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RNA viruses in hymenopteran pollinators: evidence of inter-Taxa virus transmission via pollen and potential impact on non-Apis hymenopteran species.

Singh R, Levitt AL, Rajotte EG, Holmes EC, Ostiguy N, Vanengelsdorp D, Lipkin WI, Depamphilis CW, Toth AL, Cox-Foster DL - PLoS ONE (2010)

Bottom Line: Although overall pollinator populations have declined over the last couple of decades, the honey bee (Apis mellifera) malady, colony collapse disorder (CCD), has caused major concern in the agricultural community.This finding further expands the viral host range and implies a possible deeper impact on the health of our ecosystem.Notably, in cases where honey bee apiaries affected by CCD harbored honey bees with Israeli Acute Paralysis virus (IAPV), nearby non-Apis hymenopteran pollinators also had IAPV, while those near apiaries without IAPV did not.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, The Pennsylvania State University, Pennsylvania, United States of America.

ABSTRACT
Although overall pollinator populations have declined over the last couple of decades, the honey bee (Apis mellifera) malady, colony collapse disorder (CCD), has caused major concern in the agricultural community. Among honey bee pathogens, RNA viruses are emerging as a serious threat and are suspected as major contributors to CCD. Recent detection of these viral species in bumble bees suggests a possible wider environmental spread of these viruses with potential broader impact. It is therefore vital to study the ecology and epidemiology of these viruses in the hymenopteran pollinator community as a whole. We studied the viral distribution in honey bees, in their pollen loads, and in other non-Apis hymenopteran pollinators collected from flowering plants in Pennsylvania, New York, and Illinois in the United States. Viruses in the samples were detected using reverse transcriptase-PCR and confirmed by sequencing. For the first time, we report the molecular detection of picorna-like RNA viruses (deformed wing virus, sacbrood virus and black queen cell virus) in pollen pellets collected directly from forager bees. Pollen pellets from several uninfected forager bees were detected with virus, indicating that pollen itself may harbor viruses. The viruses in the pollen and honey stored in the hive were demonstrated to be infective, with the queen becoming infected and laying infected eggs after these virus-contaminated foods were given to virus-free colonies. These viruses were detected in eleven other non-Apis hymenopteran species, ranging from many solitary bees to bumble bees and wasps. This finding further expands the viral host range and implies a possible deeper impact on the health of our ecosystem. Phylogenetic analyses support that these viruses are disseminating freely among the pollinators via the flower pollen itself. Notably, in cases where honey bee apiaries affected by CCD harbored honey bees with Israeli Acute Paralysis virus (IAPV), nearby non-Apis hymenopteran pollinators also had IAPV, while those near apiaries without IAPV did not. In containment greenhouse experiments, IAPV moved from infected honey bees to bumble bees and from infected bumble bees to honey bees within a week, demonstrating that the viruses could be transmitted from one species to another. This study adds to our present understanding of virus epidemiology and may help explain bee disease patterns and pollinator population decline in general.

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Phylogenetic comparison of IAPV sequences detected in honeybees and non-Apis hymenopteran pollinators collected near IAPV(+) apiaries.An unrooted maximum likelihood phylogenetic tree of IAPV (based on 771-nt for capsid region) was generated using a region of the structural proteins of the virus. The support for the indicated branching topology was evaluated by using bootstrap re-sampling of the sequences 1,000 times. Nodes supported by bootstrap values over 70 are given. Strains were annotated by genus, species, identification label, country of isolation and year of isolation. Green =  virus from non-Apis hymenopteran pollinators; Black  =  virus sequences from original isolation and honey bees from CCD-affected operations [16]. Non-Apis hymenopteran pollinators collected from same local are indicated by common symbol following sample label.
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pone-0014357-g007: Phylogenetic comparison of IAPV sequences detected in honeybees and non-Apis hymenopteran pollinators collected near IAPV(+) apiaries.An unrooted maximum likelihood phylogenetic tree of IAPV (based on 771-nt for capsid region) was generated using a region of the structural proteins of the virus. The support for the indicated branching topology was evaluated by using bootstrap re-sampling of the sequences 1,000 times. Nodes supported by bootstrap values over 70 are given. Strains were annotated by genus, species, identification label, country of isolation and year of isolation. Green =  virus from non-Apis hymenopteran pollinators; Black  =  virus sequences from original isolation and honey bees from CCD-affected operations [16]. Non-Apis hymenopteran pollinators collected from same local are indicated by common symbol following sample label.

Mentions: Similarly, for IAPV, an ML phylogenetic tree was inferred using a part of the structural polyprotein for the virus from the Apis mellifera specimens taken from apiaries diagnosed with CCD [16], [17] and the non-Apis pollinators collected from near two of the IAPV-infected apiaries (associated with Operation 3 [16]). The IAPV detected in the non-Apis pollinators did not cluster separately from the IAPV in the honey bees in nearby apiaries (Figure 7) (AI, P = 0.083; PS, P = 1). Interestingly, the viral sequences from non-Apis hymenopteran pollinators collected from the two apiaries segregated by sampling location, with the non-Apis hymenopteran pollinators from each site possessing a clearly phylogenetically distinct lineage of IAPV.


