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Tissue factor expression in human pterygium.

Ando R, Kase S, Ohashi T, Dong Z, Fukuhara J, Kanda A, Murata M, Noda K, Kitaichi N, Ishida S - Mol. Vis. (2011)

Bottom Line: The number of TF-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival epithelial cells (p<0.001).TF immunoreactivity was detected in α-SMA-positive or -negative pterygial epithelial cells.EGFR immunoreactivity was detected in pterygial epithelium, which was colocalized with TF.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

ABSTRACT

Purpose: A pterygium shows tumor-like characteristics, such as proliferation, invasion, and epithelial-mesenchymal transition (EMT). Previous reports suggest that tissue factor (TF) expression is closely related to the EMT of tumor cells, and subsequent tumor development. In this study, we analyzed the expression and immunolocalization of TF in pterygial and normal conjunctival tissues of humans.

Methods: Eight pterygia and three normal bulbar conjunctivas, surgically removed, were used in this study. Formalin-fixed, paraffin-embedded tissues were submitted for immunohistochemical analysis with anti-TF antibody. Double staining immunohistochemistry was performed to assess TF and alpha-smooth muscle actin (α-SMA) or epidermal growth factor receptor (EGFR) expression in the pterygia.

Results: Immunoreactivity for TF was detected in all pterygial tissues examined. TF immunoreactivity was localized in the cytoplasm of basal, suprabasal, and superficial epithelial cells. The number of TF-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival epithelial cells (p<0.001). TF immunoreactivity was detected in α-SMA-positive or -negative pterygial epithelial cells. EGFR immunoreactivity was detected in pterygial epithelium, which was colocalized with TF.

Conclusions: These results suggest that TF plays a potential role in the pathogenesis and development of a pterygium, and that TF expression might be involved through EMT-dependent and -independent pathways.

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Related in: MedlinePlus

Double staining immunohistochemistry was performed for TF (green) and EGFR (red) in pterygial tissue. Nuclear staining and TF immunoreactivity are shown in A and B, respectively. C, D: EGFR immunoreactivity was observed broadly in pterygial epithelial cells. The scale bar represents 50 μm.
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f4: Double staining immunohistochemistry was performed for TF (green) and EGFR (red) in pterygial tissue. Nuclear staining and TF immunoreactivity are shown in A and B, respectively. C, D: EGFR immunoreactivity was observed broadly in pterygial epithelial cells. The scale bar represents 50 μm.

Mentions: Double staining immunohistochemistry for TF and EGFR was also performed in pterygial tissue. EGFR immunoreactivity was observed in pterygial epithelial cells, which was colocalized with TF in preferentially basal cells (Figure 4).


Tissue factor expression in human pterygium.

Ando R, Kase S, Ohashi T, Dong Z, Fukuhara J, Kanda A, Murata M, Noda K, Kitaichi N, Ishida S - Mol. Vis. (2011)

Double staining immunohistochemistry was performed for TF (green) and EGFR (red) in pterygial tissue. Nuclear staining and TF immunoreactivity are shown in A and B, respectively. C, D: EGFR immunoreactivity was observed broadly in pterygial epithelial cells. The scale bar represents 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3021580&req=5

f4: Double staining immunohistochemistry was performed for TF (green) and EGFR (red) in pterygial tissue. Nuclear staining and TF immunoreactivity are shown in A and B, respectively. C, D: EGFR immunoreactivity was observed broadly in pterygial epithelial cells. The scale bar represents 50 μm.
Mentions: Double staining immunohistochemistry for TF and EGFR was also performed in pterygial tissue. EGFR immunoreactivity was observed in pterygial epithelial cells, which was colocalized with TF in preferentially basal cells (Figure 4).

Bottom Line: The number of TF-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival epithelial cells (p<0.001).TF immunoreactivity was detected in α-SMA-positive or -negative pterygial epithelial cells.EGFR immunoreactivity was detected in pterygial epithelium, which was colocalized with TF.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

ABSTRACT

Purpose: A pterygium shows tumor-like characteristics, such as proliferation, invasion, and epithelial-mesenchymal transition (EMT). Previous reports suggest that tissue factor (TF) expression is closely related to the EMT of tumor cells, and subsequent tumor development. In this study, we analyzed the expression and immunolocalization of TF in pterygial and normal conjunctival tissues of humans.

Methods: Eight pterygia and three normal bulbar conjunctivas, surgically removed, were used in this study. Formalin-fixed, paraffin-embedded tissues were submitted for immunohistochemical analysis with anti-TF antibody. Double staining immunohistochemistry was performed to assess TF and alpha-smooth muscle actin (α-SMA) or epidermal growth factor receptor (EGFR) expression in the pterygia.

Results: Immunoreactivity for TF was detected in all pterygial tissues examined. TF immunoreactivity was localized in the cytoplasm of basal, suprabasal, and superficial epithelial cells. The number of TF-immunopositive cells in pterygial epithelial cells was significantly higher than in normal conjunctival epithelial cells (p<0.001). TF immunoreactivity was detected in α-SMA-positive or -negative pterygial epithelial cells. EGFR immunoreactivity was detected in pterygial epithelium, which was colocalized with TF.

Conclusions: These results suggest that TF plays a potential role in the pathogenesis and development of a pterygium, and that TF expression might be involved through EMT-dependent and -independent pathways.

Show MeSH
Related in: MedlinePlus