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Cell origin of human mesenchymal stem cells determines a different healing performance in cardiac regeneration.

Gaebel R, Furlani D, Sorg H, Polchow B, Frank J, Bieback K, Wang W, Klopsch C, Ong LL, Li W, Ma N, Steinhoff G - PLoS ONE (2011)

Bottom Line: Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C).Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C.Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

ABSTRACT
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment of myocardial infarction (MI) remains unexplored. This study was to assess the regenerative potential of hMSC from different origins and to evaluate the role of CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation and received the respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C). Cell survival analyzed by quantitative real time PCR for human GAPDH and capillary density measured by immunostaining showed consistent results. Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C. Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC. Our findings suggests that hMSC originating from different sources showed a different healing performance in cardiac regeneration and CD105(+) hMSC exhibited a favorable survival pattern in infarcted hearts, which translates into a more robust preservation of cardiac function.

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Related in: MedlinePlus

Capillary density 6 weeks after MI.A. Representative endothelial CD31 staining at the infarction border zone of level c sections. B. Capillary density in both the RA and the BZ of the LV is significantly higher in MI-AT, MI-BM and MI-CB105+ compared to MIC.
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pone-0015652-g004: Capillary density 6 weeks after MI.A. Representative endothelial CD31 staining at the infarction border zone of level c sections. B. Capillary density in both the RA and the BZ of the LV is significantly higher in MI-AT, MI-BM and MI-CB105+ compared to MIC.

Mentions: The capillary density was determined by CD31-staining 6 weeks after myocardial infarction. Examples of staining from the BZ (border zone) of the infarct area present a lower capillary density in MI-CB treated hearts and the MI-C group. (Figure 4A) Both, at the BZ as well as the RA (remote area), hearts implanted with AT-, BM- and CD105-purified CB-hMSC show a significant higher capillary density compared to hearts which have been treated with cells derived from CB (MI-CB) and the MI-C group, respectively (Figure 4B).


Cell origin of human mesenchymal stem cells determines a different healing performance in cardiac regeneration.

Gaebel R, Furlani D, Sorg H, Polchow B, Frank J, Bieback K, Wang W, Klopsch C, Ong LL, Li W, Ma N, Steinhoff G - PLoS ONE (2011)

Capillary density 6 weeks after MI.A. Representative endothelial CD31 staining at the infarction border zone of level c sections. B. Capillary density in both the RA and the BZ of the LV is significantly higher in MI-AT, MI-BM and MI-CB105+ compared to MIC.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3037376&req=5

pone-0015652-g004: Capillary density 6 weeks after MI.A. Representative endothelial CD31 staining at the infarction border zone of level c sections. B. Capillary density in both the RA and the BZ of the LV is significantly higher in MI-AT, MI-BM and MI-CB105+ compared to MIC.
Mentions: The capillary density was determined by CD31-staining 6 weeks after myocardial infarction. Examples of staining from the BZ (border zone) of the infarct area present a lower capillary density in MI-CB treated hearts and the MI-C group. (Figure 4A) Both, at the BZ as well as the RA (remote area), hearts implanted with AT-, BM- and CD105-purified CB-hMSC show a significant higher capillary density compared to hearts which have been treated with cells derived from CB (MI-CB) and the MI-C group, respectively (Figure 4B).

Bottom Line: Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C).Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C.Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC.

View Article: PubMed Central - PubMed

Affiliation: Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

ABSTRACT
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment of myocardial infarction (MI) remains unexplored. This study was to assess the regenerative potential of hMSC from different origins and to evaluate the role of CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation and received the respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C). Cell survival analyzed by quantitative real time PCR for human GAPDH and capillary density measured by immunostaining showed consistent results. Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C. Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC. Our findings suggests that hMSC originating from different sources showed a different healing performance in cardiac regeneration and CD105(+) hMSC exhibited a favorable survival pattern in infarcted hearts, which translates into a more robust preservation of cardiac function.

Show MeSH
Related in: MedlinePlus