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Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells.

Toh DF, Patel DN, Chan EC, Teo A, Neo SY, Koh HL - Chin Med (2011)

Bottom Line: Panax notoginseng is a potential source of anticancer compounds.Samples of powdered raw P. notoginseng roots were steamed for various durations.Extracts of the raw and steamed samples were subjected to ultra-high pressure liquid chromatography/mass spectrometry (UHPLC-MS) analysis for chemical profiling.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, 117543, Singapore. neo_soek_ying@sics.a-star.edu.sg.

ABSTRACT

Background: Panax notoginseng is a potential source of anticancer compounds. This study aims to investigate the effects of steaming on the chemical profile of P. notoginseng and the anti-proliferative effects of P. notoginseng on liver cancer cells.

Methods: Samples of powdered raw P. notoginseng roots were steamed for various durations. Extracts of the raw and steamed samples were subjected to ultra-high pressure liquid chromatography/mass spectrometry (UHPLC-MS) analysis for chemical profiling. The anti-proliferative effects on three human liver cancer cells, namely SNU449, SNU182 and HepG2, were evaluated using colorimetric WST-1 assay.

Results: Steaming changed chromatographic and pharmacological profiles of P. notoginseng, causing differences in activities such as inhibition of cancer growth. Steamed P. notoginseng exhibited greater anti-proliferative effects against liver cancer cells (SNU449, SNU182 and HepG2) than its raw form; steaming up to 24 hours increased bioactivities. Steaming increased the concentrations of ginsenoside Rh2, Rk1, Rk3 and 20S-Rg3 and enhanced growth inhibition of liver cancer cells.

Conclusion: Steaming changes the chemical profile as well as anti-cancer biological activities of P. notoginseng. Steamed P. notoginseng contains potential compounds for the treatment of liver cancer.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of some ginsenosides and notoginsenoside in P. notoginseng. In raw P. notoginseng: notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and Rd. In steamed P. notoginseng: 20S-Rh1, 20R-Rh1, 20S-Rg3, 20R-Rg3, Rh2, Rg5, Rh4, Rk1 and Rk3. Glc: glucose; Xyl: xylose; Rha: rhamnose
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Figure 3: Chemical structures of some ginsenosides and notoginsenoside in P. notoginseng. In raw P. notoginseng: notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and Rd. In steamed P. notoginseng: 20S-Rh1, 20R-Rh1, 20S-Rg3, 20R-Rg3, Rh2, Rg5, Rh4, Rk1 and Rk3. Glc: glucose; Xyl: xylose; Rha: rhamnose

Mentions: To identify the active components responsible for the anti-proliferative activities of P. notoginseng, we screened ginsenosides enriched in steamed P. notoginseng, namely 20S-Rh1, Rk3, Rk1, 20S-Rg3, Rh2 and 20R-Rh1. Ginsenosides Rg1, Rb1, Rd, Re and notoginsenoside R1 which predominate in raw P. notoginseng were also assessed for comparison. The structures of these ginsenosides and notoginsenoside are shown in Figure 3.


Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells.

Toh DF, Patel DN, Chan EC, Teo A, Neo SY, Koh HL - Chin Med (2011)

Chemical structures of some ginsenosides and notoginsenoside in P. notoginseng. In raw P. notoginseng: notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and Rd. In steamed P. notoginseng: 20S-Rh1, 20R-Rh1, 20S-Rg3, 20R-Rg3, Rh2, Rg5, Rh4, Rk1 and Rk3. Glc: glucose; Xyl: xylose; Rha: rhamnose
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3037355&req=5

Figure 3: Chemical structures of some ginsenosides and notoginsenoside in P. notoginseng. In raw P. notoginseng: notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and Rd. In steamed P. notoginseng: 20S-Rh1, 20R-Rh1, 20S-Rg3, 20R-Rg3, Rh2, Rg5, Rh4, Rk1 and Rk3. Glc: glucose; Xyl: xylose; Rha: rhamnose
Mentions: To identify the active components responsible for the anti-proliferative activities of P. notoginseng, we screened ginsenosides enriched in steamed P. notoginseng, namely 20S-Rh1, Rk3, Rk1, 20S-Rg3, Rh2 and 20R-Rh1. Ginsenosides Rg1, Rb1, Rd, Re and notoginsenoside R1 which predominate in raw P. notoginseng were also assessed for comparison. The structures of these ginsenosides and notoginsenoside are shown in Figure 3.

Bottom Line: Panax notoginseng is a potential source of anticancer compounds.Samples of powdered raw P. notoginseng roots were steamed for various durations.Extracts of the raw and steamed samples were subjected to ultra-high pressure liquid chromatography/mass spectrometry (UHPLC-MS) analysis for chemical profiling.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, 117543, Singapore. neo_soek_ying@sics.a-star.edu.sg.

ABSTRACT

Background: Panax notoginseng is a potential source of anticancer compounds. This study aims to investigate the effects of steaming on the chemical profile of P. notoginseng and the anti-proliferative effects of P. notoginseng on liver cancer cells.

Methods: Samples of powdered raw P. notoginseng roots were steamed for various durations. Extracts of the raw and steamed samples were subjected to ultra-high pressure liquid chromatography/mass spectrometry (UHPLC-MS) analysis for chemical profiling. The anti-proliferative effects on three human liver cancer cells, namely SNU449, SNU182 and HepG2, were evaluated using colorimetric WST-1 assay.

Results: Steaming changed chromatographic and pharmacological profiles of P. notoginseng, causing differences in activities such as inhibition of cancer growth. Steamed P. notoginseng exhibited greater anti-proliferative effects against liver cancer cells (SNU449, SNU182 and HepG2) than its raw form; steaming up to 24 hours increased bioactivities. Steaming increased the concentrations of ginsenoside Rh2, Rk1, Rk3 and 20S-Rg3 and enhanced growth inhibition of liver cancer cells.

Conclusion: Steaming changes the chemical profile as well as anti-cancer biological activities of P. notoginseng. Steamed P. notoginseng contains potential compounds for the treatment of liver cancer.

No MeSH data available.


Related in: MedlinePlus