Genomic analysis of oceanic cyanobacterial myoviruses compared with T4-like myoviruses from diverse hosts and environments.
Bottom Line: Patterns among non-core genes that may drive niche diversification revealed that phosphorus-related gene content reflects source waters rather than host strain used for isolation, and that carbon metabolism genes appear associated with putative mobile elements.However, no clear diagnostic genes emerged to distinguish these phage groups, suggesting blurred boundaries possibly due to cross-infection.Finally, genome-wide comparisons of both diverse and closely related, co-isolated genomes provide a locus-to-locus variability metric that will prove valuable for interpreting metagenomic data sets.
Affiliation: Massachusetts Institute of Technology, Cambridge, MA, USA. email@example.comShow MeSH
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Mentions: To explore genomic diversity among spatially coexisting phages capable of infecting the same host, we included in this sequencing project two phages isolated from the same water sample on the same host strain (Fig. 4A). These two cyanophages, P-HM1 and P-HM2, are highly syntenic and share 200 of 241 and 242 genes, respectively, whose protein sequences are on average 83% identical (Fig. 4A). In contrast, pairwise genome comparisons showed that among the non-co-isolated cyanophages, the genomes share as much as 77–80% of their genes with average identity 72–75% (Fig. 4B) or at the least 22–33% of their genes, with only 48–49% average identity (Fig. 4C).
Affiliation: Massachusetts Institute of Technology, Cambridge, MA, USA. firstname.lastname@example.org