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Quantitative promoter methylation differentiates carcinoma ex pleomorphic adenoma from pleomorphic salivary adenoma.

Schache AG, Hall G, Woolgar JA, Nikolaidis G, Triantafyllou A, Lowe D, Risk JM, Shaw RJ, Liloglou T - Br. J. Cancer (2010)

Bottom Line: Previous analysis has been limited by a non-quantitative approach.We sought to demonstrate quantitative promoter methylation across a panel of tumour suppressor genes (TSGs) in both Ca ex PSA and PSA. quantitative methylation-specific real-time polymerase chain reaction (qMSP) analysis of p16(INK4A), CYGB, RASSF1, RARβ, human telomerase reverse transcriptase (hTERT), Wilms' tumour 1 (WT1) and TMEFF2 gene promoters was undertaken on bisulphite-converted DNA, previously extracted from archival fixed tissue specimens of 31 Ca ex PSA and an unrelated cohort of 28 PSA.RASSF1 was the single gene promoter for which methylation is shown to be a statistically significant predictor of malignant disease (P<0.001) with a sensitivity of 51.6% and a specificity of 92.9%.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular & Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Daulby Street, Liverpool L69 3GN, UK. schache@liverpool.ac.uk

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qMSP results. Representation of qMSP data for Ca ex PSA (left) and PSA (right) samples. ▪, Promoter methylation positive;  Promoter methylation negative (at 5% methylation threshold). Data from hTERT, WT1, RASSF1 and p16 data is from duplicate experiments.
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fig1: qMSP results. Representation of qMSP data for Ca ex PSA (left) and PSA (right) samples. ▪, Promoter methylation positive; Promoter methylation negative (at 5% methylation threshold). Data from hTERT, WT1, RASSF1 and p16 data is from duplicate experiments.

Mentions: Promoter methylation was observed in four of the seven gene promoters included on the methylation panel (Figure 1). Methylation was demonstrated in a total of 20 out of 31 Ca ex PSA samples (64.5%) and 2 out of 28 (7.1%) PSA samples (P<0.001). The hTERT, WT1 and p16 INK4A methylation showed 12.9%, 9.7% and 12.9% sensitivity, respectively, and 100% specificity (Table 4). RARβ, CYGB and TMEFF2 methylation was not apparent in any sample (Figure 1). RASSF1 was the single gene promoter for which methylation is shown to be a statistically significant predictor of malignant disease (P<0.001) with a sensitivity of 51.6% and a specificity of 92.9% (Table 4).


Quantitative promoter methylation differentiates carcinoma ex pleomorphic adenoma from pleomorphic salivary adenoma.

Schache AG, Hall G, Woolgar JA, Nikolaidis G, Triantafyllou A, Lowe D, Risk JM, Shaw RJ, Liloglou T - Br. J. Cancer (2010)

qMSP results. Representation of qMSP data for Ca ex PSA (left) and PSA (right) samples. ▪, Promoter methylation positive;  Promoter methylation negative (at 5% methylation threshold). Data from hTERT, WT1, RASSF1 and p16 data is from duplicate experiments.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3008600&req=5

fig1: qMSP results. Representation of qMSP data for Ca ex PSA (left) and PSA (right) samples. ▪, Promoter methylation positive; Promoter methylation negative (at 5% methylation threshold). Data from hTERT, WT1, RASSF1 and p16 data is from duplicate experiments.
Mentions: Promoter methylation was observed in four of the seven gene promoters included on the methylation panel (Figure 1). Methylation was demonstrated in a total of 20 out of 31 Ca ex PSA samples (64.5%) and 2 out of 28 (7.1%) PSA samples (P<0.001). The hTERT, WT1 and p16 INK4A methylation showed 12.9%, 9.7% and 12.9% sensitivity, respectively, and 100% specificity (Table 4). RARβ, CYGB and TMEFF2 methylation was not apparent in any sample (Figure 1). RASSF1 was the single gene promoter for which methylation is shown to be a statistically significant predictor of malignant disease (P<0.001) with a sensitivity of 51.6% and a specificity of 92.9% (Table 4).

Bottom Line: Previous analysis has been limited by a non-quantitative approach.We sought to demonstrate quantitative promoter methylation across a panel of tumour suppressor genes (TSGs) in both Ca ex PSA and PSA. quantitative methylation-specific real-time polymerase chain reaction (qMSP) analysis of p16(INK4A), CYGB, RASSF1, RARβ, human telomerase reverse transcriptase (hTERT), Wilms' tumour 1 (WT1) and TMEFF2 gene promoters was undertaken on bisulphite-converted DNA, previously extracted from archival fixed tissue specimens of 31 Ca ex PSA and an unrelated cohort of 28 PSA.RASSF1 was the single gene promoter for which methylation is shown to be a statistically significant predictor of malignant disease (P<0.001) with a sensitivity of 51.6% and a specificity of 92.9%.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular & Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Daulby Street, Liverpool L69 3GN, UK. schache@liverpool.ac.uk

Show MeSH
Related in: MedlinePlus