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Glucagon Secreting Cells Responds to Insulin Secretion In vitro Using Immunocytochemistry.

Aswar M, Aswar U, Subhedar N - J Young Pharm (2010)

Bottom Line: In the present study, pancreas of rats were dissected and transferred to HEPES buffer (25 mM, pH 7.4).In the sections of control tissue, the Glucagon Immunoreactive Cells (GIC) were distinctly visible; on average 40-50 cells were counted in each islet.However in vitro treatment with 10 nm insulin caused 285.89 % increase in the GIC and was found to be highly significant (P< 0.001).

View Article: PubMed Central - PubMed

Affiliation: Sinhgad Institute of Pharmacy, Narhe, Pune-41, India.

ABSTRACT

Unlabelled: In the present study, pancreas of rats were dissected and transferred to HEPES buffer (25 mM, pH 7.4). The control tissue pieces were kept in culture medium for one hour and the treated tissues were kept in same medium for 30 minutes and incubated with Insulin (10 nm and 100 nm) for another half hour, then tissues were transferred to Bouin's fixative (overnight at 40 ° Cc), cryosectioned (15 µm at -16 0 c) and subjected to immunocytochemical labeling with antibodies against Glucagon.

Results: In the sections of control tissue, the Glucagon Immunoreactive Cells (GIC) were distinctly visible; on average 40-50 cells were counted in each islet. However in vitro treatment with 10 nm insulin caused 285.89 % increase in the GIC and was found to be highly significant (P< 0.001). Whereas in 100 nm Insulin treatment, 206.41% increase in GIC was seen, this was significant with the control but non-significant with 10 nm Insulin treatment.

No MeSH data available.


Related in: MedlinePlus

Effect of insulin treatment on the population of glucagon secreting cells. Statistical analysis was carried out using one way ANOVA followed by Tukey-Kramer multiple comparison test. **indicates p<0.001 compared to control, ns indicates p>0.05 compared to 10 nM insulin treatment.
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Figure 0001: Effect of insulin treatment on the population of glucagon secreting cells. Statistical analysis was carried out using one way ANOVA followed by Tukey-Kramer multiple comparison test. **indicates p<0.001 compared to control, ns indicates p>0.05 compared to 10 nM insulin treatment.

Mentions: The results of the effect of insulin on the population of glucagon are summarized in Figure 1. In the sections of control tissue, a limited number of glucagon immunoreactive cells were seen along the periphery of islets of Langerhans [Figure 2]. While the treatment with 10 nM insulin caused a 285.89 % increase in the number of glucagon immunoreactive cells [Figure 3], which was found to be highly significant (P< 0.001), 206.41% increase was observed following insulin treatment at 100 nM [Figure 4]. This change was also found to be highly significant as compared to control. However, no significant difference was found between cell population in the tissue samples treated with 10 nM and 100 nM concentration of insulin.


Glucagon Secreting Cells Responds to Insulin Secretion In vitro Using Immunocytochemistry.

Aswar M, Aswar U, Subhedar N - J Young Pharm (2010)

Effect of insulin treatment on the population of glucagon secreting cells. Statistical analysis was carried out using one way ANOVA followed by Tukey-Kramer multiple comparison test. **indicates p<0.001 compared to control, ns indicates p>0.05 compared to 10 nM insulin treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3021688&req=5

Figure 0001: Effect of insulin treatment on the population of glucagon secreting cells. Statistical analysis was carried out using one way ANOVA followed by Tukey-Kramer multiple comparison test. **indicates p<0.001 compared to control, ns indicates p>0.05 compared to 10 nM insulin treatment.
Mentions: The results of the effect of insulin on the population of glucagon are summarized in Figure 1. In the sections of control tissue, a limited number of glucagon immunoreactive cells were seen along the periphery of islets of Langerhans [Figure 2]. While the treatment with 10 nM insulin caused a 285.89 % increase in the number of glucagon immunoreactive cells [Figure 3], which was found to be highly significant (P< 0.001), 206.41% increase was observed following insulin treatment at 100 nM [Figure 4]. This change was also found to be highly significant as compared to control. However, no significant difference was found between cell population in the tissue samples treated with 10 nM and 100 nM concentration of insulin.

Bottom Line: In the present study, pancreas of rats were dissected and transferred to HEPES buffer (25 mM, pH 7.4).In the sections of control tissue, the Glucagon Immunoreactive Cells (GIC) were distinctly visible; on average 40-50 cells were counted in each islet.However in vitro treatment with 10 nm insulin caused 285.89 % increase in the GIC and was found to be highly significant (P< 0.001).

View Article: PubMed Central - PubMed

Affiliation: Sinhgad Institute of Pharmacy, Narhe, Pune-41, India.

ABSTRACT

Unlabelled: In the present study, pancreas of rats were dissected and transferred to HEPES buffer (25 mM, pH 7.4). The control tissue pieces were kept in culture medium for one hour and the treated tissues were kept in same medium for 30 minutes and incubated with Insulin (10 nm and 100 nm) for another half hour, then tissues were transferred to Bouin's fixative (overnight at 40 ° Cc), cryosectioned (15 µm at -16 0 c) and subjected to immunocytochemical labeling with antibodies against Glucagon.

Results: In the sections of control tissue, the Glucagon Immunoreactive Cells (GIC) were distinctly visible; on average 40-50 cells were counted in each islet. However in vitro treatment with 10 nm insulin caused 285.89 % increase in the GIC and was found to be highly significant (P< 0.001). Whereas in 100 nm Insulin treatment, 206.41% increase in GIC was seen, this was significant with the control but non-significant with 10 nm Insulin treatment.

No MeSH data available.


Related in: MedlinePlus