Genomic analysis of oceanic cyanobacterial myoviruses compared with T4-like myoviruses from diverse hosts and environments.
Bottom Line: Patterns among non-core genes that may drive niche diversification revealed that phosphorus-related gene content reflects source waters rather than host strain used for isolation, and that carbon metabolism genes appear associated with putative mobile elements.However, no clear diagnostic genes emerged to distinguish these phage groups, suggesting blurred boundaries possibly due to cross-infection.Finally, genome-wide comparisons of both diverse and closely related, co-isolated genomes provide a locus-to-locus variability metric that will prove valuable for interpreting metagenomic data sets.
Affiliation: Massachusetts Institute of Technology, Cambridge, MA, USA. firstname.lastname@example.orgShow MeSH
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Mentions: In preparation for analyses of gene content and order in the different genomes, we clustered orthologous genes into T4 Gene Clusters (‘T4-GCs’; see Experimental procedures), and used these to define core gene sets common to hierarchical groupings of the genomes (Fig. 2A, see discussion below). A total of 6798 predicted genes in the 26 genomes clustered into 892 T4-GCs, with 1873 genes remaining as singletons.
Affiliation: Massachusetts Institute of Technology, Cambridge, MA, USA. email@example.com