Limits...
Effect of marine polyunsaturated fatty acids on biofilm formation of Candida albicans and Candida dubliniensis.

Thibane VS, Kock JL, Ells R, van Wyk PW, Pohl CH - Mar Drugs (2010)

Bottom Line: The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated.It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans.These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, 9301, South Africa. ThibaneV@ufs.ac.za

ABSTRACT
The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated. It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans. Ultrastructural differences, which may be due to increased oxidative stress, were observed between treated and untreated cells of C. albicans and C. dubliniensis with formation of rough cell walls by both species and fibrillar structures in C. dubliniensis. These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

Show MeSH
Effect of marine fatty acids (18:4 n-3, 20:4 n-3, 20:5 n-3, 22:5 n-3, 22:6 n-3) on mitochondrial metabolism of C. albicans and C. dubliniensis biofilms. Biofilms were grown in the presence of 1 mM of the fatty acids and mitochondrial activity was monitored using the XTT assay. The percentage inhibition values were determined compared to untreated controls. n = 8; * significantly different from control (P ≤ 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC2992994&req=5

f1-marinedrugs-08-02597: Effect of marine fatty acids (18:4 n-3, 20:4 n-3, 20:5 n-3, 22:5 n-3, 22:6 n-3) on mitochondrial metabolism of C. albicans and C. dubliniensis biofilms. Biofilms were grown in the presence of 1 mM of the fatty acids and mitochondrial activity was monitored using the XTT assay. The percentage inhibition values were determined compared to untreated controls. n = 8; * significantly different from control (P ≤ 0.01).

Mentions: As indicated in Figure 1 the mitochondrial metabolism of biofilms of C. albicans and C. dubliniensis, assessed by XTT assay, was significantly inhibited by 1 mM 18:4 n-3, 20:5 n-3 and 22:5 n-3. In addition, biofilms of C. albicans were also sensitive to 20:4 n-6, while biofilms of C. dubliniensis were not significantly inhibited. Interestingly, 22:6 n-3 did not significantly inhibit mitochondrial metabolism of C. albicans or C. dubliniensis biofilms. As a result 20:4 n-6 and 22:6 n-3 were not used in further experiments.


Effect of marine polyunsaturated fatty acids on biofilm formation of Candida albicans and Candida dubliniensis.

Thibane VS, Kock JL, Ells R, van Wyk PW, Pohl CH - Mar Drugs (2010)

Effect of marine fatty acids (18:4 n-3, 20:4 n-3, 20:5 n-3, 22:5 n-3, 22:6 n-3) on mitochondrial metabolism of C. albicans and C. dubliniensis biofilms. Biofilms were grown in the presence of 1 mM of the fatty acids and mitochondrial activity was monitored using the XTT assay. The percentage inhibition values were determined compared to untreated controls. n = 8; * significantly different from control (P ≤ 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2992994&req=5

f1-marinedrugs-08-02597: Effect of marine fatty acids (18:4 n-3, 20:4 n-3, 20:5 n-3, 22:5 n-3, 22:6 n-3) on mitochondrial metabolism of C. albicans and C. dubliniensis biofilms. Biofilms were grown in the presence of 1 mM of the fatty acids and mitochondrial activity was monitored using the XTT assay. The percentage inhibition values were determined compared to untreated controls. n = 8; * significantly different from control (P ≤ 0.01).
Mentions: As indicated in Figure 1 the mitochondrial metabolism of biofilms of C. albicans and C. dubliniensis, assessed by XTT assay, was significantly inhibited by 1 mM 18:4 n-3, 20:5 n-3 and 22:5 n-3. In addition, biofilms of C. albicans were also sensitive to 20:4 n-6, while biofilms of C. dubliniensis were not significantly inhibited. Interestingly, 22:6 n-3 did not significantly inhibit mitochondrial metabolism of C. albicans or C. dubliniensis biofilms. As a result 20:4 n-6 and 22:6 n-3 were not used in further experiments.

Bottom Line: The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated.It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans.These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, 9301, South Africa. ThibaneV@ufs.ac.za

ABSTRACT
The effect of marine polyunsaturated fatty acids on biofilm formation by the human pathogens Candida albicans and Candida dubliniensis was investigated. It was found that stearidonic acid (18:4 n-3), eicosapentaenoic acid (20:5 n-3), docosapentaenoic acid (22:5 n-3) and docosahexaenoic acid (22:6 n-3) have an inhibitory effect on mitochondrial metabolism of both C. albicans and C. dubliniensis and that the production of biofilm biomass by C. dubliniensis was more susceptible to these fatty acids than C. albicans. Ultrastructural differences, which may be due to increased oxidative stress, were observed between treated and untreated cells of C. albicans and C. dubliniensis with formation of rough cell walls by both species and fibrillar structures in C. dubliniensis. These results indicate that marine polyunsaturated fatty acids may be useful in the treatment and/or prevention of biofilms formed by these pathogenic yeasts.

Show MeSH