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Molecular analysis of echovirus 13 isolates and aseptic meningitis, Spain.

Avellón A, Casas I, Trallero G, Pérez C, Tenorio A, Palacios G - Emerging Infect. Dis. (2003)

Bottom Line: The isolates from other countries obtained before 2000 were genetically distant.Changes in EV13 coding sequence involved several differences in the C-terminal extreme of the VP1 protein.Part of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Salud Carlos III, Madrid, Spain. aavellon@isciii.es

ABSTRACT
Echovirus 13 (EV13), considered rare, was reported worldwide in 2000, mostly related to aseptic meningitis outbreaks. In Spain, 135 EV13 isolates were identified. The genetic relationships between 64 representative strains from Spain and other reported isolates from the United States, Germany, Italy, Japan, and Sweden were described by analyzing the partial sequence of the major capsid protein (VP1) gene. The strains from Spain were clearly identified as EV13 (79.5% similarity with the EV13 reference strain) and were grouped phylogenetically into two different clusters (by origination on either the Iberian Peninsula or Canary Islands). Isolates from Germany from 2000 clustered with the Canary Islands group. The isolates from other countries obtained before 2000 were genetically distant. Changes in EV13 coding sequence involved several differences in the C-terminal extreme of the VP1 protein. Part of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus.

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Histogram of the observed distances (Kimura two parameters method) within Canary Islands and Iberian Peninsula groups (dark gray) and between them (light gray). Analysis of variance (ANOVA) (one-way) test results: F=5238 (p=0.000). Distances within homologous groups: Canary Islands (light gray): mean 0.008 (standard deviation [SD] 0.004; n=351); Iberian Peninsula (dark gray): mean 0.010 (SD0.006; n=666). Distances between heterologous groups (black): mean 0.033 (SD 0.005; n=999).
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Figure 3: Histogram of the observed distances (Kimura two parameters method) within Canary Islands and Iberian Peninsula groups (dark gray) and between them (light gray). Analysis of variance (ANOVA) (one-way) test results: F=5238 (p=0.000). Distances within homologous groups: Canary Islands (light gray): mean 0.008 (standard deviation [SD] 0.004; n=351); Iberian Peninsula (dark gray): mean 0.010 (SD0.006; n=666). Distances between heterologous groups (black): mean 0.033 (SD 0.005; n=999).

Mentions: The phylogenetic tree in Figure 2 was reconstructed through the neighbor-joining method (MEGA version 2.1 software package; available from URL: http://www.magasoftware.net) by using Kimura two parameters as substitution model, with statistical significance of phylogenies estimated by bootstrap analysis with 1,000 pseudoreplicate datasets. The transition-transversion ratio employed (TTRatio=2) was estimated through the Tree-puzzle version 5.0 software (available from: URL: http://www.tree-puzzle.de). Genetic distances were calculated using the same model of nucleotide substitution. The distance matrix was recorded and the pairwise observed distance values were employed to define different subgroups and to build the histogram in Figure 3.. The analysis of variance one-way test, with a statistical significance value of 0.05, was used to compare the means of the pairwise observed distance value matrix. To reconstruct the phylogenetic tree shown in Figure 4, the maximum-likelihood method (DNAML program, PHYLIP software [available from: URL: http://evolution.genetics.washington.edu]), was used, with hidden Markov as the model of nt substitution. A Poisson correction model was used to compare the amino acid sequences.


Molecular analysis of echovirus 13 isolates and aseptic meningitis, Spain.

Avellón A, Casas I, Trallero G, Pérez C, Tenorio A, Palacios G - Emerging Infect. Dis. (2003)

Histogram of the observed distances (Kimura two parameters method) within Canary Islands and Iberian Peninsula groups (dark gray) and between them (light gray). Analysis of variance (ANOVA) (one-way) test results: F=5238 (p=0.000). Distances within homologous groups: Canary Islands (light gray): mean 0.008 (standard deviation [SD] 0.004; n=351); Iberian Peninsula (dark gray): mean 0.010 (SD0.006; n=666). Distances between heterologous groups (black): mean 0.033 (SD 0.005; n=999).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3020609&req=5

Figure 3: Histogram of the observed distances (Kimura two parameters method) within Canary Islands and Iberian Peninsula groups (dark gray) and between them (light gray). Analysis of variance (ANOVA) (one-way) test results: F=5238 (p=0.000). Distances within homologous groups: Canary Islands (light gray): mean 0.008 (standard deviation [SD] 0.004; n=351); Iberian Peninsula (dark gray): mean 0.010 (SD0.006; n=666). Distances between heterologous groups (black): mean 0.033 (SD 0.005; n=999).
Mentions: The phylogenetic tree in Figure 2 was reconstructed through the neighbor-joining method (MEGA version 2.1 software package; available from URL: http://www.magasoftware.net) by using Kimura two parameters as substitution model, with statistical significance of phylogenies estimated by bootstrap analysis with 1,000 pseudoreplicate datasets. The transition-transversion ratio employed (TTRatio=2) was estimated through the Tree-puzzle version 5.0 software (available from: URL: http://www.tree-puzzle.de). Genetic distances were calculated using the same model of nucleotide substitution. The distance matrix was recorded and the pairwise observed distance values were employed to define different subgroups and to build the histogram in Figure 3.. The analysis of variance one-way test, with a statistical significance value of 0.05, was used to compare the means of the pairwise observed distance value matrix. To reconstruct the phylogenetic tree shown in Figure 4, the maximum-likelihood method (DNAML program, PHYLIP software [available from: URL: http://evolution.genetics.washington.edu]), was used, with hidden Markov as the model of nt substitution. A Poisson correction model was used to compare the amino acid sequences.

Bottom Line: The isolates from other countries obtained before 2000 were genetically distant.Changes in EV13 coding sequence involved several differences in the C-terminal extreme of the VP1 protein.Part of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Salud Carlos III, Madrid, Spain. aavellon@isciii.es

ABSTRACT
Echovirus 13 (EV13), considered rare, was reported worldwide in 2000, mostly related to aseptic meningitis outbreaks. In Spain, 135 EV13 isolates were identified. The genetic relationships between 64 representative strains from Spain and other reported isolates from the United States, Germany, Italy, Japan, and Sweden were described by analyzing the partial sequence of the major capsid protein (VP1) gene. The strains from Spain were clearly identified as EV13 (79.5% similarity with the EV13 reference strain) and were grouped phylogenetically into two different clusters (by origination on either the Iberian Peninsula or Canary Islands). Isolates from Germany from 2000 clustered with the Canary Islands group. The isolates from other countries obtained before 2000 were genetically distant. Changes in EV13 coding sequence involved several differences in the C-terminal extreme of the VP1 protein. Part of the neutralizing antigenic site III has been described in this genome region in poliovirus and swine vesicular disease virus.

Show MeSH
Related in: MedlinePlus