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Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules.

Giang do T, Hoang VD - J Young Pharm (2010)

Bottom Line: Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules.For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied.The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy 13-15 Le Thanh Tong, Hanoi, Vietnam.

ABSTRACT
Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin. Based on the statistical comparison of spectrophotometric and chromatographic data, the interchangeability between HPLC and UV spectrophotometric techniques has been suggested for the routine analysis.

No MeSH data available.


Chemical structures of (a) Amoxicillin trihydrate and (b) Cloxacillin sodium
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Figure 0001: Chemical structures of (a) Amoxicillin trihydrate and (b) Cloxacillin sodium

Mentions: Amoxicillin, formerly amoxycillin [Figure 1 a], is a moderate-spectrum beta-lactam antibiotic used to treat infections caused by penicillin-sensitive gram-positive bacteria as well as some gram-negative bacteria.[1] Amoxicillin is resistant to inactivation by gastric acid. It is usually the drug of choice because it is more rapidly and more completely absorbed than other beta-lactam antibiotics when orally administered. To overcome its sensitivity to destruction by beta-lactamases, amoxicillin has been co-administered with clavulanic acid, a potent betalactamase inhibitor[2] in pharmaceutical preparations.


Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules.

Giang do T, Hoang VD - J Young Pharm (2010)

Chemical structures of (a) Amoxicillin trihydrate and (b) Cloxacillin sodium
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3021696&req=5

Figure 0001: Chemical structures of (a) Amoxicillin trihydrate and (b) Cloxacillin sodium
Mentions: Amoxicillin, formerly amoxycillin [Figure 1 a], is a moderate-spectrum beta-lactam antibiotic used to treat infections caused by penicillin-sensitive gram-positive bacteria as well as some gram-negative bacteria.[1] Amoxicillin is resistant to inactivation by gastric acid. It is usually the drug of choice because it is more rapidly and more completely absorbed than other beta-lactam antibiotics when orally administered. To overcome its sensitivity to destruction by beta-lactamases, amoxicillin has been co-administered with clavulanic acid, a potent betalactamase inhibitor[2] in pharmaceutical preparations.

Bottom Line: Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules.For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied.The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy 13-15 Le Thanh Tong, Hanoi, Vietnam.

ABSTRACT
Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin. Based on the statistical comparison of spectrophotometric and chromatographic data, the interchangeability between HPLC and UV spectrophotometric techniques has been suggested for the routine analysis.

No MeSH data available.