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Isolation of BAC clones containing conserved genes from libraries of three distantly related moths: a useful resource for comparative genomics of Lepidoptera.

Yasukochi Y, Tanaka-Okuyama M, Kamimura M, Nakano R, Naito Y, Ishikawa Y, Sahara K - J. Biomed. Biotechnol. (2010)

Bottom Line: A total of 108-184 clones representing 101-182 conserved genes were isolated for each species.For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species.The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.

View Article: PubMed Central - PubMed

Affiliation: Insect Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. yaskoch@affrc.go.jp

ABSTRACT
Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, n = 31, which are not closely related with each other or with the silkworm, Bombyx mori, (n = 28), the sequenced model lepidopteran. A total of 108-184 clones representing 101-182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.

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Schematic representation of phylogeny of Lepidoptera and haploid chromosome numbers of species referred to in the text. The phylogeny is based on the studies of Regier et al. [18]. Neither Bombycoidea nor Noctuidae are monophyletic, but form a clade clearly distinguishable from Pyraloidea and butterflies including Papilionoidea [18].
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fig1: Schematic representation of phylogeny of Lepidoptera and haploid chromosome numbers of species referred to in the text. The phylogeny is based on the studies of Regier et al. [18]. Neither Bombycoidea nor Noctuidae are monophyletic, but form a clade clearly distinguishable from Pyraloidea and butterflies including Papilionoidea [18].

Mentions: Noctuoidea is the largest family of Lepidoptera and includes many serious and globally distributed agricultural pests. Therefore, we selected the tobacco budworm, Heliothis virescens, as a target for our studies since a BAC library [7] and more than 60,000 expressed sequenced tags (ESTs) [13–15] were available. The European corn borer, Ostrinia nubilalis, was also appropriate for this analysis due to the availability of a BAC library (http://www.genome.clemson.edu/services) and ESTs [16, 17]. O. nubilalis belongs to the superfamily, Pyraloidea, which forms a different clade from Bombycoidea and Noctuidae but is closer to them than butterflies (Figure 1) [18]. Another species with available BACs was the diamondback moth, Plutella xylostella, which is well known to develop resistance rapidly to a wide variety of insecticides [19–21]. P. xylostella belongs to the superfamily, Yponomeutoidea, which is primitive compared with Macrolepidoptera, which contains the other two species, but all belong to the same major group of advanced Lepidoptera, Ditrysia (Figure 1) [18].


Isolation of BAC clones containing conserved genes from libraries of three distantly related moths: a useful resource for comparative genomics of Lepidoptera.

Yasukochi Y, Tanaka-Okuyama M, Kamimura M, Nakano R, Naito Y, Ishikawa Y, Sahara K - J. Biomed. Biotechnol. (2010)

Schematic representation of phylogeny of Lepidoptera and haploid chromosome numbers of species referred to in the text. The phylogeny is based on the studies of Regier et al. [18]. Neither Bombycoidea nor Noctuidae are monophyletic, but form a clade clearly distinguishable from Pyraloidea and butterflies including Papilionoidea [18].
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2992816&req=5

fig1: Schematic representation of phylogeny of Lepidoptera and haploid chromosome numbers of species referred to in the text. The phylogeny is based on the studies of Regier et al. [18]. Neither Bombycoidea nor Noctuidae are monophyletic, but form a clade clearly distinguishable from Pyraloidea and butterflies including Papilionoidea [18].
Mentions: Noctuoidea is the largest family of Lepidoptera and includes many serious and globally distributed agricultural pests. Therefore, we selected the tobacco budworm, Heliothis virescens, as a target for our studies since a BAC library [7] and more than 60,000 expressed sequenced tags (ESTs) [13–15] were available. The European corn borer, Ostrinia nubilalis, was also appropriate for this analysis due to the availability of a BAC library (http://www.genome.clemson.edu/services) and ESTs [16, 17]. O. nubilalis belongs to the superfamily, Pyraloidea, which forms a different clade from Bombycoidea and Noctuidae but is closer to them than butterflies (Figure 1) [18]. Another species with available BACs was the diamondback moth, Plutella xylostella, which is well known to develop resistance rapidly to a wide variety of insecticides [19–21]. P. xylostella belongs to the superfamily, Yponomeutoidea, which is primitive compared with Macrolepidoptera, which contains the other two species, but all belong to the same major group of advanced Lepidoptera, Ditrysia (Figure 1) [18].

Bottom Line: A total of 108-184 clones representing 101-182 conserved genes were isolated for each species.For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species.The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.

View Article: PubMed Central - PubMed

Affiliation: Insect Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. yaskoch@affrc.go.jp

ABSTRACT
Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, n = 31, which are not closely related with each other or with the silkworm, Bombyx mori, (n = 28), the sequenced model lepidopteran. A total of 108-184 clones representing 101-182 conserved genes were isolated for each species. For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species. The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.

Show MeSH
Related in: MedlinePlus