RNA viruses in hymenopteran pollinators: evidence of inter-Taxa virus transmission via pollen and potential impact on non-Apis hymenopteran species.

Singh R, Levitt AL, Rajotte EG, Holmes EC, Ostiguy N, Vanengelsdorp D, Lipkin WI, Depamphilis CW, Toth AL, Cox-Foster DL - PLoS ONE (2010)

Phylogenetic comparison of IAPV sequences detected in honeybees and non-Apis hymenopteran pollinators collected near IAPV(+) apiaries.An unrooted maximum likelihood phylogenetic tree of IAPV (based on 771-nt for capsid region) was generated using a region of the structural proteins of the virus. The support for the indicated branching topology was evaluated by using bootstrap re-sampling of the sequences 1,000 times. Nodes supported by bootstrap values over 70 are given. Strains were annotated by genus, species, identification label, country of isolation and year of isolation. Green =  virus from non-Apis hymenopteran pollinators; Black  =  virus sequences from original isolation and honey bees from CCD-affected operations [16]. Non-Apis hymenopteran pollinators collected from same local are indicated by common symbol following sample label.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3008715&req=5

pone-0014357-g007: Phylogenetic comparison of IAPV sequences detected in honeybees and non-Apis hymenopteran pollinators collected near IAPV(+) apiaries.An unrooted maximum likelihood phylogenetic tree of IAPV (based on 771-nt for capsid region) was generated using a region of the structural proteins of the virus. The support for the indicated branching topology was evaluated by using bootstrap re-sampling of the sequences 1,000 times. Nodes supported by bootstrap values over 70 are given. Strains were annotated by genus, species, identification label, country of isolation and year of isolation. Green =  virus from non-Apis hymenopteran pollinators; Black  =  virus sequences from original isolation and honey bees from CCD-affected operations [16]. Non-Apis hymenopteran pollinators collected from same local are indicated by common symbol following sample label.
Mentions: Similarly, for IAPV, an ML phylogenetic tree was inferred using a part of the structural polyprotein for the virus from the Apis mellifera specimens taken from apiaries diagnosed with CCD [16], [17] and the non-Apis pollinators collected from near two of the IAPV-infected apiaries (associated with Operation 3 [16]). The IAPV detected in the non-Apis pollinators did not cluster separately from the IAPV in the honey bees in nearby apiaries (Figure 7) (AI, P = 0.083; PS, P = 1). Interestingly, the viral sequences from non-Apis hymenopteran pollinators collected from the two apiaries segregated by sampling location, with the non-Apis hymenopteran pollinators from each site possessing a clearly phylogenetically distinct lineage of IAPV.

Bottom Line: Although overall pollinator populations have declined over the last couple of decades, the honey bee (Apis mellifera) malady, colony collapse disorder (CCD), has caused major concern in the agricultural community.This finding further expands the viral host range and implies a possible deeper impact on the health of our ecosystem.Notably, in cases where honey bee apiaries affected by CCD harbored honey bees with Israeli Acute Paralysis virus (IAPV), nearby non-Apis hymenopteran pollinators also had IAPV, while those near apiaries without IAPV did not.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, The Pennsylvania State University, Pennsylvania, United States of America.

ABSTRACT
Although overall pollinator populations have declined over the last couple of decades, the honey bee (Apis mellifera) malady, colony collapse disorder (CCD), has caused major concern in the agricultural community. Among honey bee pathogens, RNA viruses are emerging as a serious threat and are suspected as major contributors to CCD. Recent detection of these viral species in bumble bees suggests a possible wider environmental spread of these viruses with potential broader impact. It is therefore vital to study the ecology and epidemiology of these viruses in the hymenopteran pollinator community as a whole. We studied the viral distribution in honey bees, in their pollen loads, and in other non-Apis hymenopteran pollinators collected from flowering plants in Pennsylvania, New York, and Illinois in the United States. Viruses in the samples were detected using reverse transcriptase-PCR and confirmed by sequencing. For the first time, we report the molecular detection of picorna-like RNA viruses (deformed wing virus, sacbrood virus and black queen cell virus) in pollen pellets collected directly from forager bees. Pollen pellets from several uninfected forager bees were detected with virus, indicating that pollen itself may harbor viruses. The viruses in the pollen and honey stored in the hive were demonstrated to be infective, with the queen becoming infected and laying infected eggs after these virus-contaminated foods were given to virus-free colonies. These viruses were detected in eleven other non-Apis hymenopteran species, ranging from many solitary bees to bumble bees and wasps. This finding further expands the viral host range and implies a possible deeper impact on the health of our ecosystem. Phylogenetic analyses support that these viruses are disseminating freely among the pollinators via the flower pollen itself. Notably, in cases where honey bee apiaries affected by CCD harbored honey bees with Israeli Acute Paralysis virus (IAPV), nearby non-Apis hymenopteran pollinators also had IAPV, while those near apiaries without IAPV did not. In containment greenhouse experiments, IAPV moved from infected honey bees to bumble bees and from infected bumble bees to honey bees within a week, demonstrating that the viruses could be transmitted from one species to another. This study adds to our present understanding of virus epidemiology and may help explain bee disease patterns and pollinator population decline in general.

Show MeSH
Related in: MedlinePlus