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Species difference of CD137 ligand signaling in human and murine monocytes.

Tang Q, Jiang D, Shao Z, Martínez Gómez JM, Schwarz H - PLoS ONE (2011)

Bottom Line: To allow an in vivo evaluation of the potency of CD137L-DCs in murine models we aimed at generating murine CD137L-DCs.But CD137 ligand signaling did not induce expression of inflammatory cytokines and costimulatory molecules in murine monocytes and these cells had no T cell stimulatory activity.Furthermore, while CD137 ligand signaling induces maturation of human immature classical DCs it failed to do so with murine immature classical DCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, and Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

ABSTRACT

Background: Stimulation of CD137 ligand on human monocytes has been shown to induce DC differentiation, and these CD137L-DCs are more potent than classical DCs, in stimulating T cell responses in vitro. To allow an in vivo evaluation of the potency of CD137L-DCs in murine models we aimed at generating murine CD137L-DCs.

Methodology/principal findings: When stimulated through CD137 ligand murine monocytes responded just as human monocytes with an increased adherence, morphological changes, proliferation and an increase in viable cell numbers. But CD137 ligand signaling did not induce expression of inflammatory cytokines and costimulatory molecules in murine monocytes and these cells had no T cell stimulatory activity. Murine monocytes did not differentiate to inflammatory DCs upon CD137 ligand signaling. Furthermore, while CD137 ligand signaling induces maturation of human immature classical DCs it failed to do so with murine immature classical DCs.

Conclusions/significance: These data demonstrate that both human and murine monocytes become activated by CD137 ligand signaling but only human and not murine monocytes differentiate to inflammatory DCs.

Show MeSH
Activation of murine monocytes by recombinant CD137 protein.Monocytes were cultured under indicated conditions for 7 days. (A) Morphological changes were documented by photography at 20x (upper panel) and 63x (lower panel) magnifications. (B) Numbers of viable cells were determined by flow cytometry using Sphero Accucount Blank Particles. (C) Proliferation was determined by 3H-thymidine incorporation at 3 day of culture. *: p<0.05. Data are representative of three independent experiments.
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pone-0016129-g001: Activation of murine monocytes by recombinant CD137 protein.Monocytes were cultured under indicated conditions for 7 days. (A) Morphological changes were documented by photography at 20x (upper panel) and 63x (lower panel) magnifications. (B) Numbers of viable cells were determined by flow cytometry using Sphero Accucount Blank Particles. (C) Proliferation was determined by 3H-thymidine incorporation at 3 day of culture. *: p<0.05. Data are representative of three independent experiments.

Mentions: In response to CD137 ligand signaling the cells attached to the plates, spread and formed lamellipodia (Figure 1A), similar to what has been shown for human monocytes [7]. In contrast, classical DCs induced by GM-CSF + IL-4 showed formed clusters and a substantial proportion of the cells remained in suspension. Of the Fc-treated control monocytes only few cells attached to the plate, and majority of the attached cells lacked lamellipodia formation.


Species difference of CD137 ligand signaling in human and murine monocytes.

Tang Q, Jiang D, Shao Z, Martínez Gómez JM, Schwarz H - PLoS ONE (2011)

Activation of murine monocytes by recombinant CD137 protein.Monocytes were cultured under indicated conditions for 7 days. (A) Morphological changes were documented by photography at 20x (upper panel) and 63x (lower panel) magnifications. (B) Numbers of viable cells were determined by flow cytometry using Sphero Accucount Blank Particles. (C) Proliferation was determined by 3H-thymidine incorporation at 3 day of culture. *: p<0.05. Data are representative of three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3021528&req=5

pone-0016129-g001: Activation of murine monocytes by recombinant CD137 protein.Monocytes were cultured under indicated conditions for 7 days. (A) Morphological changes were documented by photography at 20x (upper panel) and 63x (lower panel) magnifications. (B) Numbers of viable cells were determined by flow cytometry using Sphero Accucount Blank Particles. (C) Proliferation was determined by 3H-thymidine incorporation at 3 day of culture. *: p<0.05. Data are representative of three independent experiments.
Mentions: In response to CD137 ligand signaling the cells attached to the plates, spread and formed lamellipodia (Figure 1A), similar to what has been shown for human monocytes [7]. In contrast, classical DCs induced by GM-CSF + IL-4 showed formed clusters and a substantial proportion of the cells remained in suspension. Of the Fc-treated control monocytes only few cells attached to the plate, and majority of the attached cells lacked lamellipodia formation.

Bottom Line: To allow an in vivo evaluation of the potency of CD137L-DCs in murine models we aimed at generating murine CD137L-DCs.But CD137 ligand signaling did not induce expression of inflammatory cytokines and costimulatory molecules in murine monocytes and these cells had no T cell stimulatory activity.Furthermore, while CD137 ligand signaling induces maturation of human immature classical DCs it failed to do so with murine immature classical DCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, and Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

ABSTRACT

Background: Stimulation of CD137 ligand on human monocytes has been shown to induce DC differentiation, and these CD137L-DCs are more potent than classical DCs, in stimulating T cell responses in vitro. To allow an in vivo evaluation of the potency of CD137L-DCs in murine models we aimed at generating murine CD137L-DCs.

Methodology/principal findings: When stimulated through CD137 ligand murine monocytes responded just as human monocytes with an increased adherence, morphological changes, proliferation and an increase in viable cell numbers. But CD137 ligand signaling did not induce expression of inflammatory cytokines and costimulatory molecules in murine monocytes and these cells had no T cell stimulatory activity. Murine monocytes did not differentiate to inflammatory DCs upon CD137 ligand signaling. Furthermore, while CD137 ligand signaling induces maturation of human immature classical DCs it failed to do so with murine immature classical DCs.

Conclusions/significance: These data demonstrate that both human and murine monocytes become activated by CD137 ligand signaling but only human and not murine monocytes differentiate to inflammatory DCs.

Show MeSH