contain at least one and often numerous hypothetical proteins with possible phytanoyl-CoA-dioxygenase domains, (Table S4), which may act on 2OG, in this case as oxidoreductases.
Affiliation: Massachusetts Institute of Technology, Cambridge, MA, USA. email@example.com