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Cell origin of human mesenchymal stem cells determines a different healing performance in cardiac regeneration.

Gaebel R, Furlani D, Sorg H, Polchow B, Frank J, Bieback K, Wang W, Klopsch C, Ong LL, Li W, Ma N, Steinhoff G - PLoS ONE (2011)

Bottom Line: Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C).Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C.Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

ABSTRACT
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment of myocardial infarction (MI) remains unexplored. This study was to assess the regenerative potential of hMSC from different origins and to evaluate the role of CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation and received the respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C). Cell survival analyzed by quantitative real time PCR for human GAPDH and capillary density measured by immunostaining showed consistent results. Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C. Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC. Our findings suggests that hMSC originating from different sources showed a different healing performance in cardiac regeneration and CD105(+) hMSC exhibited a favorable survival pattern in infarcted hearts, which translates into a more robust preservation of cardiac function.

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Related in: MedlinePlus

Real time acidification rate as a live cell parameter.CB derived hMSC show significant increased metabolic activity under hypoxic situation compared to BM- and CD105-purified CB-derived hMSC.
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pone-0015652-g008: Real time acidification rate as a live cell parameter.CB derived hMSC show significant increased metabolic activity under hypoxic situation compared to BM- and CD105-purified CB-derived hMSC.

Mentions: As the acidification is closely linked to the cellular energy metabolism, we measured the acidification rate of hMSC under normoxic as well as hypoxic conditions. While under normoxic conditions the cells showed no significant difference (data not shown), CB derived hMSC showed significantly higher metabolic activity than AT-, BM- and CD105-purified CB-hMSC under hypoxic conditions (Figure 8).


Cell origin of human mesenchymal stem cells determines a different healing performance in cardiac regeneration.

Gaebel R, Furlani D, Sorg H, Polchow B, Frank J, Bieback K, Wang W, Klopsch C, Ong LL, Li W, Ma N, Steinhoff G - PLoS ONE (2011)

Real time acidification rate as a live cell parameter.CB derived hMSC show significant increased metabolic activity under hypoxic situation compared to BM- and CD105-purified CB-derived hMSC.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3037376&req=5

pone-0015652-g008: Real time acidification rate as a live cell parameter.CB derived hMSC show significant increased metabolic activity under hypoxic situation compared to BM- and CD105-purified CB-derived hMSC.
Mentions: As the acidification is closely linked to the cellular energy metabolism, we measured the acidification rate of hMSC under normoxic as well as hypoxic conditions. While under normoxic conditions the cells showed no significant difference (data not shown), CB derived hMSC showed significantly higher metabolic activity than AT-, BM- and CD105-purified CB-hMSC under hypoxic conditions (Figure 8).

Bottom Line: Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C).Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C.Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

ABSTRACT
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment of myocardial infarction (MI) remains unexplored. This study was to assess the regenerative potential of hMSC from different origins and to evaluate the role of CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation and received the respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C). Cell survival analyzed by quantitative real time PCR for human GAPDH and capillary density measured by immunostaining showed consistent results. Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C. Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC. Our findings suggests that hMSC originating from different sources showed a different healing performance in cardiac regeneration and CD105(+) hMSC exhibited a favorable survival pattern in infarcted hearts, which translates into a more robust preservation of cardiac function.

Show MeSH
Related in: MedlinePlus