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Increased levels of vascular endothelial growth factor in the aqueous humor of patients with diabetic retinopathy.

Selim KM, Sahan D, Muhittin T, Osman C, Mustafa O - Indian J Ophthalmol (2010 Sep-Oct)

Bottom Line: A strong statistical correlation was found to exist between the level of aqueous VEGF and the severity of DR ( P < 0.001), whereas, the VEGF levels in a control group and a diabetic group without DR were not significantly different ( P = 0.985).On the other hand, a statistically insignificant ( P = 0.065) correlation was found between elevated aqueous VEGF and the presence of macular edema in the NPDR group.VEGF was elevated in the aqueous humor of patients with DR compared to that in normal eyes.

View Article: PubMed Central - PubMed

Affiliation: Ophthalmology Department, Vakif Gureba Education and Research Hospital, Istanbul, Turkey.

ABSTRACT

Purpose: This study aims to investigate the levels of aqueous vascular endothelial growth factor (VEGF) in diabetic patient groups in comparison to normal subjects, and to correlate elevated VEGF with the severity of diabetic retinopathy (DR).

Materials and methods: Aqueous samples were obtained from 78 eyes of 74 patients undergoing intraocular surgery and they were examined by the enzyme-linked immunosorbent assay. Color photographs, optical coherence tomography scans, and fluorescein angiography were used to evaluate patients preoperatively.

Results: A strong statistical correlation was found to exist between the level of aqueous VEGF and the severity of DR ( P < 0.001), whereas, the VEGF levels in a control group and a diabetic group without DR were not significantly different ( P = 0.985). Aqueous VEGF levels were significantly elevated in patients with proliferative DR (PDR) as compared to the control group ( P < 0.001), to diabetic patients without retinopathy (NDR) ( P < 0.001), and to diabetic patients with nonproliferative DR (NPDR) ( P < 0.001). The aqueous VEGF levels were significantly higher in patients with active PDR than in those with quiescent PDR ( P = 0.001). On the other hand, a statistically insignificant ( P = 0.065) correlation was found between elevated aqueous VEGF and the presence of macular edema in the NPDR group.

Conclusions: VEGF was elevated in the aqueous humor of patients with DR compared to that in normal eyes. The aqueous VEGF level had a strong correlation with the severity of retinopathy along with a statistically insignificant difference in macular edema.

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Related in: MedlinePlus

Diagram graphically displaying the differences between the aqueous VEGF levels of subgroups with diabetic retinopathy on boxplots C1= NPDR Subgroup without CSDME, C2= NPDR Subgroup with CSDME, D1= Active PDR Subgroup, D2= Quiescent PDR Subgroup
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Figure 0002: Diagram graphically displaying the differences between the aqueous VEGF levels of subgroups with diabetic retinopathy on boxplots C1= NPDR Subgroup without CSDME, C2= NPDR Subgroup with CSDME, D1= Active PDR Subgroup, D2= Quiescent PDR Subgroup

Mentions: Statistical analysis of the aqueous VEGF levels of the NPDR subgroups showed a difference that was not significant statistically (Mann-Whitney U test; two-tailed P = 0,065), with comparable values in subgroup C1 (without CS-DME, 113.7 ± 49.63 pg/ml) and subgroup C2 (with CS-DME, 154.4 ± 61.32 pg/ml). On the other hand, a statistically significant difference (Mann-Whitney U test; two-tailed P < 0.001) was found between the aqueous VEGF levels of subgroups D1 (Active PDR, 466.18 ± 242.84 pg/ml) and D2 (Quiescent PDR, 143.12 ± 66.86 pg/ml) [Table 3 and Fig. 2].


Increased levels of vascular endothelial growth factor in the aqueous humor of patients with diabetic retinopathy.

Selim KM, Sahan D, Muhittin T, Osman C, Mustafa O - Indian J Ophthalmol (2010 Sep-Oct)

Diagram graphically displaying the differences between the aqueous VEGF levels of subgroups with diabetic retinopathy on boxplots C1= NPDR Subgroup without CSDME, C2= NPDR Subgroup with CSDME, D1= Active PDR Subgroup, D2= Quiescent PDR Subgroup
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2992910&req=5

Figure 0002: Diagram graphically displaying the differences between the aqueous VEGF levels of subgroups with diabetic retinopathy on boxplots C1= NPDR Subgroup without CSDME, C2= NPDR Subgroup with CSDME, D1= Active PDR Subgroup, D2= Quiescent PDR Subgroup
Mentions: Statistical analysis of the aqueous VEGF levels of the NPDR subgroups showed a difference that was not significant statistically (Mann-Whitney U test; two-tailed P = 0,065), with comparable values in subgroup C1 (without CS-DME, 113.7 ± 49.63 pg/ml) and subgroup C2 (with CS-DME, 154.4 ± 61.32 pg/ml). On the other hand, a statistically significant difference (Mann-Whitney U test; two-tailed P < 0.001) was found between the aqueous VEGF levels of subgroups D1 (Active PDR, 466.18 ± 242.84 pg/ml) and D2 (Quiescent PDR, 143.12 ± 66.86 pg/ml) [Table 3 and Fig. 2].

Bottom Line: A strong statistical correlation was found to exist between the level of aqueous VEGF and the severity of DR ( P < 0.001), whereas, the VEGF levels in a control group and a diabetic group without DR were not significantly different ( P = 0.985).On the other hand, a statistically insignificant ( P = 0.065) correlation was found between elevated aqueous VEGF and the presence of macular edema in the NPDR group.VEGF was elevated in the aqueous humor of patients with DR compared to that in normal eyes.

View Article: PubMed Central - PubMed

Affiliation: Ophthalmology Department, Vakif Gureba Education and Research Hospital, Istanbul, Turkey.

ABSTRACT

Purpose: This study aims to investigate the levels of aqueous vascular endothelial growth factor (VEGF) in diabetic patient groups in comparison to normal subjects, and to correlate elevated VEGF with the severity of diabetic retinopathy (DR).

Materials and methods: Aqueous samples were obtained from 78 eyes of 74 patients undergoing intraocular surgery and they were examined by the enzyme-linked immunosorbent assay. Color photographs, optical coherence tomography scans, and fluorescein angiography were used to evaluate patients preoperatively.

Results: A strong statistical correlation was found to exist between the level of aqueous VEGF and the severity of DR ( P < 0.001), whereas, the VEGF levels in a control group and a diabetic group without DR were not significantly different ( P = 0.985). Aqueous VEGF levels were significantly elevated in patients with proliferative DR (PDR) as compared to the control group ( P < 0.001), to diabetic patients without retinopathy (NDR) ( P < 0.001), and to diabetic patients with nonproliferative DR (NPDR) ( P < 0.001). The aqueous VEGF levels were significantly higher in patients with active PDR than in those with quiescent PDR ( P = 0.001). On the other hand, a statistically insignificant ( P = 0.065) correlation was found between elevated aqueous VEGF and the presence of macular edema in the NPDR group.

Conclusions: VEGF was elevated in the aqueous humor of patients with DR compared to that in normal eyes. The aqueous VEGF level had a strong correlation with the severity of retinopathy along with a statistically insignificant difference in macular edema.

Show MeSH
Related in: MedlinePlus