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Characterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditions.

Garbe J, Wesche A, Bunk B, Kazmierczak M, Selezska K, Rohde C, Sikorski J, Rohde M, Jahn D, Schobert M - BMC Microbiol. (2010)

Bottom Line: The receptor of phage JG024 was determined as lipopolysaccharide.Alginate production was identified as a factor reducing phage infectivity.Phage JG024 is a suitable broad host range phage which could be used in phage therapy.

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Affiliation: Institute of Microbiology, Technische Universit├Ąt Braunschweig, Spielmannstr, 7, 38106 Braunschweig, Germany.

ABSTRACT

Background: Pseudomonas aeruginosa causes lung infections in patients suffering from the genetic disorder Cystic Fibrosis (CF). Once a chronic lung infection is established, P. aeruginosa cannot be eradicated by antibiotic treatment. Phage therapy is an alternative to treat these chronic P. aeruginosa infections. However, little is known about the factors which influence phage infection of P. aeruginosa under infection conditions and suitable broad host range phages.

Results: We isolated and characterized a phage, named JG024, which infects a broad range of clinical and environmental P. aeruginosa strains. Sequencing of the phage genome revealed that the phage JG024 is highly related to the ubiquitous and conserved PB1-like phages. The receptor of phage JG024 was determined as lipopolysaccharide. We used an artificial sputum medium to study phage infection under conditions similar to a chronic lung infection. Alginate production was identified as a factor reducing phage infectivity.

Conclusions: Phage JG024 is a suitable broad host range phage which could be used in phage therapy. Phage infection experiments under simulated chronic lung infection conditions showed that alginate production reduces phage infection efficiency.

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Growth characteristics of JG024. One step growth curve of phage JG024. A representative growth experiment of three independent experiments is shown. The latent phase of JG024 takes approximately 50 min and the phage is able to produce about 180 phage progeny per infected cell.
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Figure 2: Growth characteristics of JG024. One step growth curve of phage JG024. A representative growth experiment of three independent experiments is shown. The latent phase of JG024 takes approximately 50 min and the phage is able to produce about 180 phage progeny per infected cell.

Mentions: To investigate growth parameters like the latent phase and the burst size of the phage JG024, we performed single step growth curves as described in Methods, Figure 2. Phage JG024 has an estimated latent phase of 50 min. The burst size, which describes the mean number of phages liberated per bacterial cell was determined as 180 phages per infected cell.


Characterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditions.

Garbe J, Wesche A, Bunk B, Kazmierczak M, Selezska K, Rohde C, Sikorski J, Rohde M, Jahn D, Schobert M - BMC Microbiol. (2010)

Growth characteristics of JG024. One step growth curve of phage JG024. A representative growth experiment of three independent experiments is shown. The latent phase of JG024 takes approximately 50 min and the phage is able to produce about 180 phage progeny per infected cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3008698&req=5

Figure 2: Growth characteristics of JG024. One step growth curve of phage JG024. A representative growth experiment of three independent experiments is shown. The latent phase of JG024 takes approximately 50 min and the phage is able to produce about 180 phage progeny per infected cell.
Mentions: To investigate growth parameters like the latent phase and the burst size of the phage JG024, we performed single step growth curves as described in Methods, Figure 2. Phage JG024 has an estimated latent phase of 50 min. The burst size, which describes the mean number of phages liberated per bacterial cell was determined as 180 phages per infected cell.

Bottom Line: The receptor of phage JG024 was determined as lipopolysaccharide.Alginate production was identified as a factor reducing phage infectivity.Phage JG024 is a suitable broad host range phage which could be used in phage therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Microbiology, Technische Universit├Ąt Braunschweig, Spielmannstr, 7, 38106 Braunschweig, Germany.

ABSTRACT

Background: Pseudomonas aeruginosa causes lung infections in patients suffering from the genetic disorder Cystic Fibrosis (CF). Once a chronic lung infection is established, P. aeruginosa cannot be eradicated by antibiotic treatment. Phage therapy is an alternative to treat these chronic P. aeruginosa infections. However, little is known about the factors which influence phage infection of P. aeruginosa under infection conditions and suitable broad host range phages.

Results: We isolated and characterized a phage, named JG024, which infects a broad range of clinical and environmental P. aeruginosa strains. Sequencing of the phage genome revealed that the phage JG024 is highly related to the ubiquitous and conserved PB1-like phages. The receptor of phage JG024 was determined as lipopolysaccharide. We used an artificial sputum medium to study phage infection under conditions similar to a chronic lung infection. Alginate production was identified as a factor reducing phage infectivity.

Conclusions: Phage JG024 is a suitable broad host range phage which could be used in phage therapy. Phage infection experiments under simulated chronic lung infection conditions showed that alginate production reduces phage infection efficiency.

Show MeSH
Related in: MedlinePlus