Limits...
Effect of marine polyunsaturated fatty acids on biofilm formation of Candida albicans and Candida dubliniensis.

Thibane VS, Kock JL, Ells R, van Wyk PW, Pohl CH - Mar Drugs (2010)

Bottom Line: The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated.It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans.These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, 9301, South Africa. ThibaneV@ufs.ac.za

ABSTRACT
The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated. It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans. Ultrastructural differences, which may be due to increased oxidative stress, were observed between treated and untreated cells of C. albicans and C. dubliniensis with formation of rough cell walls by both species and fibrillar structures in C. dubliniensis. These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

Show MeSH
SEM micrographs showing cells of C. dubliniensis control biofilms (a) and biofilms treated with 1mM 18:4 n-3 (b), 20:5 n-3 (c) and 22:5 n-3 (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC2992994&req=5

f4-marinedrugs-08-02597: SEM micrographs showing cells of C. dubliniensis control biofilms (a) and biofilms treated with 1mM 18:4 n-3 (b), 20:5 n-3 (c) and 22:5 n-3 (d).

Mentions: Figure 3 depicts biofilms of C. albicans grown in the presence and absence of 1 mM 18:4 n-3, 20:5 n-3 and 22:5 n-3. In the absence of the PUFAs, the cell surface appeared smooth (Figure 3a) and when grown in the presence of PUFAs, cells had a rough appearance with protuberances (Figure 3b, c, d). Similar results were also observed for biofilms of C. dubliniensis when grown in the absence (Figure 4a), and the presence (Figure 4b, c, d), of the PUFAs with protuberances and fibrillar structures visible on the cell surface. Similar rough cell surfaces were observed when C. albicans was exposed to miconazole [20], which is known to cause an increase in reactive oxygen species in Candida biofilm cells [21]. Lemar et al. [22] also found that C. albicans cells were not smooth in the presence of alyl alcohol, which increases oxidative stress. Furthermore, in a study by Leeuw et al. [23] on the yeast Cryptococcus curvatus grown on oxidised lipids, protuberances were observed on the cell surface. We therefore speculate that the changes in cell surface in the presence of PUFAs might be due to increased lipid peroxidation and resultant oxidative stress. This will be studied in future.


Effect of marine polyunsaturated fatty acids on biofilm formation of Candida albicans and Candida dubliniensis.

Thibane VS, Kock JL, Ells R, van Wyk PW, Pohl CH - Mar Drugs (2010)

SEM micrographs showing cells of C. dubliniensis control biofilms (a) and biofilms treated with 1mM 18:4 n-3 (b), 20:5 n-3 (c) and 22:5 n-3 (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2992994&req=5

f4-marinedrugs-08-02597: SEM micrographs showing cells of C. dubliniensis control biofilms (a) and biofilms treated with 1mM 18:4 n-3 (b), 20:5 n-3 (c) and 22:5 n-3 (d).
Mentions: Figure 3 depicts biofilms of C. albicans grown in the presence and absence of 1 mM 18:4 n-3, 20:5 n-3 and 22:5 n-3. In the absence of the PUFAs, the cell surface appeared smooth (Figure 3a) and when grown in the presence of PUFAs, cells had a rough appearance with protuberances (Figure 3b, c, d). Similar results were also observed for biofilms of C. dubliniensis when grown in the absence (Figure 4a), and the presence (Figure 4b, c, d), of the PUFAs with protuberances and fibrillar structures visible on the cell surface. Similar rough cell surfaces were observed when C. albicans was exposed to miconazole [20], which is known to cause an increase in reactive oxygen species in Candida biofilm cells [21]. Lemar et al. [22] also found that C. albicans cells were not smooth in the presence of alyl alcohol, which increases oxidative stress. Furthermore, in a study by Leeuw et al. [23] on the yeast Cryptococcus curvatus grown on oxidised lipids, protuberances were observed on the cell surface. We therefore speculate that the changes in cell surface in the presence of PUFAs might be due to increased lipid peroxidation and resultant oxidative stress. This will be studied in future.

Bottom Line: The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated.It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans.These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, 9301, South Africa. ThibaneV@ufs.ac.za

ABSTRACT
The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated. It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans. Ultrastructural differences, which may be due to increased oxidative stress, were observed between treated and untreated cells of C. albicans and C. dubliniensis with formation of rough cell walls by both species and fibrillar structures in C. dubliniensis. These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

Show MeSH