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Malondialdehyde suppresses cerebral function by breaking homeostasis between excitation and inhibition in turtle Trachemys scripta.

Li F, Yang Z, Lu Y, Wei Y, Wang J, Yin D, He R - PLoS ONE (2010)

Bottom Line: These changes were found associated with the deterioration of encoding action potentials in cortical neurons.In addition, MDA increased the ratio of γ-aminobutyric acid to glutamate in turtle's brain, as well as the sensitivity of GABAergic neurons to inputs compared to excitatory neurons.Therefore, MDA, as a metabolic product in the brain, may weaken cerebral function during carbonyl stress through breaking the homeostasis between excitatory and inhibitory neurons.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

ABSTRACT
The levels of malondialdehyde (MDA) are high in the brain during carbonyl stress, such as following daily activities and sleep deprivation. To examine our hypothesis that MDA is one of the major substances in the brain leading to fatigue, the influences of MDA on brain functions and neuronal encodings in red-eared turtle (Trachemys scripta) were studied. The intrathecal injections of MDA brought about sleep-like EEG and fatigue-like behaviors in a dose-dependent manner. These changes were found associated with the deterioration of encoding action potentials in cortical neurons. In addition, MDA increased the ratio of γ-aminobutyric acid to glutamate in turtle's brain, as well as the sensitivity of GABAergic neurons to inputs compared to excitatory neurons. Therefore, MDA, as a metabolic product in the brain, may weaken cerebral function during carbonyl stress through breaking the homeostasis between excitatory and inhibitory neurons.

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Related in: MedlinePlus

HPLC analysis of Glu and GABA content in turtles' brain under MDA treatment.Chromatograms were recorded in 5 minutes (panel a) and 35 minutes (panel b), under 20 µM MDA treatment. Then, chromatograms were recorded in 35 minutes under the treatment of 2 µM MDA (panel c); 0.2 µM MDA (panel d); and the phosphate buffer as control (panel e). The HPLC peak area was estimated to calculate the ratio of [GABA]/[Glu] from the brain extract of turtles at different time intervals under the MDA and control treatments (panel f).
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pone-0015325-g005: HPLC analysis of Glu and GABA content in turtles' brain under MDA treatment.Chromatograms were recorded in 5 minutes (panel a) and 35 minutes (panel b), under 20 µM MDA treatment. Then, chromatograms were recorded in 35 minutes under the treatment of 2 µM MDA (panel c); 0.2 µM MDA (panel d); and the phosphate buffer as control (panel e). The HPLC peak area was estimated to calculate the ratio of [GABA]/[Glu] from the brain extract of turtles at different time intervals under the MDA and control treatments (panel f).

Mentions: Glu and GABA in a standard mixture of amino acids were clearly separated within 24 minutes. The retention time for Glu and GABA was about 15.8 minutes and 21.3 minutes, respectively (Fig. 5). The HPLC peak areas of GABA and Glu in the extract of turtle brain did not show significant changes in 5 minutes under 20 µM MDA treatment (Fig. 5a) compared to control group (Fig. 5e). In 35 minutes under the MDA treatment, corresponding to the strongest moment of slow wave EEG, the peak areas of both GABA and Glu showed statistical increment (p<0.01) (Fig. 5b, Tab. 3). The peak area of GABA increased much faster than that of Glu, thus the ratio between GABA and Glu increased significantly (p<0.01) (Fig. 5f). The turtles treated with 2 µM MDA showed a similar change but weaker than the 20 µM MDA treatment (Fig. 5c). However, the group treated with 0.2 µM MDA showed no statistical changes (Fig. 5d) compared to the control group (Tab. 3).


Malondialdehyde suppresses cerebral function by breaking homeostasis between excitation and inhibition in turtle Trachemys scripta.

Li F, Yang Z, Lu Y, Wei Y, Wang J, Yin D, He R - PLoS ONE (2010)

HPLC analysis of Glu and GABA content in turtles' brain under MDA treatment.Chromatograms were recorded in 5 minutes (panel a) and 35 minutes (panel b), under 20 µM MDA treatment. Then, chromatograms were recorded in 35 minutes under the treatment of 2 µM MDA (panel c); 0.2 µM MDA (panel d); and the phosphate buffer as control (panel e). The HPLC peak area was estimated to calculate the ratio of [GABA]/[Glu] from the brain extract of turtles at different time intervals under the MDA and control treatments (panel f).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3008675&req=5

pone-0015325-g005: HPLC analysis of Glu and GABA content in turtles' brain under MDA treatment.Chromatograms were recorded in 5 minutes (panel a) and 35 minutes (panel b), under 20 µM MDA treatment. Then, chromatograms were recorded in 35 minutes under the treatment of 2 µM MDA (panel c); 0.2 µM MDA (panel d); and the phosphate buffer as control (panel e). The HPLC peak area was estimated to calculate the ratio of [GABA]/[Glu] from the brain extract of turtles at different time intervals under the MDA and control treatments (panel f).
Mentions: Glu and GABA in a standard mixture of amino acids were clearly separated within 24 minutes. The retention time for Glu and GABA was about 15.8 minutes and 21.3 minutes, respectively (Fig. 5). The HPLC peak areas of GABA and Glu in the extract of turtle brain did not show significant changes in 5 minutes under 20 µM MDA treatment (Fig. 5a) compared to control group (Fig. 5e). In 35 minutes under the MDA treatment, corresponding to the strongest moment of slow wave EEG, the peak areas of both GABA and Glu showed statistical increment (p<0.01) (Fig. 5b, Tab. 3). The peak area of GABA increased much faster than that of Glu, thus the ratio between GABA and Glu increased significantly (p<0.01) (Fig. 5f). The turtles treated with 2 µM MDA showed a similar change but weaker than the 20 µM MDA treatment (Fig. 5c). However, the group treated with 0.2 µM MDA showed no statistical changes (Fig. 5d) compared to the control group (Tab. 3).

Bottom Line: These changes were found associated with the deterioration of encoding action potentials in cortical neurons.In addition, MDA increased the ratio of γ-aminobutyric acid to glutamate in turtle's brain, as well as the sensitivity of GABAergic neurons to inputs compared to excitatory neurons.Therefore, MDA, as a metabolic product in the brain, may weaken cerebral function during carbonyl stress through breaking the homeostasis between excitatory and inhibitory neurons.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

ABSTRACT
The levels of malondialdehyde (MDA) are high in the brain during carbonyl stress, such as following daily activities and sleep deprivation. To examine our hypothesis that MDA is one of the major substances in the brain leading to fatigue, the influences of MDA on brain functions and neuronal encodings in red-eared turtle (Trachemys scripta) were studied. The intrathecal injections of MDA brought about sleep-like EEG and fatigue-like behaviors in a dose-dependent manner. These changes were found associated with the deterioration of encoding action potentials in cortical neurons. In addition, MDA increased the ratio of γ-aminobutyric acid to glutamate in turtle's brain, as well as the sensitivity of GABAergic neurons to inputs compared to excitatory neurons. Therefore, MDA, as a metabolic product in the brain, may weaken cerebral function during carbonyl stress through breaking the homeostasis between excitatory and inhibitory neurons.

Show MeSH
Related in: MedlinePlus