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Visualization of cell-to-cell transmission of mutant huntingtin oligomers.

Herrera F, Tenreiro S, Miller-Fleming L, Outeiro TF - PLoS Curr (2011)

Bottom Line: When huntingtin dimerizes inside the cells, Venus becomes functionally reconstituted and emits fluorescence.We also present evidence that the transmission of huntingtin between cells can be determined in a quantitative manner with our model.Thus, this model can be a powerful screening tool for the identification of modifiers of oligomerization and cell-to-cell traffic of mutant huntingtin.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Medicina Molecular.

ABSTRACT
We developed a new cell model for the visualization of toxic huntingtin oligomers in living cells. Huntingtin exon 1 (25Q or 103Q) was fused to non-fluorescent halves of the Venus protein. When huntingtin dimerizes inside the cells, Venus becomes functionally reconstituted and emits fluorescence. Oligomerization, aggregation and toxicity of mutant huntingtin were assessed by several procedures. We also present evidence that the transmission of huntingtin between cells can be determined in a quantitative manner with our model. Thus, this model can be a powerful screening tool for the identification of modifiers of oligomerization and cell-to-cell traffic of mutant huntingtin.

No MeSH data available.


Related in: MedlinePlus

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Visualization of cell-to-cell transmission of mutant huntingtin oligomers.

Herrera F, Tenreiro S, Miller-Fleming L, Outeiro TF - PLoS Curr (2011)

© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037516&req=5

Bottom Line: When huntingtin dimerizes inside the cells, Venus becomes functionally reconstituted and emits fluorescence.We also present evidence that the transmission of huntingtin between cells can be determined in a quantitative manner with our model.Thus, this model can be a powerful screening tool for the identification of modifiers of oligomerization and cell-to-cell traffic of mutant huntingtin.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Medicina Molecular.

ABSTRACT
We developed a new cell model for the visualization of toxic huntingtin oligomers in living cells. Huntingtin exon 1 (25Q or 103Q) was fused to non-fluorescent halves of the Venus protein. When huntingtin dimerizes inside the cells, Venus becomes functionally reconstituted and emits fluorescence. Oligomerization, aggregation and toxicity of mutant huntingtin were assessed by several procedures. We also present evidence that the transmission of huntingtin between cells can be determined in a quantitative manner with our model. Thus, this model can be a powerful screening tool for the identification of modifiers of oligomerization and cell-to-cell traffic of mutant huntingtin.

No MeSH data available.


Related in: MedlinePlus