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Application of physiologically based modelling and transcriptomics to probe the systems toxicology of aldicarb for Caenorhabditis elegans (Maupas 1900).

Wren JF, Kille P, Spurgeon DJ, Swain S, Sturzenbaum SR, Jager T - Ecotoxicology (2011)

Bottom Line: A clear effect of aldicarb on nematode movement was found suggesting that this pesticide acts as a neurotoxicant.Aldicarb also had an effect on life cycle traits including low concentration life-span extension; high concentration brood size reduction and a high concentration extension of time to first egg.These effects, coupled to the effect on biotransformation enzymes also seen, represent the materialisation of the maintenance costs indicated by DEBtox modelling.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, Cardiff University, Park Place, Cardiff, UK.

ABSTRACT
The toxicity of aldicarb on movement, life cycle, population growth rate and resource allocation, and the gene expression changes underpinning these effects, were investigated for Caenorhabditis elegans. A clear effect of aldicarb on nematode movement was found suggesting that this pesticide acts as a neurotoxicant. Aldicarb also had an effect on life cycle traits including low concentration life-span extension; high concentration brood size reduction and a high concentration extension of time to first egg. All life-cycle and growth data were integrated into a biology-based model (DEBtox) to characterise aldicarb effects on life-history traits, resource allocation and population growth rate within a single modelling framework. The DEBtox fits described concentration dependent effects on individual traits and population growth rate and indicated that the most probable mechanism of action of the pesticide was an increase in energy demands for somatic and reproductive tissue maintenance. Transcriptomic profiling indicated that aldicarb was associated with changes in amino acid metabolism, DNA structure, fatty acid metabolism and cytochrome P450 mediated xenobiotic metabolism. The changes in the amino acid and fatty acid pathways suggest an effect of aldicarb on protein integrity; while effects on DNA suggests that aldicarb influence DNA morphology or replication. Both these effects have the potential to incur increased costs for structural maintenance of macromolecules. These effects, coupled to the effect on biotransformation enzymes also seen, represent the materialisation of the maintenance costs indicated by DEBtox modelling.

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Effects on a mean life-span b mean time to first egg c total brood size and d mean length of the reproductive period for C. elegans exposed individually over the full life-cycle to control or five concentrations of aldicarb, error bars are standard error of mean, asterisks indicate significant differences from controls at: *p < 0.05, ***p < 0.001
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Fig2: Effects on a mean life-span b mean time to first egg c total brood size and d mean length of the reproductive period for C. elegans exposed individually over the full life-cycle to control or five concentrations of aldicarb, error bars are standard error of mean, asterisks indicate significant differences from controls at: *p < 0.05, ***p < 0.001

Mentions: Mean lifespan for control nematodes was 16 days. Exposure to aldicarb significantly affected lifespan (Fig. 2a) (F = 9.37, p < 0.001), resulting in significant extension (37%) at 4 mg/l (Tukey’s post-hoc test, p < 0.001), although not at lower and higher concentrations. Exposure to aldicarb also significantly affected time to first egg whether assessed by ANOVA (F = 15.83, p < 0.001) or the non-parametric Kruskal–Wallis test (H = 43.1, p < 0.001) (Fig. 2b). There was an increase at 16 mg/l compared to all treatments (Tukey’s post-hoc test, p < 0.001) and also an increase at 1 mg/l compared to controls (Tukey’s post-hoc test, p < 0.05).Fig. 2


Application of physiologically based modelling and transcriptomics to probe the systems toxicology of aldicarb for Caenorhabditis elegans (Maupas 1900).

Wren JF, Kille P, Spurgeon DJ, Swain S, Sturzenbaum SR, Jager T - Ecotoxicology (2011)

Effects on a mean life-span b mean time to first egg c total brood size and d mean length of the reproductive period for C. elegans exposed individually over the full life-cycle to control or five concentrations of aldicarb, error bars are standard error of mean, asterisks indicate significant differences from controls at: *p < 0.05, ***p < 0.001
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3037492&req=5

Fig2: Effects on a mean life-span b mean time to first egg c total brood size and d mean length of the reproductive period for C. elegans exposed individually over the full life-cycle to control or five concentrations of aldicarb, error bars are standard error of mean, asterisks indicate significant differences from controls at: *p < 0.05, ***p < 0.001
Mentions: Mean lifespan for control nematodes was 16 days. Exposure to aldicarb significantly affected lifespan (Fig. 2a) (F = 9.37, p < 0.001), resulting in significant extension (37%) at 4 mg/l (Tukey’s post-hoc test, p < 0.001), although not at lower and higher concentrations. Exposure to aldicarb also significantly affected time to first egg whether assessed by ANOVA (F = 15.83, p < 0.001) or the non-parametric Kruskal–Wallis test (H = 43.1, p < 0.001) (Fig. 2b). There was an increase at 16 mg/l compared to all treatments (Tukey’s post-hoc test, p < 0.001) and also an increase at 1 mg/l compared to controls (Tukey’s post-hoc test, p < 0.05).Fig. 2

Bottom Line: A clear effect of aldicarb on nematode movement was found suggesting that this pesticide acts as a neurotoxicant.Aldicarb also had an effect on life cycle traits including low concentration life-span extension; high concentration brood size reduction and a high concentration extension of time to first egg.These effects, coupled to the effect on biotransformation enzymes also seen, represent the materialisation of the maintenance costs indicated by DEBtox modelling.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, Cardiff University, Park Place, Cardiff, UK.

ABSTRACT
The toxicity of aldicarb on movement, life cycle, population growth rate and resource allocation, and the gene expression changes underpinning these effects, were investigated for Caenorhabditis elegans. A clear effect of aldicarb on nematode movement was found suggesting that this pesticide acts as a neurotoxicant. Aldicarb also had an effect on life cycle traits including low concentration life-span extension; high concentration brood size reduction and a high concentration extension of time to first egg. All life-cycle and growth data were integrated into a biology-based model (DEBtox) to characterise aldicarb effects on life-history traits, resource allocation and population growth rate within a single modelling framework. The DEBtox fits described concentration dependent effects on individual traits and population growth rate and indicated that the most probable mechanism of action of the pesticide was an increase in energy demands for somatic and reproductive tissue maintenance. Transcriptomic profiling indicated that aldicarb was associated with changes in amino acid metabolism, DNA structure, fatty acid metabolism and cytochrome P450 mediated xenobiotic metabolism. The changes in the amino acid and fatty acid pathways suggest an effect of aldicarb on protein integrity; while effects on DNA suggests that aldicarb influence DNA morphology or replication. Both these effects have the potential to incur increased costs for structural maintenance of macromolecules. These effects, coupled to the effect on biotransformation enzymes also seen, represent the materialisation of the maintenance costs indicated by DEBtox modelling.

Show MeSH
Related in: MedlinePlus